1Identify the main component of the red blood cell, comprising approximately 95% of its cytoplasmic content
Hemoglobin
2List the other names for Hemoglobin as written in the handwritten notes
Respiratory pigment or chromoprotein
3State the concentration of Hemoglobin within RBCs
approximately 34 g/dL
4Identify the approximate molecular weight of Hemoglobin
64,000 Daltons
5Identify the individual who identified Hemoglobin as the respiratory protein in 1862
Felix Seyler
6Specify the percentage of cytoplasmic hemoglobin produced before the nucleus is extruded
65%
7Identify the cell stage where the remaining 35% of hemoglobin is synthesized
early reticulocyte
8Identify the single most common complex organic molecule in vertebrates
Hemoglobin
9State the specific amount of O_2 that 1 gram of hemoglobin can carry
1.34 mL
10State the constant amount of iron (Fe) that 1 gram of hemoglobin carries
3.47 mg
11Enumerate the 4 different constituents of a complete adult hemoglobin molecule
Two sets of two different polypeptide chains (globin), Four molecules of protoporphyrin IX, Four iron atoms (Fe^{2+}), and One 2,3-BPG molecule
12State the number of amino acids in an Alpha globin chain
141
13State the number of amino acids in a Zeta globin chain
141
14State the number of amino acids in Beta, Delta, Epsilon, and Gamma globin chains
146
15Identify the specific amino acid positions for Gamma A and Gamma G chains
Position 136 (Alanine for A, Glycine for G)
16List the three primary functions of Hemoglobin
Delivery of oxygen to the tissues, To carry waste product CO_2 away from lungs, and Buffering, inactivation and transport of NO (nitric oxide)
17Identify the reference method and best/most widely used method for hemoglobin determination
Cyanmethemoglobin (HiCN) Method
18Describe the chemical principleeaction of the HiCN method
Hemoglobin (Fe^{2+}) + K_3Fe(CN)_6 ightarrow methemoglobin (Fe^{3+}) + KCN ightarrow cyanmethemoglobin
19Identify the two major components of Drabkin's reagent
Potassium ferricyanide (K_3Fe(CN)_6) and Potassium cyanide (KCN)
20Identify the component of Drabkin's reagent that converts iron from the ferrous to ferric state
Potassium ferricyanide
21Identify the wavelength at which HiCN is measured
540 nm
22Name the type of hemoglobin that cannot be converted and measured by the HiCN method
Sulfhemoglobin
23State the time and temperature required for full conversion of hemoglobin to cyanmethemoglobin
10 minutes at room temperature
24Identify the correction for a falsely elevated Hb due to a high WBC and platelet count (>20 \times 10^9/L)
Centrifuge reagent-sample solution, then the supernatant is measured
25Identify the correction for a falsely elevated Hb due to Lipemia
Add 0.01 mL of the patient's plasma to 5 mL of the cyanmethemoglobin reagent and use this solution as the reagent blank
26Identify the correction for a falsely elevated Hb due to cells containing Hb S and Hb C
Make a 1:2 dilution with distilled water and multiply results by 2
27Identify the substance added to the reagent to prevent precipitation caused by abnormal globulins
0.1 gram of potassium carbonate
28Identify the chemical salt found in modified commercially available HiCN reagents that prevents globulin precipitation
KH_2PO_4
29Identify the storage requirement for Cyanmethemoglobin reagent due to its light sensitivity
Brown bottle or a dark place
30Identify the automated technique that transforms hemoglobin into SLS-methemoglobin without producing toxic wastes
Sodium lauryl sulfate (SLS)
31Identify the commercially available handheld system used to measure hemoglobin concentration
HemoCue
32Specify the two wavelengths used in the HemoCue photometric measurement
570 nm and 880 nm
33Identify the final product measured by the HemoCue system
azidemethemoglobin