CULTURE MEDIA
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Growth of microorganisms in a culture media.
CULTURES
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Composed of a mixture of nutrients
CULTURE MEDIA
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culture media nutrients
CARBON, NITROGEN, SULFUR, PHOSPHORUS, HYDROGEN, OXYGEN, BUFFERS
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Types of culture media
Liquid, Semi-solid, Solid Medium
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TYPES OF CULTURE
Pure Culture, Mixed Culture, Stock Culture
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Composed of only one species
Pure Culture
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pure culture is used for what testing
Biochemical testing
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Composed of more than one species
Mixed Culture
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Test for Mixed culture
Subculture
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Composed of several culture species contained in a separate culture medium
Stock Culture
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How many species per culture medium
One
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Stock Culture is used for
Academic and industrial purposes
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Stock Culture is also knowned as
Reserved bacteria
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CLASSIFICATION OF CULTURE MEDIA
Consistency, Composition, Dispensing or Distribution Method, Use
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does not contain any amount of agar
LIQUID MEDIUM
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Allows growth of aerobes, anaerobes and facultative anaerobes.
LIQUID MEDIUM
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Example of liquid medium
Brain heart infusion, trypticase soy broth (TSB) and thioglycolate
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used to observe bacterial motility and detect indole and sulfide production.
SEMI-SOLID MEDIUM
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SEMI-SOLID MEDIUM contains how many agar
0.5% to 1%
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Example of Semi-solid medium
Sulfide Indole Motility (SIM) Medium
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contains 2% to 3% agar
SOLID MEDIUM
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Example of solid medium
Triple sugar Iron (TSI) agar, MacConkey (MAC) agar, Blood agar plate (BAP) and Chocolate agar plate (CAP)
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Medium in which all the components are known.
SYNTHETIC OR DEFINED MEDIUM
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Preferred for isolation of cyanobacterium and chemo organotrophs
SYNTHETIC OR DEFINED MEDIUM
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Example of synthetic or defined medium
BG-11 medium
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Medium in which some substances are unknown.
NON SYNTHETIC OR COMPLEX MEDIUM
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Components of non-synthetic or complex medium
Peptones, Meat and Yeast Extract
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Used for isolation of medically significant bacteria
NON-SYNTHETIC OR COMPLEX MEDIUM
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Example of NON-SYNTHETIC OR COMPLEX MEDIUM
Nutrient broth(NB) medium, TSB, MAC agar
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Used for obligate intracellular bacteria
TISSUE CULTURE MEDIUM
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Example of obligate intracellular bacteria
Rickettsia and Chlamydia
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Part of the body where you can find McCoy cells and W138 cells
Fibroblast
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Distributed into the dish or plate
PLATE MEDIA
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Prepared as either liquid, slant, butt and slant or butt
TUBE MEDIA
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Example of TUBE MEDIA
Triple sugar iron (TSI), SIM, Simmon's citrate agar, Lysine iron agar (LIA)
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Routinely used in laboratory and without additional supplement
SIMPLE MEDIA, GENERAL PURPOSE MEDIA AND SUPPORTIVE MEDIA
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Support the growth of most non-fastidious bacteria
SIMPLE MEDIA, GENERAL PURPOSE MEDIA AND SUPPORTIVE MEDIA
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Used to propagate the growth of certain group of bacteria from a mixture of organisms
ENRICHMENT MEDIA (LIQUID TYPE MEDIA)
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Example of enrichment media (liquid type media)
Alkaline peptone water, Selenite F, Thioglycolate, Tetrathionate, Gram-negative (GN) broth and Lim broth
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Alkaline peptone water is used to detect what
Vibrio
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Selenite F is used to detect what
Salmonella
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Lim broth is used to detect what
Group b streptococcus
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Media with additional supplement such as BLOOD, VITAMINS and YEAST EXTRACT
ENRICHED MEDIA and NON-SELECTIVE MEDIA
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Blood used for BAP
Sheep's blood, Horse's blood, O+
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Differentiate hemolytic pattern of streptococci
Blood agar plate (BAP)
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recovery of Haemophilus
Chocolate agar plate (CAP)
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These media allow the visualization of metabolic differences between group of bacteria
DIFFERENTIAL MEDIA
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Example of DIFFERENTIAL MEDIA
MAC, BAP, Eosin methylene blue (EMB) and Hektoen
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Lactose fermenter
MAC
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media incorporated with ANTIBIOTICS, DYES, or CHEMICALS
SELECTIVE MEDIA
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Example of SELECTIVE MEDIA
MAC, Bismuth sulfate agar (BSA), Mannitol Salt Agar (MSA) and Thayer-Martin Agar (TMA)
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Crystal/Gentian violet, basic/carbol fuchsin and bile salt
Gram Positive Bacteria
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Potassium tellurite and Sodium azide
Gram Negative Bacteria
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Alcohol and chloralhydrate
Swarming Bacteria
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Positive for Swarming
P vulgaris
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Negative for Swarming
P mirabilis
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Bile salt and dyes; pH indicator BROMTHYMOL BLUE
Hektoen Enteric Agar (HEA)
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bile salts and crystal violet
MacConkey (MAC) Agar
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Differentiate SHIGELLA and SALMONELLA
Xylose Lysine Desoxycholate (XLD) Agar
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Support growth of STAPHYLOCOCCUS AUREUS
Mannitol Salt Agar (MSA)
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Selective for NEISSERIA sp.
Thayer-Martin Agar (TMA)
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Used to isolate bacteria with SPECIFIC GROWTH REQUIREMENTS.
SPECIAL MEDIA
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Example of SPECIAL MEDIA
Lowenstein-Jensen Medium and Thiosulfate citrate-bile salts-sucrose (TCBS) agar
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Lowenstein-Jensen Medium is used for
Mycobacterium tuberculosis
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Thiosulfate citrate-bile salts-sucrose (TCBS) agar is used to detect
Vibrio cholerae
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