Gram Positive Cocci (GPC) - Staphylococcus species

CAT (+),
COAG (+),
Novobiocin (S) >16mm,
MAN (+),
Bacitracin (R),
Microcidase (-),
Lysostaphin lysis (+)

Growth on BAP,
MS (yellow), &
CHROMagar S. aureus (mauve)

Dense, large, smooth, buttery, golden/yellow, β-hemolytic colonies

GPCCI

Pathogenic anywhere in the body

Skin infections to systemic illnesses;

Staphylococcal scalded skin syndrome, toxic shock syndrome via TSST-1, & food poisoning

CAT (+),
COAG (-),
Novobiocin (R) ≤16mm,
MAN (v)

Growth on BAP

Large, flat, smooth, opaque, white colonies

GPCCI

Acute UTI in young sexually active females (urethritis, cystitis, pyelonephritis) & IUD
Urethritis, catheter-associated UTI, & prostatitis in males

CAT (+),
COAG (-),
Novobiocin (S),
MAN (-)

Growth on BAP

Large, smooth, white colonies

GPCCI

Most common agent of prosthetic valve endocarditis

Most common agent of bacteremia in immunocompromised patients (IV catheters, CSF shunts, CSF catheters)

CAT (+),
Slide COAG (v),
Tube COAG (-),
PYR (+),
ODC (+)

Growth on BAP

β-hemolytic white colonies

GPCCI

More pathogenic coag neg staph
Can cause endocarditis, bacteremia, & abscesses