1it accomplished by growing or culturing them on the surface of solid nutrient media
Media Isolation
2what is the content of Normal mixture of Medium
Protein digest (peptone and tryptone)
Inorganic salt
1.5% agar
3True or False
The standard or general purpose of media is to support the growth of wide variety of bacteria?
True
4It contains high concentration of Sodium Chloride
Mannitol Salt Agar
5A. The 1st Statement is correct. The 2nd Statement is incorrect.
B. The 1st Statement is incorrect. The 2nd Statement is correct.
C. Both Statements are correct.
D. Both Statements are incorrect.
Mannitol salt agar contains a high concentration of sodium chloride. It inhibits the growth of most organisms but does not permit staphylococci to grow
A. The 1st statement is correct. The 2nd statement is incorrect.
6Which is true about Differential Media?
A. It compound to allow group for microorganism to be visually distinguish by the appearance of the colony or the surrounding media
B. Usually on the basis of some biochemical difference between the one group
C. Used to distinguish between bacterial cultures based on their biochemical structure
D. AOTA
A. It compound to allow group for microorganism to be visually distinguish by the appearance of the colony or the surrounding media
7Food source of blood agar
Blood
8Selective or Differential
Blood Agar
Selective
9It allows bacteria to be distinguished by the type of hemolysis produced
Blood Agar
10It is selective for gram-negative coliforms. This media can differentiate lactose fermenting and non-lactose fermenting bacteria
Eosin-methylene blue agar
11It differentiates between two major coliforms which are E. Coli and Enterobacter aerogenes
Eosin-methylene blue agar or EMB
12Selective or Differential
MHA
Selective
13A small, green-metallic sheen
E. Coli
14A large, rose color can be found inside the stomach
Enterobacter aerogenes
15Selective or Differential
EMB
Both
16Selective or Differential
MacConkey Agar
Both
17It inhibits the growth of gram-positive bacteria
Methylene Blue
18 True or False
The gram-negative bacteria enhances the cell nucleus of bacteria to protect bacteria from the dye in the EMB plates
False
19It enters the cell of gram positive bacteria and kills it
Dye
20Has the ability of a gram negative bacterium to metabolize lactose
MacConkey Agar
21Frequently used in culture testing
MacConkey Agar
22What are the contents of MacConkey Agar that inhibits the growth of most gram-positive
crystal violet dye
bile salts
lactose
neutral red indicator
23the food source of MacConkey Agar
Sugar
24color of neutral solution pH indicator
yellow
25what is the color if it is acidic environment for MacConkey Agar
pink to red
26They are fermented by lactose, producing acidic and form pink to red in color
MacConkey Agar
Escherichia coli
Enterobacter aerogenes
27They are Not fermented by lactose and form a off-white colonies
Salmonella
Shigella
Pseudomonas
28if the acid produce by lactose which means the bacteria utilizes lactose called
Carbon source
29It is a suitable medium for testing susceptibility of microorganism
Mueller Hinton Media
30They are essential for testing susceptibility of microorganism of the Mueller Hinton Media
Sulfonamides
Trimethoprim
31The trademark of accomplishment in microbiology laboratory
Sterility
32Which is true about Sterilization?
A. To obtain precise and accurate results proper protocols which include antiseptic technique to be employed
B. The process of rendering a medium or material free of limited forms of life
C. NOTA
D. AOTA
C. NOTA
33Most useful approach in sterilizing by exposing it to steam
Moist heat by autoclaving (Steam under pressure)
34is the process of rendering a medium or material free of all forms of life
Sterilization
35Type of media that can't withstand high temperature
Culture Media Filter
36It is recommended for glassware, since it moist heat steam tends to etch and leaves glassware damp
Dry Heat
37The temperature of autoclave
121 C Deg, 15 lbs psi
38Time duration of autoclave
15-30 minutes
39Endospore will not survive if it takes longer in what specific time?
12 minutes
40Glassware be place at the over and set between____ and not exceed for 180 C Deg
160-170 C Deg for 2 hours