1Growth of microorganisms in a culture media.
CULTURES
2Composed of a mixture of nutrients
CULTURE MEDIA
3culture media nutrients
CARBON, NITROGEN, SULFUR, PHOSPHORUS, HYDROGEN, OXYGEN, BUFFERS
4Types of culture media
Liquid, Semi-solid, Solid Medium
5TYPES OF CULTURE
Pure Culture, Mixed Culture, Stock Culture
6Composed of only one species
Pure Culture
7pure culture is used for what testing
Biochemical testing
8Composed of more than one species
Mixed Culture
9Test for Mixed culture
Subculture
10Composed of several culture species contained in a separate culture medium
Stock Culture
11How many species per culture medium
One
12Stock Culture is used for
Academic and industrial purposes
13Stock Culture is also knowned as
Reserved bacteria
14CLASSIFICATION OF CULTURE MEDIA
Consistency, Composition, Dispensing or Distribution Method, Use
15does not contain any amount of agar
LIQUID MEDIUM
16Allows growth of aerobes, anaerobes and facultative anaerobes.
LIQUID MEDIUM
17Example of liquid medium
Brain heart infusion, trypticase soy broth (TSB) and thioglycolate
18used to observe bacterial motility and detect indole and sulfide production.
SEMI-SOLID MEDIUM
19SEMI-SOLID MEDIUM contains how many agar
0.5% to 1%
20Example of Semi-solid medium
Sulfide Indole Motility (SIM) Medium
21contains 2% to 3% agar
SOLID MEDIUM
22Example of solid medium
Triple sugar Iron (TSI) agar, MacConkey (MAC) agar, Blood agar plate (BAP) and Chocolate agar plate (CAP)
23Medium in which all the components are known.
SYNTHETIC OR DEFINED MEDIUM
24Preferred for isolation of cyanobacterium and chemo organotrophs
SYNTHETIC OR DEFINED MEDIUM
25Example of synthetic or defined medium
BG-11 medium
26Medium in which some substances are unknown.
NON SYNTHETIC OR COMPLEX MEDIUM
27Components of non-synthetic or complex medium
Peptones, Meat and Yeast Extract
28Used for isolation of medically significant bacteria
NON-SYNTHETIC OR COMPLEX MEDIUM
29Example of NON-SYNTHETIC OR COMPLEX MEDIUM
Nutrient broth(NB) medium, TSB, MAC agar
30Used for obligate intracellular bacteria
TISSUE CULTURE MEDIUM
31Example of obligate intracellular bacteria
Rickettsia and Chlamydia
32Part of the body where you can find McCoy cells and W138 cells
Fibroblast
33Distributed into the dish or plate
PLATE MEDIA
34Prepared as either liquid, slant, butt and slant or butt
TUBE MEDIA
35Example of TUBE MEDIA
Triple sugar iron (TSI), SIM, Simmon's citrate agar, Lysine iron agar (LIA)
36Routinely used in laboratory and without additional supplement
SIMPLE MEDIA, GENERAL PURPOSE MEDIA AND SUPPORTIVE MEDIA
37Support the growth of most non-fastidious bacteria
SIMPLE MEDIA, GENERAL PURPOSE MEDIA AND SUPPORTIVE MEDIA
38Used to propagate the growth of certain group of bacteria from a mixture of organisms
ENRICHMENT MEDIA (LIQUID TYPE MEDIA)
39Example of enrichment media (liquid type media)
Alkaline peptone water, Selenite F, Thioglycolate, Tetrathionate, Gram-negative (GN) broth and Lim broth
40Alkaline peptone water is used to detect what
Vibrio
41Selenite F is used to detect what
Salmonella
42Lim broth is used to detect what
Group b streptococcus
43Media with additional supplement such as BLOOD, VITAMINS and YEAST EXTRACT
ENRICHED MEDIA and NON-SELECTIVE MEDIA
44Blood used for BAP
Sheep's blood, Horse's blood, O+
45Differentiate hemolytic pattern of streptococci
Blood agar plate (BAP)
46recovery of Haemophilus
Chocolate agar plate (CAP)
47These media allow the visualization of metabolic differences between group of bacteria
DIFFERENTIAL MEDIA
48Example of DIFFERENTIAL MEDIA
MAC, BAP, Eosin methylene blue (EMB) and Hektoen
50media incorporated with ANTIBIOTICS, DYES, or CHEMICALS
SELECTIVE MEDIA
51Example of SELECTIVE MEDIA
MAC, Bismuth sulfate agar (BSA), Mannitol Salt Agar (MSA) and Thayer-Martin Agar (TMA)
52Crystal/Gentian violet, basic/carbol fuchsin and bile salt
Gram Positive Bacteria
53Potassium tellurite and Sodium azide
Gram Negative Bacteria
54Alcohol and chloralhydrate
Swarming Bacteria
55Positive for Swarming
P vulgaris
56Negative for Swarming
P mirabilis
57Bile salt and dyes; pH indicator BROMTHYMOL BLUE
Hektoen Enteric Agar (HEA)
58bile salts and crystal violet
MacConkey (MAC) Agar
59Differentiate SHIGELLA and SALMONELLA
Xylose Lysine Desoxycholate (XLD) Agar
60Support growth of STAPHYLOCOCCUS AUREUS
Mannitol Salt Agar (MSA)
61Selective for NEISSERIA sp.
Thayer-Martin Agar (TMA)
62Used to isolate bacteria with SPECIFIC GROWTH REQUIREMENTS.
SPECIAL MEDIA
63Example of SPECIAL MEDIA
Lowenstein-Jensen Medium and Thiosulfate citrate-bile salts-sucrose (TCBS) agar
64Lowenstein-Jensen Medium is used for
Mycobacterium tuberculosis
65Thiosulfate citrate-bile salts-sucrose (TCBS) agar is used to detect
Vibrio cholerae