Microbio M6

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1
it accomplished by growing or culturing them on the surface of solid nutrient media
Media Isolation
2
what is the content of Normal mixture of Medium
Protein digest (peptone and tryptone)
Inorganic salt
1.5% agar
3
True or False

The standard or general purpose of media is to support the growth of wide variety of bacteria?
True
4
It contains high concentration of Sodium Chloride
Mannitol Salt Agar
5
A. The 1st Statement is correct. The 2nd Statement is incorrect.
B. The 1st Statement is incorrect. The 2nd Statement is correct.
C. Both Statements are correct.
D. Both Statements are incorrect.

Mannitol salt agar contains a high concentration of sodium chloride. It inhibits the growth of most organisms but does not permit staphylococci to grow
A. The 1st statement is correct. The 2nd statement is incorrect.
6
Which is true about Differential Media?
A. It compound to allow group for microorganism to be visually distinguish by the appearance of the colony or the surrounding media
B. Usually on the basis of some biochemical difference between the one group
C. Used to distinguish between bacterial cultures based on their biochemical structure
D. AOTA
A. It compound to allow group for microorganism to be visually distinguish by the appearance of the colony or the surrounding media
7
Food source of blood agar
Blood
8
Selective or Differential

Blood Agar
Selective
9
It allows bacteria to be distinguished by the type of hemolysis produced
Blood Agar
10
It is selective for gram-negative coliforms. This media can differentiate lactose fermenting and non-lactose fermenting bacteria
Eosin-methylene blue agar
11
It differentiates between two major coliforms which are E. Coli and Enterobacter aerogenes
Eosin-methylene blue agar or EMB
12
Selective or Differential

MHA
Selective
13
A small, green-metallic sheen
E. Coli
14
A large, rose color can be found inside the stomach
Enterobacter aerogenes
15
Selective or Differential

EMB
Both
16
Selective or Differential

MacConkey Agar
Both
17
It inhibits the growth of gram-positive bacteria
Methylene Blue
18
True or False

The gram-negative bacteria enhances the cell nucleus of bacteria to protect bacteria from the dye in the EMB plates
False
19
It enters the cell of gram positive bacteria and kills it
Dye
20
Has the ability of a gram negative bacterium to metabolize lactose
MacConkey Agar
21
Frequently used in culture testing
MacConkey Agar
22
What are the contents of MacConkey Agar that inhibits the growth of most gram-positive
crystal violet dye
bile salts
lactose
neutral red indicator
23
the food source of MacConkey Agar
Sugar
24
color of neutral solution pH indicator
yellow
25
what is the color if it is acidic environment for MacConkey Agar
pink to red
26
They are fermented by lactose, producing acidic and form pink to red in color
MacConkey Agar
Escherichia coli
Enterobacter aerogenes
27
They are Not fermented by lactose and form a off-white colonies
Salmonella
Shigella
Pseudomonas
28
if the acid produce by lactose which means the bacteria utilizes lactose called
Carbon source
29
It is a suitable medium for testing susceptibility of microorganism
Mueller Hinton Media
30
They are essential for testing susceptibility of microorganism of the Mueller Hinton Media
Sulfonamides
Trimethoprim
31
The trademark of accomplishment in microbiology laboratory
Sterility
32
Which is true about Sterilization?
A. To obtain precise and accurate results proper protocols which include antiseptic technique to be employed
B. The process of rendering a medium or material free of limited forms of life
C. NOTA
D. AOTA
C. NOTA
33
Most useful approach in sterilizing by exposing it to steam
Moist heat by autoclaving (Steam under pressure)
34
is the process of rendering a medium or material free of all forms of life
Sterilization
35
Type of media that can't withstand high temperature
Culture Media Filter
36
It is recommended for glassware, since it moist heat steam tends to etch and leaves glassware damp
Dry Heat
37
The temperature of autoclave
121 C Deg, 15 lbs psi
38
Time duration of autoclave
15-30 minutes
39
Endospore will not survive if it takes longer in what specific time?
12 minutes
40
Glassware be place at the over and set between____ and not exceed for 180 C Deg
160-170 C Deg for 2 hours
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