bchm338

Protein targeting lignad, linker, read our or pull down

target binding site of receptors and block signal
target active site and impact enzyme finction
allosterically bind and inhibit active or binding site function

safe
selective enough to limit side effects
may have undefined mehcanism

needs to address specific biological question
needs to be selective
defined mechanism
access cannot be restricted

controlled release
target specificity
target amplification
delayed drug release

extended life
stability
selectivity
easier to synthesis

easily synthesised
widely emplyed
can impact interactions

compound that alters property of system of interest then identify the modulated dene product which is respnsible.

compounds are exposed to more biologically relevant contexts

dont know the target
target discovery may be dificult
lot of subsequent effort needed to discover moelcular targets and understand their selectivity and mechanism

start with known target seek to investigate its function using perturbation experriments.

identifies target protein and characterises the protein-compound interaction
expose test system to compound, analyses phenotype and compart controls