1UTA microbiology lab classrooms are Biosafety Level 2. Which of the following are requirements for this safety level? (select all that apply)
All persons wear PPE (gloves, lab coat, and goggles))
Sink and eye wash stations are present.
Restricted access to authorized persons only.
Access to an autoclave sterilizer.
2To clean a biohazard spill (such as spilling a tube of E. coli) you must cover the spill with paper towels and soak with Lysol for 1-2 minutes.
True
3It is okay to wear sandals in the micro lab classroom if you know you won't be working with harmful bacteria.
False
4Where is the appropriate place to dispose a used petri dish?
Red biohazard bin
5When an autoclave reaches the "sterilization phase" this means that the temperature i ___C and the pressure is ___psi .
121, 15 psi
6It is best to invert and store petri dishes so that condensation does not drip and contaminate the agar.
True!
7Knowing the density of water at room temperature, how much would 350 µl of water weigh in grams?
0.35 g
8The flame from a Bunsen burner kills all the microbes and sterilizes the wire loop. Why is this important to do before working with a sample?
Avoid contamination
9What part of a microscope does the number "3" in the image above indicate?
objective lens
10The use of immersion oil in brightfield microscopy is to improve:
numerical aperture
11Examine the disk diffusion assay below. Which disinfectant works the LEAST?
F
12What does a "zone of inhibition" represent in a disk diffusion assay?
the amount of inhibited microbial growth
13Which is the countable range used in the viable cell count method?
30-300 CFUs
14Examine the serial dilution illustrated below. What is the correct dilution tube for obtaining the most accurate viable cell count?
1:10,000 ( 50 counted colonies)
15In the viable cell count method, which type of inoculation is used?
spread plate inoculation
16In the disk diffusion and Kirby Bauer exercise, which two bacteria will you be using? (select all that apply)
Staphylococcus aureus and Escherichia coli
17In the preservative efficacy test (PET), which technique will you be using to assess the efficacy of a preservative?
Viable cell count method
18The Analytical Profile Index (API) 20E test is useful for:
identification of enteric, Gram negative bacteria.
19Anabolism reactions are exergonic pathways that break down complex molecules into simpler ones and release energy in the form of ATP.
False!
20The Kirby Bauer assay cannot distinguish between bacteriocidal and bacteriostatic activity of an antibiotic.
True!!
21The Gram stain is an example of:
differential staining
22Match each step of the Gram stain with the correct description.
EASYYY
Heat fix bacteria , primary stain (crystal violet), Mordant (iodine), Decolorizor (ethanol), Counter stain (Safranin) always rinse in between
23Which could lead to a false negative in the Gram stain technique?
Leaving on decolorizer too long.
24Stains must never be poured down the sink.
True
AFFF
25The Acid Fast staining technique is useful in diagnosing which type of infection?
Mycobacterium spp.
26What serves as the primary stain in the Acid Fast technique?
carbol fuschin
27What serves as the "mordant" in the Endospore staining technique?
steam
28Endospores are considered metabolically active while vegetative cells are metabolically inactive.
Falsey false
29All bacteria are capable of producing endospores under stressful conditions.
Negativeeee soldier
30Examine the Gram stain image above. Which is NOT a conclusion or observation you can make? (picture of purple coccus bacteria and bacillus pink bacteria at 10 micrometers)
This specimen contains Acid-Fast bacteria.
31Examine the serial dilution above. What is the CFU/ml of the original container?
5x10^6
32What is the FDA minimum requirement for a preservative to be considered effective at 14 days?
1 log reduction of CFU/ml from the initial (Day 1) concentration.
33What is the primary function of fermentation?
Oxidation of NADH to NAD+
34In alcohol fermentation, which are common byproducts? (select all that apply)
ethanol, citric acid, CO2
35Which generates the larges amount of ATP energy for the cell?
Electron transport chain
36Biofilm communities can be made of several different bacterial species.
very true
37Which is not an explanation for how biofilms resist antibiotics?
Biofilms can contain yeast that help protect other microbes against antibiotics.
38Appendages (fimbriae, pilus, and flagella) help establish the formation of a mature biofilm.
Yup, True
39Which occurs first in biofilm development?
Planktonic cells obtain reversible attachment to a substrate.
40Which types of microbes are responsible for the creation of ginger beer? (select all that apply)
bacteria, yeast
41Which is used to create the modern "tree of life"?
16s ribosomal RNA gene
42Which is not a modern day domain?
Prokarya
43In this lab, you will be extracting all genomic content of your bacterial cells.
yeah for lab 8 we did bacterial dna extraction
44Which is the first process that occurs during DNA extraction?
cell lysis
45Both the API test and sequencing are used together to get the most accurate species identification.
yessir true
46Which stain will be used in the biofilm assay?
crystal violet
47Data show there is a higher occurrence of antibiotic resistance among biofilm sessile cells versus planktonic cells.
truetruetrue
48According to Magill et al. 2011, which microorganism is most commonly associated with HAIs?
Staphylococcus Aureus