1Transcription
Process of copying genetic info from DNA to RNA
2Translation
Process by which genetic info encoded in RNA is used to make proteins
3Replication
Process of copying DNA to make an identical copy, which occurs before cell division.
4Central Dogma
DNA is transcribed into RNA which is translated into protein
5Amino acid / number of amino acids
Building blocks of proteins
20 Types of amino acids
6Protein
Molecules made up of amino acids
7Polypeptide
Chain of amino acids not folded into protein
8Peptide Bond
Covalent bond that links amino acids in polypeptide chain
9Codon/number of combinations with and without stop codons
Three-letter sequence of RNA that codes for specific amino acid
64 possible codon combinations, 3 stop codons
10Anticodon
Three nucleotides on tRNA that pair with complementary codon on mRNA
11Ribosome
Alings RNA and links amino acids
12tRNA
Type of RNA that carries amino acids to ribosome
13mRNA
Type of RNA that carries genetic info from DNA to ribosome
14rRNA
Type of RNA that forms structure of ribosome
15RNA (sugar, structure, function, location)
Ribose, Single-stranded, transfers genetic info from DNA to ribosome, nucleus and cytoplasm.
16DNA (sugar, structure, function, location in animal and bacterial cells)
Deoxyribose, double-stranded, contains genetic info, nucleus of animal cells, nucleoid region of bacterial cells.
17Exon
Codes for part of protein and retained in final mRNA after splicing.
18Intron
Does not code for protein and removed from initial RNA transcript during splicing. (JUNK GENES)
19pre-mRNA
Initial RNA produced during transcription and undergoes splicing to remove introns and form mRNA.
20Spliceosome
Carries out the splicing of pre-mRNA
21Nucleolus / Nuclear Pore
Nucleolus is a region within nucleus where ribosomes are assembled
Nuclear pore is complex of proteins that regulates transport of molecules between nucleus and cytoplasm
22Free ribosomes vs Fixed ribosomes
Free ribosomes: Ribosomes located in cytoplasm and produce proteins that function within cell
Fixed ribosomes: Attached to endoplasmic reticulum and produce proteins that are exported
23A. P. E. sites in rRNA
A site is where next tRNA molecule binds to ribosome
P site is where polypeptide chain is located
E site is where spent tRNA molecule is released
24Initiator Codon
Initiator codon (AUG) signals start of protein synthesis.
25Terminator in DNA
Signals end of transcription
26Promotor
Signals start of transcription by binding to RNA polymerase
27RNA Polymerase
Transcribes DNA into RNA
28Poly-A Tail and Cap
mRNA reads 5' to 3'
Poly-A Tail 3' end
Cap 5' end
29RNA subunits for eukaryotic and prokaryotic cells
Large and Small
30What are the 3 steps in translation?
Translation has three steps: initiation, elongation, and termination (IET)
Initiation: Ribosome assembles around mRNA and first tRNA molecule is recruited
Elongation: Ribosome moves along mRNA, adding new amino acids to growing polypeptide chain
Termination: Ribosome reaches stop codon and completed polypeptide chain is released
31Gene
Codes for functional RNA or protein
32Mutation
Change in DNA sequence of gene that alters function of encoded protein or RNA
Arise spontaneously or induced by environmental factors such as radiation or chemicals