Different types of hemagglutinations
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1
What are the different types of hemagglutinations?
Direct agglutination & hemagglutination
Indirect agglutination
Reserve passive agglutination
Agglutination inhibition
Hemagglutination inhibition
Indirect agglutination
Reserve passive agglutination
Agglutination inhibition
Hemagglutination inhibition
2
What is direct agglutination?
occurs when antigens are found naturally on a particle
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What is the principle of direct agglutination?
antigen is naturally found on a particle
4
What are the results of direct agglutination?
agglutination indicates the presence of patient antibody.
5
What is hemagglutination?
an agglutination reaction involving RBCs
6
What type of rxn is ABO Blood Typing ?
Hemagglutination
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How does ABO blood typing work?
Patient RBCs are mixed with an antiserum of the IgM type.
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What does ABO Blood Typing determine?
Presence or absence of the A and B antigens
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What are the different gradings in ABO Blood Typing?
Negative, grade 1, grade 2, grade 3, and grade 4
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What is indirect (passive) agglutination?
employs particles that are COATED WITH ANTIGENS not normally found on their surfaces.
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What are the results for indirect (passive) agglutination?
Agglutination indicated the presence of patient antibody
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What type of agglutination is done prior to blood transfusions?
indirect (passive agglutination)
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What is the principle for Reverse passive agglutination?
particles coated with reagent antibody
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what are the results for reverse passive agglutination?
agglutination indicates presence of patient antibody
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What is the principle of agglutination inhibition?
Haptens attached to carrier particles. Particles compete with patient antigens for a limited number to antibody sites.
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What are the results of agglutination inhibition?
Lack of agglutination is a positive test, indicating the presence of patient antigen
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What is the principle of hemagglutination inhibition?
Red blood cells spontaneously agglutinate if viral particles are present.
18
What is the result of hemagglutination inhibition?
lack of agglutination is a POSITIVE test - indicating presence of patient ANTIBODY
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