1ISE Membrane for Sodium
Glass aluminum silicate
2Routine method for Sodium
Ion-Selective Electrode
3Colorimetry for Sodium
Albanese-Lein
4Reagent for Sodium
Sodium uranyl zinc acetate
5End color of Sodium using the reagent
Yellow
6Reference method for Sodium
FEP + ISE
7Routine Method for Potassium
Ion-Selective Electrode
8ISE Membrane for Potassium
Valinomycin gel (Ionophore)
9Colorimetry for Potassium
Lockhead and Purcell
10Reagent for Potassium
Sodium cobaltinitrite
11End color of Potassium using reagent
Blue
12Reference method for Potassium
FEP + ISE + AAS
13Mercurimetric Titration for Chloride (SERUM)
Schales and Schales
14Mercurimetric Titration for Chloride (URINE)
Fantus
15Reagent for Chloride (Mercurimetric Titration)
Mercuric nitrate
16Indicator for Chloride (Mercurimetric Titration)
Diphenylcarbazone
17Color for the indicator of chloride
Blue-violet
182 Spectrophotometric Assays for Chloride
a. Mercuric Thiocyanate (White Horn) Titration Method
b. Ferric Perchlorate
19end product color for chloride (Mercuric Thiocyanate Titration Method)
(+) reddish complex end product
20Most commonly used method for Chloride
Ion-Selective Electrode
21IFCC-recommended reference method for chloride
Coulometric Titration with Amperometric end point
22Reference Method for Chloride
Ion Chromatography or Inductively Coupled Plasma Mass Spectrometry Method
232 Precipitation and Redox Titration
a. Clark Collip Precipitation
b. Ferro ham Chloranilic Acid Precipitation
24Precipitation and Redox Titration for
Total Calcium
25End product and color of Clark Collip Precipitation
Oxalic acid (purple color)
26Ferro ham Chloranilic Acid Precipitation end product and color
Chloranilic acid (purple color)
27Clark Collip Precipitation (Electrolyte)
Calcium
28Ferro ham Chloranilic Acid Precipitation (Electrolyte)
Calcium
29Test to detect Calcium in urine
Sulkowitch
30Sulkowitch Test (Electrolyte)
Calcium
31Colorimetric Methods for Total Calcium
a. Ortho-Cresolpthalein Complexone (OCC) Dye
b. Arzenazo III Dye
32End color reaction of Ortho-Cresolpthalein Complexone (OCC) Dye
Red/purple in alkaline solution
33Ortho-Cresolpthalein Complexone (OCC) Dye (Electrolyte)
Calcium
34End color reaction of Arzenazo III Dye
Violet colored complex
35Arzenazo III Dye (Electrolyte)
Calcium
36Reference Method for Calcium
AAS (Atomic Absoption Spectrophotometry)
37Commonly used method to measure serum inorganic PHOSPHATE
Fiske Subbarow or Phosphomolybdate Method
38Phosphomolybdate Method (other name)
Fiske Subbarow
39Most common reducing agent
Pictol (Amino Napthol Sulfonic Acid)
40Product of Pictol
Ammonium-molybdate complex (unstable)
41Colorimetric Methods for Magnesium
a. Calgamite
b. Formazan Dye
c. Methylthymol Blue
42Most commonly used method for MAGNESIUM
Calgamite
43Calgamite end color
(+) reddish-violet (alkaline pH at 530nm)
44Dye-Lake Method (Dye)
Titan Yellow Dye or (Clayton Yellow or Thiazole Yellow)
45Reference Method for Magnesium
AAS (Atomic Absoption Spectrophotometry)
46Measures free Magnesium
Ion Selective Electrode (neutral ionophores)
47ISE Membrane for Magnesium
Neutral carrier polymer
48Sample for Bicarbonate
Blood (anaerobically collected)
49Sample Precaution for Bicarbonate
If the sample is left UNCAPPED before analysis, levels decrease by 6 mmol/L/hour
50BICARBONATE collection
CAPPED
51pH if uncapped for Bicarbonate
High pH
52Methods for Bicarbonate
1. Ion-selective electrode (using the pCO electrode)
2. Enzymatic (Phosphoenolpyruvate carboxylase and dehydrogenase using alkaline reagent)
53FEP colors (Potassium)
Violet
54FEP colors (Sodium)
Yellow
55FEP colors (Copper)
Green
56FEP colors (Lithium)
Pink to Red
57FEP colors (Strontium)
Red
58FEP colors (Calcium)
Orange
59FEP colors (Barium)
Lime Green
61Specialized membrane (Na)
Hydrated silicate glass
62Specialized membrane (K)
Valinomycin
63Specialized membrane (Cl)
Silver chloride/ Silver sulfate
64Specialized membrane (Ca)
Polyvinyl chloride