1Where does post transcriptional modification take place inside the nucleus?
in subnuclear aggregates
2What is structurally distinct about RNA's that are targeted for degradation by the RNA exosome?
-uncapped rna
-un-polyadenylated rna's
poly ubiquinated by
3what is structurally distinct about mature mrna's as they are leaving? what interaction does this facilitate
have cap-binding complex
facilitates interaction of mrna with nuclear export receptors
4where are mrna complexes exported to from the nucleus?
-exported to the cytoplasm
5what is the purpose of non-sense mediated RNA decay process?
-process to degrade damaged or incorrectly spliced transcripts (may have early termination sequence)
6What are essential components of the subnuclear aggregates?
-aggregation factor
-chromosomes in association
-scaffold proteins
7what is RBM7?
-protein complex in the nucleus that guides nuclear RNA's to exosome
-helps recruit helicase to unwind the rna allowing ease of accessibility to the exosome
8What should be removed from the mrna by ribosome in effective translation?
-the EJC (exon junction complexes) should be removed
9What happens if the EJC can't be removed from mrna during translation? please be specific with the exosome decay
-ribosome remains attached
-it is tagged with Upf proteins
-mrna is degraded (decapping, deadenylation exosome mediated decay 5' to 3')
10what region on the mRNA regulates ribsome binding?
-untranslation regions (UTR's)
11What are PABP's? Role?
Poly-Adenine Binding Proteins
stabilizes mRNA, assists with translation initiation and termination, helps with ribosome recycling
12what must be removed from a protein during processing?
-terminal methionine is removed (most of the time)
13Role of HSP70?
protein that binds to exposed hydrophobic regions of freshly translated protein to prevent misfolding and protein aggregation
14what does monoubiquitylation do?
aids in histone regulation (chromatin changes)
-assists with nuclear export
-assist with endocytosis
-assist with membrane trafficking
15What are the two routes of poly-uniquitination, be specific please.
K48 leads to degradation
K63 leads to recruitment of helper proteins and repair
16you're killing it honey
keep going :)