Different types of hemagglutinations
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1
What are the different types of hemagglutinations?
Direct agglutination & hemagglutination
Indirect agglutination
Reserve passive agglutination
Agglutination inhibition
Hemagglutination inhibition
Indirect agglutination
Reserve passive agglutination
Agglutination inhibition
Hemagglutination inhibition
2
What is direct agglutination?
occurs when antigens are found naturally on a particle
3
What is the principle of direct agglutination?
antigen is naturally found on a particle
4
What are the results of direct agglutination?
agglutination indicates the presence of patient antibody.
5
What is hemagglutination?
an agglutination reaction involving RBCs
6
What type of rxn is ABO Blood Typing ?
Hemagglutination
7
How does ABO blood typing work?
Patient RBCs are mixed with an antiserum of the IgM type.
8
What does ABO Blood Typing determine?
Presence or absence of the A and B antigens
9
What are the different gradings in ABO Blood Typing?
Negative, grade 1, grade 2, grade 3, and grade 4
10
What is indirect (passive) agglutination?
employs particles that are COATED WITH ANTIGENS not normally found on their surfaces.
11
What are the results for indirect (passive) agglutination?
Agglutination indicated the presence of patient antibody
12
What type of agglutination is done prior to blood transfusions?
indirect (passive agglutination)
13
What is the principle for Reverse passive agglutination?
particles coated with reagent antibody
14
what are the results for reverse passive agglutination?
agglutination indicates presence of patient antibody
15
What is the principle of agglutination inhibition?
Haptens attached to carrier particles. Particles compete with patient antigens for a limited number to antibody sites.
16
What are the results of agglutination inhibition?
Lack of agglutination is a positive test, indicating the presence of patient antigen
17
What is the principle of hemagglutination inhibition?
Red blood cells spontaneously agglutinate if viral particles are present.
18
What is the result of hemagglutination inhibition?
lack of agglutination is a POSITIVE test - indicating presence of patient ANTIBODY
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