1Role of RNA poly 1? where is it found?
-transcribes rRNA genes
-located in nucleolus
2Types of RNA poly 1?
5.8 S
18S
28 S
3Role of RNA poly 3?
-transcribes tRNA genes
-rRNA genes
-some snRNA
-genes for other small rna's
4Role of RNA poly 2? where is it found?
-transcribes all protein coding genes
-snoRNA genes
-miRNA genes
-siRNA
-IncRNA genes
-most snRNA
5What does poly 2 require to function?
-general transcription factors
6Role of TFIID? and structure
-multisubunit complex that forms pre initation complex
-made up of TAFS (transcription assoication factors)
-major component is TBP (tata binding protein)
7Role of TBP, where is it found?
(tata binding protein)
-major part of TF11D
-is associates with tata binding protein in euk
8where are tata boxes ussually located? (bp wise)
-30 to -25 bp
9after TF11D binds with tata box, what transcription factor is recruited? why?
-TF11B is recruited
10after TF11B is recruited what happens next?
-TF11E
-TF11F
-TF11H
and
RNA poly 2
11what must be present to enable enhancer and pre-initiation complex interaction?
-mediator must bind on pre-initator complex.
12What binds to enhancer?
histone remodeling complexes
13What remodeling/modifying components must be included in pre-initiation complex?
-chromatin remodeling complex
-histone modifying enzyme
14What are three major regions in transcription region of euk dna?
tata box
INR (initation transcription site)
DPE (downstream promoter elements)
15Role of TF11F?
-helps stabalize RNA poly with TBP and TF11B
16Role of TF11E?
attracts and regulates TH11H
18what are enhancer regions? characteristics?
-dna sequence where proteins bind onto
-specified to given promoter (gene)
-activity may depend on distance and orientation
-mostly cis-operating element
19What process enables interaction between enhancer and mediatior (pre-initation complex)?
-DNA looping (allows TF's bound to distant enhancer to interact with proteins of RNA poly/mediator complex at promoter)
20what protein helps with proper DNA looping formation?
-Cohesin (stablizes location of enhances with promoter)
21What directs cohesin?
-CtCF
22purpose of elongation factors?
-forms a complex with RNA poly 11
-unwraps DNA and retains histones for nucleosome reassembly
23What does CTD stand for? Where is it?
C-terminal domain of RNA poly 11
24Purpose of CTD?
-C-terminal tail
-docking station for other proteins
-codes for phosphorylation (determines what can bind there)
25Role caping proteins in regulation?
7-methylguanosine added to 5 prime end of primary transcipt
cap only happens once
26overview steps of rna processing?
-transcription
-5 prime capping
-RNA splicing
-3 prime polyadenylation