Ide-cel - Bulk Media Preparation

11 flashcardsby krishan
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1
what is the growth medium for T cells
T cell Growth Media TCGM
2
How many components are there to TCGM
5 components
3
What are the five components to TCGM
1 XVIVO-15
2 HEPES
3 GlutaMAX
4 HABS
5 Recombinant human IL2
4
What is XVIVO-15
Serum free media
5
What is HEPES
Is a buffering agent that maintains physiological pH in cell culture
6
HEPES stands for
short for HydroxyEthyl Piperazine EthaneSulfonic Acids
7
What is GlutaMAX used for
Minimizes toxic ammonia build up to improve cell health
8
What does HABS stand for
Heat Inactivated Human AB Serum
9
What is HABS used for
to simulate the natural environment a T cell normally exists in
10
What is recombinant human IL2 used for
cell growth factor that is required for T cells to grow and proliferate
11
A single run of Bulk Media prepares enough TCGM for how many lots?
Three patient lots
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