1Explain how the structure of a chloroplast shows adaptations to its functions. Large numbers of thylakoids:
More thylakoids means more chlorophyll/other pigments, so more light can be absorbed. This leads to more light dependent reaction, more ATP and NADPH created
2Explain how the structure of a chloroplast shows adaptations to its functions.Stroma filled with enzymes.
More enzymes in the stroma increases the likelihood that molecules will come in contact with the enxzmes and react; this means more reactions of the Calvin Cycle can take place.
3Where on the graph of the action spectrum of photosynthesis is the peak?
475 nm and 750 nm
4Explain the relationship between the abosption spectrum for chlorophyll and action spectrum of photosynthesis for green plants.
The absoption spectrm for chlorophyll has a smaller range thn the action spectrum for green plant. Green plants contain pigments other than chlorophyll called accessory pigments. These pigments absorb different wavelngths of light than chlorophyll, so their presence increases teh toal amount of light tht is absorbed.
5What are the first step in light dependent reactions?
1) water is oxidized to create oxygen and replace teh electrons lost. oxygen is released to the environment
6What are the second step in light dependent reactions?
As electrons pass along the electron transport chain, H+ gets actively transported in ot the interior of the thylakoid.
7What are the third step in light dependent reactions?
Electrons are re energized when the chlorophyll in photosystem 1 absorbs sublight. Eventually the electrons are passed to NADP+ reductase which reduces to NADPH. The product travels to the stroma for the Calvin Cycle.
8What are the fourth step in light dependent reactions?
Due to the high concentration of H+ ions in the thylakoid interior, there is diffusion out through ATP synthase. The movement of H+ ions out of the thylacoid create ATP. ATP moves to the stroma for the Calvin Cycle
9What is the role of water in the light-dependent reactions
Water is used to replace electrons in Photosystem II.
10What is produced from water? What is the process called that splits the water?
This process is called photolysis. in addition to electrons, it also releases H+ and oxygen
11Where in the chloroplast does the light-dependent reaction occue?
thylakoid
12What occurs when light is absorbed by the chlorophull in photosystem II?
The light energy excites electrons. The excited electrons are passed from Photosystem II to the ETC and their energy pumps H+ into the Thylakoid.
13Which molecules are oxidized in the light dependent reaction
Water
14Which molecule are reduced in light dependent reaction
NADP+
15Which two molecules travel to the light independent reaction ?
nadph and atp
16Describe how ATP synthase uses chemiosmosis to phosphorylate ATP
A high concentration of H+ is created inside of the thylakoid. when it is permitted to flow back down the concentrations gradients (high to low) through ATP synthase, the release of potential energy powers the phosphorylation of ADP thus creating ATP
17What is chemiosmosis
the flow of ions donwn their electrochmical gradient
18What is phosphorylation
addition of phosphate group
19oxidation is ____ of electrons and hydrogen (gain of oxygen). reduction is ___ of electrons and hydrogen (loss of oxygen)
loss; gain
20What is the name of 5 carbon sugar in the Calvin Cycle?
RuBP
21Which tech. advance enabled Calvin to perform his lollipop experiment on the light - independent reactions of photosyntesis in 1949
Methods for tracing radioactive carbon incorporated in molecules produced by the Alga Chlorella
22Identify two ways that the emergence of photosythesis aftected the Earth atmosphere, oceans, or rock deposition in Earth's early history
Increased amount of oxygen in the atmosphere, decreased the amount of grenhouse gases. this led to glaciation of the earth and deposition of iron oxide (rust) on the ocean flook (present today in rock striations) and a mass extinction event
23What are some factors that affect the rate of photosynthesis?
light intensity, carbon dioxide concentration, temperature
24how does temperature changes enzymatic activity
(makes it kineetic energy of molecules or denatures it
25what does limiting factor mean
the liimiting factor is the factor that is determing the rate of photosynthesis. if this factor increases, the rate of photosynthesis increases. if the factor is not limiting, an increase will not increase the rate of photosynthesis.
