CELL BIO CH 9

1, 4, 3, and 2

you can see individual electrons

you can see organelles or cells

you can see componenets of organells

good for dead cells

dark field microsopy, and phase contrast , differential contrast

2, 4, 3, 1

fixes tissue cuts onto a slide and forms covalent bonds

greater resolution

greater brightness

protiems derived from animals with naturally occurring bioluminescence

can track promoter sequences and gene expression

normally strong agents containting one or more metals

a fluroscent marker that binds to protien

amplified fluroscefore

the process involving primary and secondary antibodies

detailed selectional views provided of an intact internal structure limited by 150um

point focused is fuzzy

a dichrotic mirror and a laser limited by 40um

infared light

colocalize and protien imteraxtion fret

Fluroscence resonance excitation good for following protien interactiins

fluroscenc recovery after photobleaching, good fo seeing protien or lipids diffusing around the cell

boosts light microscopy, superesolution

blocks out light not in focus

use long wavelnegths to pemetrate into a tissue, less risk of tissue damage

strategy for superofularion bleaches arojmd to see a poimt

Total refraction imternal fluroscence

specimin swells so u can use a normal microscope

wavelength is very tiny, resolution is 0.06 nm and its done in a vaccumn to prevent electrons scattering , done w magnesrs(projector lens)

localizes and creates contrast by binding to the metal

transmission electron tomography, take many images to create 3D structure

best for fine structures of areas of imteeest

can make a fluorescent microscopy and make struxture

specific onlg to DNA and RNA and uses a probe to check gene expression