Eukaryotic Transcription

-transcribes rRNA genes
-located in nucleolus

5.8 S
18S
28 S

-transcribes tRNA genes
-rRNA genes
-some snRNA
-genes for other small rna's

-transcribes all protein coding genes
-snoRNA genes
-miRNA genes
-siRNA
-IncRNA genes
-most snRNA

-general transcription factors

-multisubunit complex that forms pre initation complex
-made up of TAFS (transcription assoication factors)
-major component is TBP (tata binding protein)

(tata binding protein)
-major part of TF11D
-is associates with tata binding protein in euk

-30 to -25 bp

-TF11B is recruited

-TF11E
-TF11F
-TF11H
and
RNA poly 2

-mediator must bind on pre-initator complex.

histone remodeling complexes

-chromatin remodeling complex
-histone modifying enzyme

tata box
INR (initation transcription site)
DPE (downstream promoter elements)

-helps stabalize RNA poly with TBP and TF11B

attracts and regulates TH11H

helicase

-dna sequence where proteins bind onto
-specified to given promoter (gene)
-activity may depend on distance and orientation
-mostly cis-operating element

-DNA looping (allows TF's bound to distant enhancer to interact with proteins of RNA poly/mediator complex at promoter)

-Cohesin (stablizes location of enhances with promoter)

-CtCF

-forms a complex with RNA poly 11
-unwraps DNA and retains histones for nucleosome reassembly

C-terminal domain of RNA poly 11

-C-terminal tail
-docking station for other proteins
-codes for phosphorylation (determines what can bind there)

7-methylguanosine added to 5 prime end of primary transcipt
cap only happens once

-transcription
-5 prime capping
-RNA splicing
-3 prime polyadenylation