26in graphs, as light intensity and CO2 amount increase, they are no longer limiting factors, therefore, why does the graph level out?
the point where the graph levels is called the satuation point. at this point all enzymes/photosystems are in use and there cannot operate any quicker
27what is photosynthesis
a chemical reaction that converts light energy into chemical energy (glucose)
28what is an absorbance spectrum
it shows the percentage of light absorbed at each wavelength for three different pigments found in plants.
29chlorophyll a, what is it
the most abundant pigment in plants which is what makes plant appear gree
30what colors does chlorophyll a abosrb most
reds and violets
31why is it we see green when we look at a plant
green wavelengths of light are reflected by these pigments
32what is the other pigments other than chlorophyll a clled?
accessory pigments
33why is accessory pigments important
having accessory pigments means plants can absorb more wavelengths of light than they could with chlorophyll a alone
34how many chloroplasts in each plant cell?
100
35where is clorphyll located in a chlorplast?
in the membranes of thylakoids
36photosynthsis has many steps, but organized into two steps, what are they?
the light dependent reacion and light independent reaction
37what is light independent reaction also caleld
calvin cycle
38what is the reaction for photosynthesis
6CO2 + 6H2O --> C6H12O6+6O2
39Why is the light dependent reaction named that way?
the reaction depends on light to happen
40Why is the light independent reaction named that way?
the reaction doesn't need light to happen
41What product essential for us is produced in the light dependent reaction
O2
42The light dependent reaction converts light into chemical energy. identify which two energy carrying molecules are charged by the light dependent reaction for use in the light independent reaciton
ATP + NADPH
43idenify the esential organic molecule produced in the light independe reaction
glucose (C6H12O6)
44Other than sunlgiht, what other reactant of photosynthesis is used in the light dependent reaction
water (H2O)
45why is it important to have six CO2 entering the chloroplast
there are 6 carbons in glucose and only one carbon in each mlecule of CO2
46what is ATP?
energy storing molecule used in cells
47how is ATP produced
when energy from another sourc e(sunlight for exmaple) becomes available. energy an then be released or used to power a cellar process
48AtP molecules are reusable/nonreausable
reusable
49what is nadp
a mole that carries electrons, later to be given up so they can be oxidized
50nadp molecules are reusable/nonreausable
reusable
51how is atp and nadp important in the cycle between the light depend. and light independ. reactions?
they are charged up in the light depend. reactiosn, deliver energy to the lgiht independent reactions and cycle back to be recharged once again
52what does ATP stand for?
adenosine tri phosphate
53what does ADP stand for
Adenosine di-phosphate
54when ATP is used, a phosphate is removed. which three phosphate is removed?
outermost phosphate group is removed first (the phosphate not touching anything)
55when molecules gain electrons and/or hydrogen, they are considered...
reduced
56when molecules lose electrons and/or hydrogen, they are considered...
oxidized
57what does OIL RIG stand for?
Oxidation is Loss (Of electrons) and Reduction is Gain (of electrons)
58wht is the first step of light dependent reaction?
the chlorophyll molecules in photosystem II abosrbs light energy.
59which subatomic particles becomes super excited to the point where the chlorophyll atoms can no longer hold on to them?
electrons
60what is the first step of the light dependent reaction called?
photoactivation of electrons in photosystem II
61if photosystem II continues to aborb light, evetually it will run ot of electrons. which reactant of photosynthesis donates electrons to photosystem II (and is therefore oxidized)?
H20
62photolysis
The process of splitting water
63what two things are created in step two of light dependent reaction?
H+ ions and one oxygen atom (1/2 an O2 moleule)
64what is step three of the light depend. rxn
excited electrons pass from photosystem ii, to other proteins in the thylakoid membrane.
65what is the electron transport chain (ETC)
the series of elctron carrying proteins
66as electrons are passed along (in step three) some of their energy is used to actively transport which ion?
H+
67is the H+ ion in step three being pumped into or out the thylakoid
into
68how do we know step three is active transport?
