1Biochemical test is produced because diff. microbes produce diff. enzymes
2CATALASE TEST
A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false
1. Place a drop of 3% H2O2 to a glass slide and get a loopful of bacterial colony from culture and mix.
2. Catalase activity is indicated by change of color from yellow to blue.
3COAGULASE
A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false
1. Drop H2O2 on a clear glass slide and prepare a heavy, even suspension of unknown Enterobacter.
2. Place a drop of reconstituted coagulase plasma opposite to the drop of bacterial suspension. Thoroughly mix together.
4COAGULASE
A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false
3. Title the slide back and forth, observing for the formation of granular precipitate or white clumps.
Observe10 seconds for clotting of the mixture,
5OXIDASE
A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false
1. To a sterile test tube, place a drop of Kovac’s reagent and get a loopful of bacterial colony from your culture and mix.
2. Observe for bubbling.
6OXIDASE
Which statment is correct?
3. Observe for lavender to dark purple result
3. Observe for red to pinkish result
7WASTA MANAGEMENT
Disinfect with 10% hypochlorite. Reuse after drying. Flame until it becomes red hot before using.
- COTTON BALL PENCIL SHAVINGS
- PIPETTES/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE GLOVES AND MASK
- DISPOSABLE INOCULATING LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE OF E.COLI OF AND OF S.AUREUS
8WASTE MANAGEMENT
Disinfect with 10% hypochlorite(at least one hour); Reuse until dry.
- COTTON BALL PENCIL SHAVINGS
- PIPETTES/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DIPOSABLE GLOVES AND MASK
- DISPOSABLE INOCULATING LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE OF E.COLI AND S. AUREUS
9WASTE MANAGEMENT
Disinfect in lysol; place in standard yellow plastic bag (labeled infectious waste). thorw innthe infectious waste bin
- COTTON BALL/PENCIL SHAVINGS
- PIPETTE/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE inoculating loop and needle
- DISPOSABLE INOCULATIONG LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE OF E.COLI AND S.AUREUS
10WASTE MANAGEMENT
Dispose in a plastic bag container, throw in the infectious waste bin
- COTTON BALL/PENCIL SHAVING
- PIPETTE/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE GLOVES AND MASK
- DISPOASBLE INOCULATING LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE OF E.COLI AND S.AUREUS
11WASTE MANAGEMENT
Disinfect by burning the tips and Dispos in a plastic bag container, throw in the infectious waste bin
- COTTON BALL/PENCIL SHAVINGS
- PIPETTE/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE GLOVES AND MASK
- DISPOSABLE INOCULATING LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE E.COLI AND S.AUREUS
12WASTE MANAGEMENT
Disinfect in Lysol(at least 2 hours); place the culture in standard yellow plastic bag(labeled infectious waste), throw in the infectious waste bin
- COTTON BALL/PENCIL SHAVINGS
- PIPETTE/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE GLOVES AND MASK
- DISPOSABLE INOCULATING LOOP AND NEEDLE
- PREPARED USED IN CULTURE MEDIUM
- CULTURE E.COLI AND S.AUREUS
13WASTE MANAGEMENT
Disinfect in Lysol(at least 2hrs); place the culture in standard yellow plastic bag(labeled infectious waste), throw in the infectious waste bin
- COTTON BALL/PENCIL SHAVINGS
- PIPETTES/DROPPERS
- WET COTTON BALL/USED THROAT SWAB APPLICATOR/USED TONGUE DEPRESSOR
- DISPOSABLE GLOVES AND MASK
- DISPOSABLE INOCULATING LOOP AND NEEDLE
- PREPARED USED CULTURE MEDIUM
- CULTURE OF E.COLI AND S.AUREUS