PMB 150 plant cell biology part 2

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1
In response to light, where does auxin accumulate?
shaded side
2
how is auxin gradient established?
PIN proteins polar auxin transport
3
how does auxin induce gene expression?
auxin attaches to the SCF complex and AUX/IAA, which degrades AUX/IAA and allows ARF to transcribe
4
how were GAs originally identified?
fungal compounds that promote stem elongation
5
what 4 things do GAs promote?
stem elongation, seed germination, flowering, and fruit development
6
how does GA promote growth?
increases cell expansion and division
7
how do GAs promote elongation?
cell wall loosening and stabilizing the orientation of cortical microtubules
8
loss of function mutants in GA synthesis are
severely dwarfed
9
mutants in GA3ox genes have reduced _________ but not ___________
height, seed production
10
how does GA induce gene expression?
GA attaches to the SCF complex and DELLA proteins, which degrades DELLA and allows PIF to transcribe
11
biosynthesis mutants (can / cannot) be rescued by GA application
can
12
GID1 encodes ______, and mutants (can / cannot) be rescued by GA application
a GA receptor, cannot
13
at high salt levels _______ is necessary for survival
DELLA activity
14
ABA promotes
desiccation tolerance and dormancy through stabilizing DELLA proteins
15
seed germination requires ___________ and ______________
elimination of ABA, production of GA
16
GA signalling transcribes _________, which ___________
amylase, breaks down the starches stored in the seed into simple sugars
17
T-DNA includes biosynthesis genes for ______ and ________, which produces a _______
auxin, CK, tumor
18
if T-DNA only has auxin genes, the tumor produced will be
rooty
19
if T-DNA only has CK genes, the tumor produced will be
shooty
20
elevated CK production promotes __________ and restricts _______
shoot growth, root growth
21
IPT genes synthesizes
CK
22
In CK signalling, CK first binds to __________, which ________. Finally, ___________
membrane-bound receptor HKs, transfers phospho groups to HPTs, HPTs transfer the phosphoryl group to response regulators
23
What are the 3 types of CK regulators, and are they positive or negative?
type A and C are negative regulators, type B is positive
24
type C ARRs ____________
remove phosphoryl groups from the system via phosphatase activity
25
type A ARRs negatively regulate CK signalling by
competing for phospho groups with type B
26
type B ARRs have a ________ domain, which allows it to ________
DNA binding, transcribe
27
CK can directly stimulate ________, but auxins repress __________ by _______
lateral bud outgrowth, outgrowth, downregulating cytokinin synthesis at the node
28
CK delays
leaf senescence
29
Loss-of-function mutants affecting CK synthesis or signalling are
drought tolerant
30
BR biosyntehsis involves a
metabolic grid
31
grafting studies suggest that BR
is not transported long distances
32
BR is perceived at the ________ by ___________
cell membrane, receptor and co-receptor
33
BRI1 and BAK1 interact through
double lock mechanism
34
the 4 cytoplasmic BR signalling events are:
Phosphorylation, De-phosphorylation, Protein degradation, Transcriptional control
35
BIN2 is
a protein kinase that in absence of BR phosphorylates and targets TF for degradation
36
BIN2 is inactivated by
BR signalling
37
BR promotes
cell division and cell elongation
38
BRI1 mutant in barley has
dwarfed phenotype and higher yields
39
auxin induces expression of a _____ synthesis enzyme
BR
40
what 2 major hormones regulate plant response to stress?
ETH and ABA
41
ETH regulates these 4 things
fruit ripening, organ expansion, senescence, stress responses
42
When germinating in the dark, impeded seedlings produce
ETH
43
The response to ethylene is very (slow / fast)
fast
44
The two key ethylene biosynthesis enzymes are
ACS and ACO
45
Soil bacteria can reduce ethylene levels in plants by
deactivating ACC
46
ETR1 encodes
ETH receptor
47
Ethylene receptors ___________ regulate responses
negatively
48
what is the order of action of the genes in ethylene signaling pathway?
