Gram Positive Cocci (GPC) - Staphylococcus species
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1
Staphylococcus aureus — Biochemicals (7)
CAT (+),
COAG (+),
Novobiocin (S) >16mm,
MAN (+),
Bacitracin (R),
Microcidase (-),
Lysostaphin lysis (+)
COAG (+),
Novobiocin (S) >16mm,
MAN (+),
Bacitracin (R),
Microcidase (-),
Lysostaphin lysis (+)
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Staphylococcus aureus — Plates
Growth on BAP,
MS (yellow), &
CHROMagar S. aureus (mauve)
MS (yellow), &
CHROMagar S. aureus (mauve)
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Staphylococcus aureus — Colony morphology
Dense, large, smooth, buttery, golden/yellow, β-hemolytic colonies
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Staphylococcus aureus — Gram stain
GPCCI
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Staphylococcus aureus — Source/associations
Pathogenic anywhere in the body
Skin infections to systemic illnesses;
Staphylococcal scalded skin syndrome, toxic shock syndrome via TSST-1, & food poisoning
Skin infections to systemic illnesses;
Staphylococcal scalded skin syndrome, toxic shock syndrome via TSST-1, & food poisoning
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S. saprophyticus — Biochemicals (4)
CAT (+),
COAG (-),
Novobiocin (R) ≤16mm,
MAN (v)
COAG (-),
Novobiocin (R) ≤16mm,
MAN (v)
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S. saprophyticus — Plates (1)
Growth on BAP
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S. saprophyticus — Colony morphology
Large, flat, smooth, opaque, white colonies
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S. saprophyticus — Gram stain
GPCCI
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S. saprophyticus — Source/associations
Acute UTI in young sexually active females (urethritis, cystitis, pyelonephritis) & IUD
Urethritis, catheter-associated UTI, & prostatitis in males
Urethritis, catheter-associated UTI, & prostatitis in males
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S. epidermidis — Biochemicals (4)
CAT (+),
COAG (-),
Novobiocin (S),
MAN (-)
COAG (-),
Novobiocin (S),
MAN (-)
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S. epidermidis — Plates (1)
Growth on BAP
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S. epidermidis — Colony morphology
Large, smooth, white colonies
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S. epidermidis — Gram stain
GPCCI
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S. epidermidis — Source/associations
Most common agent of prosthetic valve endocarditis
Most common agent of bacteremia in immunocompromised patients (IV catheters, CSF shunts, CSF catheters)
Most common agent of bacteremia in immunocompromised patients (IV catheters, CSF shunts, CSF catheters)
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S. lugdunensis — Biochemicals
CAT (+),
Slide COAG (v),
Tube COAG (-),
PYR (+),
ODC (+)
Slide COAG (v),
Tube COAG (-),
PYR (+),
ODC (+)
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S. lugdunensis — Plates
Growth on BAP
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S. lugdunensis — Colony morphology
β-hemolytic white colonies
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S. lugdunensis — Gram stain
GPCCI
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S. lugdunensis — Source/associations
More pathogenic coag neg staph
Can cause endocarditis, bacteremia, & abscesses
Can cause endocarditis, bacteremia, & abscesses