mod7 culture transfer techniques

another word for transfering microbes from one media type to another

in microbiology laboratory, media types of AGAR SLANTS, AGAR BUTTS, AND AGAR BROTHS are used.

a solid media in a test tube with a slated surface on which to culture the microorganism. Typically inoculated by streaking the surface of the slant with a sterile loop.

solid media which does not have a slanted surface. Typically inoculated by stabbing the media with a sterile needle.

solid media which is contained in a Petri plate, providing optimal surface on which to culture organisms.
Inoculated by streaking the surface with sterile loop.

a liquid medium which can be inoculated by a sterile loop, needle, or pipette.

STREAK PLATE METHOD

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false


sterilize the loop or needle by holding the wire in the flame of alcohol lamp until its yellow hot.While holding the sterile loop or need, ise the little finger to remove the cap or cotton plug of. the culture media.

STREAK PLATE METHOD

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false

Briefly heat the mouth of the tube in the flame before inserting the loop or needle for an inoculum. Fish a loopful of specimen, heat the mouth of the tube and replace the cap or cotton plug.

STREAK PLATE METHOD

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false

raise the cover of the culture plate then insert loop or need and streak in a counter-clockwise direction or singles until the entire surface has been streak. Then remove loop a sterilize with lysol and running water

STREAK PLATE METHOD

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false

invert the plate and incubate for 1 week. Incubate at 27 degrees celcius.

TUBE BROTH MEDIA

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false

steps 1-4 are the same with streaking plate method. Do not flame the loop.

TUBE BROTH MEDIA

A-1st stment true,2nd stment false
B-1st stment false, 2nd stment true
C-both stment is true
D-both stment is false

pull out the loop and flame the mouth of the tube. then make sure not to cover the tube.