1What do all plants contain?
Different photosynthetic pigments
2What do pigments in leaves absorb?
Light of different wavelengths
3What does having different pigments allow?
Absorption of more light wavelengths
4What technique separates pigments in leaves?
Chromatography
5What is used in paper chromatography?
Paper as stationary phase
6What is used in thin-layer chromatography?
Silica gel as stationary phase
7What does the solvent carry up the plate?
Dissolved pigments
8What separates pigments during chromatography?
Relative solubility in the solvent
9What does the Rf value represent?
Distance pigment travels/distance solvent front
10How is Rf value calculated?
Distance traveled by pigment/distance by solvent
11What does TLC compare?
Pigments in different plants
12What kind of plants are compared using TLC?
Shade-tolerant and shade-intolerant
13What should be worn during TLC?
Lab coat, eye protection
14What solvent is used in TLC?
Anhydrous sodium sulfate with propanone
15What is ground in the solution?
Plant leaves
16What collects after centrifuging ground leaves?
Supernatant
17How are pigments separated on the plate?
By differences in solubility
18What is NADP's role in photosynthesis?
Electron acceptor
19What happens when DCPIP is reduced?
It loses its blue color
20What indicates the rate of dehydrogenase?
Rate of color change of DCPIP
21What equipment is used to measure absorbance?
Colorimeter
22What is the maximum wavelength of light used?
605 nm
23What buffer is used in dehydrogenase experiments?
pH 7 isolation buffer
24What does centrifuging isolated chloroplasts create?
A pellet
25What solution is added to the pellet?
Buffer and DCPIP
26What happens as dehydrogenase reduces DCPIP?
Absorbance decreases
27How is the absorbance recorded?
Every 2 minutes for 10 minutes
28What is the role of a bench lamp?
Light source for chloroplast activity
29What does the rate of absorbance decrease indicate?
Dehydrogenase activity
30What does practice improve for experiments?
Results reliability
31What must animals not be during experiments?
Harmed