Topics 9 & 10: Mitosis and Meiosis

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1
Chromatography
Technique that separates a complex mixture into its components based on solubility (polarity).
2
Pigment Polarity
Carotene = least polar; Xanthophyll (zeaxanthin) = more polar; Chlorophyll b = most polar.
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Absorption Spectra
Graph showing how much light a pigment absorbs at various wavelengths; pigments together capture more of the light spectrum.
4
Photosynthesis (2nd stage)
CO₂ fixation stores energy in carbohydrates (glucose, starch). Lugol's iodine stains starch to show where photosynthesis occurs.
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Cell Cycle
The process cells use to grow, replicate DNA, and divide into two identical daughter cells.
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Interphase
Includes G1, S, and G2 phases; DNA exists as uncoiled chromatin.
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G1 Phase
Cell grows and carries out normal functions.
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S Phase
DNA replication occurs.
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G2 Phase
Cell prepares for mitosis.
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M Phase
Includes mitosis (nuclear division/chromosome separation) and cytokinesis (cytoplasmic division).
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G0 Phase
Resting state for non-dividing cells (e.g., muscle, nerve cells).
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Cell Cycle Checkpoints
G1 Checkpoint: Cell size, nutrients, DNA damage, growth factors; G2 Checkpoint: DNA replication completion; M Checkpoint: Chromosomes aligned at metaphase plate.
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Mitosis
Division of the nucleus producing two genetically identical daughter cells.
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PMAT
Prophase, Metaphase, Anaphase, Telophase — main stages of mitosis.
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Prophase
Nuclear membrane disappears; chromosomes condense; spindle forms.
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Metaphase
Chromosomes (sister chromatids) line up at the metaphase plate.
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Anaphase
Sister chromatids separate and move toward opposite poles.
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Telophase
Nuclear membranes reform; spindle breaks down; chromosomes uncoil.
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Cytokinesis
Division of the cytoplasm; forms two separate daughter cells.
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Chromatin
Uncoiled DNA + proteins (during interphase).
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Chromosome
Condensed DNA + proteins (during mitosis).
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Genome
Complete set of an organism's genetic material.
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Sister Chromatids
Identical copies of a duplicated chromosome joined at the centromere.
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Centromere
Region where sister chromatids attach.
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Centrosome
Microtubule-organizing center of the cell.
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Centriole
Cylinder-shaped structure composed of microtubule triplets.
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Ploidy (n)
Number of sets of chromosomes in a cell.
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Diploid (2n)
Two copies of each chromosome (e.g., humans 2n = 46).
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Haploid (n)
One copy of each chromosome (e.g., gametes, n = 23).
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Homologous Chromosomes
Pair of chromosomes with the same genes but possibly different alleles (one from each parent).
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Meiosis
Cell division producing four haploid gametes; genetic diversity increased.
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Meiosis I
Separates homologous chromosomes, reducing ploidy (2n → n).
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Meiosis II
Separates sister chromatids, similar to mitosis.
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Mendel's First Law (Law of Segregation)
Two alleles for a trait separate during gamete formation; each gamete gets one allele.
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Mendel's Second Law (Independent Assortment)
Alleles of unlinked genes assort independently during gamete formation.
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Locus
Specific location of a gene on a chromosome.
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Allele
Variant form of a gene (e.g., A vs. a).
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Homozygous
Two identical alleles (AA or aa).
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Heterozygous
Two different alleles (Aa).
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Dominant vs. Recessive
Dominant allele: Expressed (A); Recessive allele: Hidden unless homozygous (a).
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Punnett Square
Tool to predict offspring genotypes and phenotypes.
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Dihybrid Cross
Cross tracking two traits; shows independent assortment of alleles.
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During interphase...
DNA is uncoiled and exists as chromatin (DNA + histone proteins).
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During mitosis...
DNA is highly coiled into chromosomes.
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