Molecular Biology: DNA and RNA Synthesis

are responsible for folding DNA into nucleosomes. (Core Histones)

is bound to the DNA where the double helix enters and leaves the nucleosome core.
(Linker Histone)

H3-H4 tetramers, 2 dimers of H2A-H2B

high GC content

1. Initation
2. elongation
3. temination

template, UTP, metal ions

- Sigma searches for and binds to promoter sequences, initiation sites for RNA synthesis
- DNA double helix is unwound

Select the ribonucleoside triphosphate that corresponds to the DNA template and catalyze them formation of a phosphodiester linkage

- holoenzyme helps the polymerase locate promoter sites
- catalytic

TTGATA - TATAAT
TTGACA

CTGACG - TACTGT
TTGACA - TATAAT

- σ4 recognizes the -35 region, σ2 recognizes the -10 region

- OMP
- Oxidative stress dephosphorylates RsbV to release σ

a purine

Simplest stop signal is a segment of palindromic DNA (rho-independent)