Protein Synthesis Test

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1
Transcription
Process of copying genetic info from DNA to RNA
2
Translation
Process by which genetic info encoded in RNA is used to make proteins
3
Replication
Process of copying DNA to make an identical copy, which occurs before cell division.
4
Central Dogma
DNA is transcribed into RNA which is translated into protein
5
Amino acid / number of amino acids
Building blocks of proteins
20 Types of amino acids
6
Protein
Molecules made up of amino acids
7
Polypeptide
Chain of amino acids not folded into protein
8
Peptide Bond
Covalent bond that links amino acids in polypeptide chain
9
Codon/number of combinations with and without stop codons
Three-letter sequence of RNA that codes for specific amino acid
64 possible codon combinations, 3 stop codons
10
Anticodon
Three nucleotides on tRNA that pair with complementary codon on mRNA
11
Ribosome
Alings RNA and links amino acids
12
tRNA
Type of RNA that carries amino acids to ribosome
13
mRNA
Type of RNA that carries genetic info from DNA to ribosome
14
rRNA
Type of RNA that forms structure of ribosome
15
RNA (sugar, structure, function, location)
Ribose, Single-stranded, transfers genetic info from DNA to ribosome, nucleus and cytoplasm.
16
DNA (sugar, structure, function, location in animal and bacterial cells)
Deoxyribose, double-stranded, contains genetic info, nucleus of animal cells, nucleoid region of bacterial cells.
17
Exon
Codes for part of protein and retained in final mRNA after splicing.
18
Intron
Does not code for protein and removed from initial RNA transcript during splicing. (JUNK GENES)
19
pre-mRNA
Initial RNA produced during transcription and undergoes splicing to remove introns and form mRNA.
20
Spliceosome
Carries out the splicing of pre-mRNA
21
Nucleolus / Nuclear Pore
Nucleolus is a region within nucleus where ribosomes are assembled
Nuclear pore is complex of proteins that regulates transport of molecules between nucleus and cytoplasm
22
Free ribosomes vs Fixed ribosomes
Free ribosomes: Ribosomes located in cytoplasm and produce proteins that function within cell
Fixed ribosomes: Attached to endoplasmic reticulum and produce proteins that are exported
23
A. P. E. sites in rRNA
A site is where next tRNA molecule binds to ribosome
P site is where polypeptide chain is located
E site is where spent tRNA molecule is released
24
Initiator Codon
Initiator codon (AUG) signals start of protein synthesis.
25
Terminator in DNA
Signals end of transcription
26
Promotor
Signals start of transcription by binding to RNA polymerase
27
RNA Polymerase
Transcribes DNA into RNA
28
Poly-A Tail and Cap
mRNA reads 5' to 3'
Poly-A Tail 3' end
Cap 5' end
29
RNA subunits for eukaryotic and prokaryotic cells
Large and Small
30
What are the 3 steps in translation?
Translation has three steps: initiation, elongation, and termination (IET)
Initiation: Ribosome assembles around mRNA and first tRNA molecule is recruited
Elongation: Ribosome moves along mRNA, adding new amino acids to growing polypeptide chain
Termination: Ribosome reaches stop codon and completed polypeptide chain is released
31
Gene
Codes for functional RNA or protein
32
Mutation
Change in DNA sequence of gene that alters function of encoded protein or RNA
Arise spontaneously or induced by environmental factors such as radiation or chemicals
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