H+ is moving aginst the gradient
69what is step three called of the light dependent rxn
electron transport chain and active transport
70what is the last carrier in teh ETC (electron transport chain) transfers the electrons to a 2nd photosystem
photosystem I
71what happens in step four of the light depend. rxn
the last carrier in the etc transfers the electrons to photosystem i. the electrons lost a lot of energy in the ETC however are once again photoactivated.
72suggest what pigment molecules photosystem i contains that captures sunlight / energizes eletros
chlrophyll (+ acessory pigments)
73photosystem i passes the electrons to another series of proteins --its called...
NADP+ reductase
74what kind of protein is NADP+ reductase
enzyme
75why is the name reductase in NADP+ reductase
it reduces (adds electrons to) NADP+ to create NADPH
76where does the created NADPH in step 4 of light dependent go?
calvin cycle
77what is step 5 of the process of light dependent called?
the proton gradient and atp formation
78what accumulates inside the thylakoids as a result of electron transport along the ETC?
H+
79this accumulation is a store of potential energy. through which molecule is the thylakoid membrane do H+ ions pass on their way back to the stroma/
ATP synthase
80what type of cellular transport occurs at step five?why?
facilitated diffusion: H+ moving down the concentration gradient (high to low); no ATP requited
81What molecule is produced or "charged up" as a result of the H+ diffusing through this molecule? (step five)
ATP
82what is phosphorulation
addition of phosphate
83what is the electrons used in step five?
to create the H+ gradient
84Where is there a high amount of H+ concentration
inside the thylakoid
85chemiosmosis
the movement of ions across a membrane, down their electrochemical graident
86where in the chloroplast do the light independent reactions take place?
stroma
87 what reactant of photosynthesis is used as the source of Carbon used to create glucose?
CO2
88What is the step one in the calvin cycle called?
Carbon fixation
89in the carbon fixation stage of the calvin cycle, what 5-carbon substrate does the enzyme rubisco attack CO2 molecules to?
RuBP
90RuBP + CO2 =
GP (glycerate 2-phosphate)
91wht is step 2 of light independ. rxn called
reduction of GP/Formation of precursor to glucose
92What molecule is being reduced in the Calvin Cycle?
GP
93What molecule is being oxidized in calvin cycle, how do u know?
NADPH, it is converted to NADP+
94other than reduced NADP, wat else is eneded to perform the reduction of GP
ATP
95what molecule is formed from the reduction of GP?
triose phosphate (TP)
96What is step three in the calvin cycle called
Regeneration of RuBP
97What happens during the regeneration of RuBP
for the calvin cycle to continue indefinitely, the starting substrate, RuBP needs to be regenerated. since TP has only three carbons and RuBP has five, several TP molecules (5 of them) are needed to create 3 RuBP (15 total carbons)
98what molecule is required to regenerate RuBP from the rearrangment of TP?
ATP
99since only 1/2 of glucose is made, how many times does the blow process need to occur?
twice (two times)
100how many total carbon dioxide molecules would be needed to make 1 complete molecule of glucose
six
101explain why increasing light level increases the rate of photsynthesis
more photoactivation, so more ATP + NADPH are produce. this means more photosynthesis can occur
102suggest why the rate of photosynthesis reaches a maximum after a certain light intensity is reached
at a certain point, we reach saturation of photosystems. at the saturation poin, all are in use + cannot work faster
103at what point in the graph is light intensity limiting photosynthesis? how do you know?
between light intesity of zero and where the rate of reaction plateaus. in this area of the graph, increaseing light intensity increases the rate of rxn.
104CO2 is a reactant in photosynthesis. what enzyme does it bind to?
rubisco
105suggest why increasing CO2 indefinitely canot increase the rate of photosynthesis glucose production indefintely
rubisco reaches a saturation point (all active sites are occupied by CO2)
106at what point of the graph is CO2 in exess? How do you know?
any point after the graph plateaus. after this point is reached, increasing CO2 does not increase the rate of rxn
107Why temp has a effect on photosynthesis
intial increases in temperature cause an increase in kinetic energy, meaning substrates are more likely to run into their enzymes + rate of rxn increases. eventually, temp. increases so much that enzymes denature + rate of rxn decreases.