1CAR-T stands for
Chimeric antigen Receptor - t cell
2what's "chimeric" in a chimeric antigen receptor t-cell ?
the antigen receptor contains elements from both antibodies and t cell receptors
3What does "chimeric" mean
made of many different parts
4what are "antigen receptors"
protein receptors on a surface of a cell that targets a specific substance
5what is the significance of chimeric antigen receptors on a T cell
Allows the patient's T-cell to target a specific antigen found on patient's cancer
6What does LVV stand for
Lentiviral vector
7PBMC stands for
peripheral blood mononuclear cell
8what does peripheral blood mononuclear cell mean
blood cells with a round nucleus
9What do PBMC's consist of?
Platelets
10Examples of Lymphocytes?
T cells
11Examples of Myeloid cells?
Dendritic cells
12Ex-vivo means
occuring outside the human body
13autologous means:
To be of patient's own cells
14BCMA stands for
B cell maturation antigen
15what kind of cancer is multiple myeloma
cancer of the plasma cells
16what happens when someone has cancer of the plasma cells
body makes too many plasma cells and too many antibodies
17What do B cells do
develop into plasma cells when antigens are present
18what is leukapheresis
the gathering of patient cells from blood
19Where does leukapheresis take place
in a clinic
20How long does apheresis take?
4 hours
21When does BMS receive the patient's bag of cells?
After apheresis
22What is the general process of Ide-cel manufacturing?
Bulk Media Prep
23What is Day -1
PBMC Isolation & PBMC Cryopreservation
24What is Day 0
PBMC Thaw & Culture initiation
25What is Day 1
LVV Transduction
26What is Day 2
48 hour incubation
27What is Day 3-9
Static Culture Maintenance and Expansion
28What is Day 10
Drug Product Harvest
29Is ide-cel an aseptic process or sterile process?
Aseptic
30Drug Product Harvest is which day
Day 10
31Drug Harvest Cryopreservation is which day
Day 10
32Static Culture Maintenance and Expansion is which day
Day 3-9
3348 hour incubation is which day
Day 2
34LVV Transduction is which day
Day 1
35PBMC Thaw & Culture initiation is which day
Day 0
36PBMC isolation is which day
Day -1
37PBMC Cryopreservation is which day
Day -1