1Main Stages
Electron Transport Chain (ETC) and Chemiosmosis.
2Electron Transport Chain
Series of proteins that transfer electrons from NADH/FADH₂ to oxygen.
3Proton Gradient
H⁺ concentration difference across inner membrane created by ETC.
4ATP Synthase
Enzyme that uses proton flow to synthesize ATP from ADP + Pi.
5Final Electron Acceptor
Oxygen (O₂) forming water after accepting electrons.
6Chemiosmotic Gradient
Potential energy stored in proton gradient used for ATP production.
7Total ATP Yield per Glucose
~34 ATP from oxidative phosphorylation (≈36 total including glycolysis & CAC).
8Uncouplers
Substances that dissipate proton gradient, reducing ATP output and producing heat.
9Cyanide/CO Effect
Block ETC by binding to complex IV → halts ATP production and cell death.