1Aminoacyl-tRNA Synthetases
tRNAs with an amino acid are charged
2Ribosomes
bind mRNA and identify the Start codon for translation, facilitate complementary base-pairing between mRNA codons and tRNA anticodons, catalyze peptide bond formation
3EF-G
fits into the A site displacing the peptidyl-tRNA, hydrolyzes GTP to GDP causing the ribosome to change shape and shift over one position
4• eIF1A
blocks binding to the A site and premature association of the large subunit
5eIF2 & eIF5B
bring the charged tRNA to the P site
6eIF3
blocks association of large subunit
7eIF4E
binds to the 5' cap of mRNA
8eIF4A
unwinds parts in the 5' untranslated region that may have paired up with itself, uses ATP to promote the ribosome to scan for the Kozak sequence and authentic start codon