photosynthesis experiments

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1
What do all plants contain?
Different photosynthetic pigments
2
What do pigments in leaves absorb?
Light of different wavelengths
3
What does having different pigments allow?
Absorption of more light wavelengths
4
What technique separates pigments in leaves?
Chromatography
5
What is used in paper chromatography?
Paper as stationary phase
6
What is used in thin-layer chromatography?
Silica gel as stationary phase
7
What does the solvent carry up the plate?
Dissolved pigments
8
What separates pigments during chromatography?
Relative solubility in the solvent
9
What does the Rf value represent?
Distance pigment travels/distance solvent front
10
How is Rf value calculated?
Distance traveled by pigment/distance by solvent
11
What does TLC compare?
Pigments in different plants
12
What kind of plants are compared using TLC?
Shade-tolerant and shade-intolerant
13
What should be worn during TLC?
Lab coat, eye protection
14
What solvent is used in TLC?
Anhydrous sodium sulfate with propanone
15
What is ground in the solution?
Plant leaves
16
What collects after centrifuging ground leaves?
Supernatant
17
How are pigments separated on the plate?
By differences in solubility
18
What is NADP's role in photosynthesis?
Electron acceptor
19
What happens when DCPIP is reduced?
It loses its blue color
20
What indicates the rate of dehydrogenase?
Rate of color change of DCPIP
21
What equipment is used to measure absorbance?
Colorimeter
22
What is the maximum wavelength of light used?
605 nm
23
What buffer is used in dehydrogenase experiments?
pH 7 isolation buffer
24
What does centrifuging isolated chloroplasts create?
A pellet
25
What solution is added to the pellet?
Buffer and DCPIP
26
What happens as dehydrogenase reduces DCPIP?
Absorbance decreases
27
How is the absorbance recorded?
Every 2 minutes for 10 minutes
28
What is the role of a bench lamp?
Light source for chloroplast activity
29
What does the rate of absorbance decrease indicate?
Dehydrogenase activity
30
What does practice improve for experiments?
Results reliability
31
What must animals not be during experiments?
Harmed
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