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1
what component of the diluting fluid stains eosinophils red
phloxine
2
what component of the diluting fluid lyses rbcs
propylene glycol
3
what component of the diluting fluid inhibits wbc clumping
heparin
4
what component of the diluting fluid lyses all wbcs except eosinophils
sodium carbonate
5
what are the components of phloxine diluting fluid
phloxine
propylene glycol
sodium carbonate
distilled water
propylene glycol
sodium carbonate
distilled water
6
what are the components of pilot's solution
phloxine
propylene glycol
sodium carbonate
heparin
distilled water
propylene glycol
sodium carbonate
heparin
distilled water
7
what are the components of manner's diluting fluid
phloxine
urea
trisodium citrate
distilled water
urea
trisodium citrate
distilled water
8
what are the components of Randolph's diluting fluid
phloxine
calcium chloride
propylene glycol
calcium chloride
propylene glycol
9
up to what mark is anticoagulated whole blood aspirated
1 mark
10
up to what mark is the diluting fluid aspirated
11 mark
11
how long is the pipette mixed when using pipette shaker
10 minutes
12
how long is the pipette mixed if manual
30-45 seconds
13
how long are the cells allowed to settle before counting
3 minutes
14
what is the formula for counting eosinophils
(eosinophils counted ÷ 9) 100
15
what is the unit for solving eosinophils counted
eosinophils/mm³
16
what is the reference range for eosinophils
150-300 eosinophils/mm³
17
condition resulting to decreased eosinophils
eosinopenia
18
condition resulting to increased eosinophils
eosinophilia
19
what are the two conditions that result in eosinopenia
hyperadrenalism
cushing's disease
cushing's disease
20
when a person has hyperadrenalism, what hormone is produced that inhibits release of eosinophils from the BM
corticosteroids
21
situations that cause eosinophilia
allergic reaction
parasitic invasion
certain leukemias
parasitic invasion
certain leukemias
22
what is the test used for normal adrenal function
Thorn's test
23
in Thorn's test, what is taken after the fasting of the patient
baseline eosinophil count
24
T or F:
in Thorn's test, the patient is not required to fast
in Thorn's test, the patient is not required to fast
- T
- F
25
what is given to the patient through injection after taking the baseline eosinophil count in Thorn's test
adrenocorticosteroid hormone
26
after injecting patient with ACTH, how many hours until another eosinophil count is determined
4 hours
27
in a normal adrenal condition, the second eosinophil count is ____ than the fasting count
- 50% higher
- 50% lower
28
hypoadrenalism
- Addison's disease
- Cushing's disease
29
hyperadrenalism
- Cushing's disease
- Addison's disease
30
in Addison's disease, eosinophil count ____ from the second absolute count
- increase
- decrease
31
in Cushing's disease, eosinophil count ____ from the second absolute count
- increase
- decrease
32
what method is used in absolute basophil count and what is it also known as
toluidine blue method
(aka: cooper and cruickshank method)
(aka: cooper and cruickshank method)
33
what component of toluidine blue method lyses rbcs and render basophil granules insoluble
cetylpyridinium chloride
34
what component of toluidine blue method is used as a mordant to improve the staining qualities of toluidine blue
ammonium sulfate
35
what component of toluidine blue method is used to prevent platelet coagulation
EDTA
36
basophils are seen as (appearance)
purple-red
metachromatically stained
metachromatically stained
37
reference range for absolute basophil count
0-2×10⁹/L
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