1Describe the TOM complex:
- Required for all transport across the outer membrane.
- Transports proteins into the matrix and inner membrane.
- TOM complex has a receptor binding region.
2TIM23
- Transports proteins into the matrix and inner membrane.
- Electrochemical gradient assists TIM 23 in the transport of proteins.
- Tim 23 will recognize stop transfer sequences and embed them into the inner membrane.
3What is responsible for cutting off the signal sequence to keep proteins in their destinations?
A peptidase
4When is OXA complex used?
- When proteins come from TIM22/23 or mitochondrial ribosome synthesis
- OXA mediates the insertion of proteins synthesized inside mitochondria into the inner membrane.
5TIM22
- Mediates the insertion of subclass inner membrane proteins.
- Carrier proteins that transport ADP, ATP, and phosphate groups.
- Proteins with localization signals, not N terminus signals.
- ONLY imports carrier proteins.
6SAM
Inserts protein into outermembrane in the form of Beta barrels.
7Mito. hsp70
Will pull in ATP-bound proteins through TIM23, this hydrolyzes ATP to ADP then causes the protein to fold up. Mito.hsp60 ensures protein is folded up through TIM23.
8Describe MIA 40
- Helps proteins through TOM
- Is partial to cysteine rich proteins
- Good at oxidation of sulfhydryl groups (disulfide bridge)