ETH --> ETR1 --> CTR1
49
if CTR1 is active, ETH responses are ____
off
50
in the abscence of ETH, CTR1
phosphorylates EIN2
51
ETH restricts _______ in the dark
elongation of the shoot and root
52
ETH's effects are mediated by ______ in the root
auxin
53
ETH promotes _______ in flowers
petal senescence
54
Ripening includes these 4 hallmarks of scenescence
Changes in cell wall structure, Pigment accumulation, Flavor and aromatic volatile production, Conversions of starches to sugars
55
hypoxia means
lack of oxygen
56
hypoxia by flooding increases _________ synthesis
ETH
57
in response to hypoxia, ETH induces
cell death or cell separation and formation of air channels for oxygen to move into roots
58
In deepwater rice, ethylene induces
internode elongation
59
ETH elongation response is encoded by the TFs _____ and __________
SNORKEL1, SNORKEL2
60
ABA is synthesized in
the plastid and cytoplasm
61
ABA is derived from
a carotenoid plant pigment
62
After water stress ABA accumulates in
the veins and guard cells
63
ABA biosynthesis mutants
lose water rapidly
64
Plants initially sense drought through their __________ and mediate the response through ___________
roots, tropism and signaling to shoots
65
________ receptors are required for ABA responses
PYR
66
Receptors bind ABA in
a complex with phosphatases
67
phosphorylation of _______ promotes ABA response
ion channels and transcription factors
68
ABI1 encodes
PP2C protein phosphatase
69
ABI1 mutants are all
ABA insensitive
70
________ can be used to monitor guard cell responses
thermal imaging
71
Mutants impaired in ABA synthesis or signaling show reduced _______ in response to a decrease in humidity, so the mutants are (hotter / cooler) than WT
stomatal closure, cooler
72
ABA signalling involves ABA binding to ________, which attaches to __________, which activates ________, which phosphorylates ________
PYR receptors, protein phosphatases, protein kinases, transcription factors
73
ABA promotes (turgid / flaccid) guard cells in drought
flaccid
74
_______ controls guard cell turgor
Osmotic movement of water
75
water stress and ABA promote _______ at the expense of _________
root growth, shoot growth
76
Drought stress suppresses ______ root growth while ________ root elongation is maintained
lateral, primary
77
In many fruits, a peak of ABA synthesis occurs
during ripening or the onset of ripening
78
ABA plays a more dominant role than ETH in ________ fruits
non-climacteric
79
A lower ABA concentration leads to ______ fruit ripening
delayed
80
In young leaves ABA accumulation does not
inhibit immunity
81
In old leaves ABA accumulation interferes with
immune responses
82
Biotic stress and development involves these 2 hormones
SA and JA
83
SA defends from _______ pathogens
biotrophic
84
The major SA synthesis pathway is through _______ and takes place in the _______
ICS, chloroplast
85
the minor SA synthesis is through _______ which takes place in the _______
PAL, cytoplasm
86
SA is ________ in the acidic cell wall and _________ in the cytoplasm
uncharged, negatively charged
87
there is evidence for _______ transport of SA
carrier-mediated
88
NPR1 is a major activator of______
SA responses
89
SA accumulation contributes to a ________ environment, which causes NPR1 to ________ and move into ________ to ________
reducing, monomerize, the nucleus, activate gene transcription
90
In the absence of SA and activated NPR1, defense genes are
repressed by negative regulators
91
NPR1 binds ______ transcription factors and promotes ______
TGA and WRKY, DNA binding
92
TGA2 is both a ______ of SA transcription
repressor and activator
93
WRKYs are a large family of _____ involved in _____ signalling
TFs, defense
94
TGAs and WRKYs are negative regulators, until _______, when they become
NPR1 binds to them, positive regulators
95
pathogen recognition causes ______ accumulation, which leads to the production of SA
ROS
96
grafting studies suggest that SA (is / isn't) the mobile signal for SAR
isn't
97
constant SA production has high immunity but
stunted growth
98
Growth vs defense balance is mediated through
DELLA proteins
99
what are 2 Modes of Bypassing the Growth–Immunity Tradeoff?
35S promoter (constant overexpression), TBF1 promoter (only in presence of pathogen)
100
SA and JA _______ each other
antagonize
101
JAs respond to ______ pathogens
necrotrophic
102
JA can be conjugated to amino acids by
JAR1
103
for JA, _____ isthe most active compound whereas _____ may be a transport form
JA-Ile, MeJA
104
CO1 encodes ______ and is similar to _____ in the auxin signalling pathway
a JA receptor, TIR1
105
COI1 is a component of the
ubiquitin ligase complex
106
JA binds to the SCF complex and connects it to _______, which leads to that protein's degradation and allows _______ to transcribe
JAZ, Myc
107
what does TPC do?
represses transcription in absence of hormones
108
What does NINJA do in JA signalling?
without JA, NINJA binds to JAZ, indirectly connecting it to TPC
109
JA accumulates (slow / fast) after wounding
fast
110
Plants produce _______that interfere with digestion
proteinase inhibitors
111
Coronatine is a mimic of _________. it stimulates _______ responses and represses ______
responses
JA-Ile, JA, SA
112
in addition to pathogen defense, JA also controls
cell cycle, root extension, leaf senescence, stomata closure, and mutualistic interactions
113
SLs promote _______ and _________, but inhibits __________
germination of parasitic Striga plants, associations with AM fungi, shoot branching
114
symbiotic association with AM fungi helps plants
assimilate nutrients from the soil
115
In mutant plants unable to make SLs,
more shoot branches grow
116
Karrikins
A butanolide compound from smoke that promotes seed germination
117
Karrikins are perceived by
KAI2 receptors
118
SL receptors for shoot branching evolved from
duplicated KAI2 receptors
119
SL signaling is homologous to the auxin pathway, in which ______ is like AUX/IAA, and ______ is like ARF
D53, SPL
120
D14 is
a soluble receptor that binds SLs
121
in shoots, SLs decrease
polar auxin transport
122
Phosphate-starved plants suppress _______ and enhance _________
shoot growth, root growth
123
in nutrient-poor soil, SL synthesis _____
increases
124
name 3 processes ethylene controls in the plant
fruit ripening, senescence, stress response
125
What is the ethylene triple response?
inhibition of root and hypocotyl elongation, exaggerated tightening of the apical hook, and swelling of the hypocotyl
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