1what component of the diluting fluid stains eosinophils red
phloxine
2what component of the diluting fluid lyses rbcs
propylene glycol
3what component of the diluting fluid inhibits wbc clumping
heparin
4what component of the diluting fluid lyses all wbcs except eosinophils
sodium carbonate
5what are the components of phloxine diluting fluid
phloxine
propylene glycol
sodium carbonate
distilled water
6what are the components of pilot's solution
phloxine
propylene glycol
sodium carbonate
heparin
distilled water
7what are the components of manner's diluting fluid
phloxine
urea
trisodium citrate
distilled water
8what are the components of Randolph's diluting fluid
phloxine
calcium chloride
propylene glycol
9up to what mark is anticoagulated whole blood aspirated
1 mark
10up to what mark is the diluting fluid aspirated
11 mark
11how long is the pipette mixed when using pipette shaker
10 minutes
12how long is the pipette mixed if manual
30-45 seconds
13how long are the cells allowed to settle before counting
3 minutes
14what is the formula for counting eosinophils
(eosinophils counted ÷ 9) 100
15what is the unit for solving eosinophils counted
eosinophils/mm³
16what is the reference range for eosinophils
150-300 eosinophils/mm³
17condition resulting to decreased eosinophils
eosinopenia
18condition resulting to increased eosinophils
eosinophilia
19what are the two conditions that result in eosinopenia
hyperadrenalism
cushing's disease
20when a person has hyperadrenalism, what hormone is produced that inhibits release of eosinophils from the BM
corticosteroids
21situations that cause eosinophilia
allergic reaction
parasitic invasion
certain leukemias
22what is the test used for normal adrenal function
Thorn's test
23in Thorn's test, what is taken after the fasting of the patient
baseline eosinophil count
24T or F:
in Thorn's test, the patient is not required to fast
25what is given to the patient through injection after taking the baseline eosinophil count in Thorn's test
adrenocorticosteroid hormone
26after injecting patient with ACTH, how many hours until another eosinophil count is determined
4 hours
27in a normal adrenal condition, the second eosinophil count is ____ than the fasting count
28hypoadrenalism
- Addison's disease
- Cushing's disease
29hyperadrenalism
- Cushing's disease
- Addison's disease
30in Addison's disease, eosinophil count ____ from the second absolute count
31in Cushing's disease, eosinophil count ____ from the second absolute count
32what method is used in absolute basophil count and what is it also known as
toluidine blue method
(aka: cooper and cruickshank method)
33what component of toluidine blue method lyses rbcs and render basophil granules insoluble
cetylpyridinium chloride
34what component of toluidine blue method is used as a mordant to improve the staining qualities of toluidine blue
ammonium sulfate
35what component of toluidine blue method is used to prevent platelet coagulation
EDTA
36basophils are seen as (appearance)
purple-red
metachromatically stained
37reference range for absolute basophil count
0-2×10⁹/L