1What is placed on a thin piece of glass to hold a specimen for a light microscope?
A slide
2What is sometimes needed if a specimen is colourless to help identify organelles?
A stain
3What is placed on top of a slide to protect the lens?
A cover slip
4Where is a microscope slide mounted?
On the stage
5What part of a light microscope shines constant light on the slide?
The lamp
6Which lens is found closest to the specimen on a microscope?
The objective lens
7What further magnifies the image after the objective lens?
The eyepiece lens
8What is magnification defined as?
The degree to which the size of an image is larger than the real object
9What is the formula for the magnification of an object?
Image size/object size
10What is the formula for the total magnification of a microscope?
Magnification of the eyepiece lens x magnification of the objective lens
11What is resolution defined as?
The ability to distinguish between 2 points that are close to each other
12Between what two values must the number be when using standard form to compare sizes?
Between 1 and 10
13What are some advantages of light microscopes?
- Relatively cheap
- Can be used in the field
- Does not require specialist training
- Can look at living specimens
14What are some disadvantages of light microscopes?
- Low resolution
- Low magnification strength
- Staining required for some organelles
15What is the resolution of a standard light microscope?
200nm
16What is the maximum magnification strength of a light microscope?
x1500
17What do electron microscopes use instead of light to form images?
Electrons
18Which electron microscope creates 3D images at a lower resolution?
Scanning Electron Microscope (SEM)
19What is the resolution and magnification of a Scanning Electron Microscope (SEM)?
10nm resolution and x100,000 magnification
20Which electron microscope creates 2D images detailing organelles at a much higher resolution?
Transmission Electron Microscope (TEM)
21What is the resolution and magnification of a Transmission Electron Microscope (TEM)?
0.2nm resolution and x500,000 magnification
22What are some disadvantages of electron microscopes?
- Extremely expensive
- Very large
- Requires specialist training
- Samples must be in a special vacuum
23Which cell structure contains genetic material and codes for a particular protein?
Nucleus
24What membrane encloses the nucleus?
Nuclear membrane
25What is the liquid substance in plant and animal cells where chemical reactions occur?
Cytoplasm
26What are biological catalysts that speed up the rate of reaction called?
Enzymes
27What structure contains receptor molecules to identify and selectively control what enters and leaves the cell?
Cell membrane
28Where do aerobic respiration reactions occur, providing energy for the cell?
Mitochondria
29Where does protein synthesis occur in a cell?
Ribosomes
30On what structure are ribosomes often found?
Rough endoplasmic reticulum
31Where does photosynthesis take place in plant cells?
Chloroplasts
32What pigment found in chloroplasts makes plants green and harvests light?
Chlorophyll
33What does the permanent vacuole contain?
Cell sap
34What structure helps improve a plant cell's rigidity?
Permanent vacuole
35What is the plant cell wall made from?
Cellulose
36What compound makes up the cell wall in prokaryotic cells like bacteria?
Peptidoglycan
37How is DNA structured in prokaryotic cells?
As a single circular strand of DNA
38What are the small rings of DNA found in prokaryotic cells called?
Plasmids
39What does DNA stand for?
Deoxyribonucleic acid
40What is the physical structure of DNA?
A double helix made from 2 strands that have twisted around each other
41What are the individual molecules called that join up to make a long DNA strand?
Nucleotides
42What are the three components of a nucleotide?
- One sugar molecule
- One phosphate group
- One of the four organic bases
43What are the four organic bases in DNA?
A, C, G, T
44Which base does cytosine (C) always pair with?
Guanine (G)
45Which base does adenine (A) always pair with?
Thymine (T)
46What does each group of three bases code for?
An amino acid
47What are structures made up of long molecules of DNA called?
Chromosomes
48Why is DNA unable to leave the nucleus during protein synthesis?
It is too large
49What does mRNA stand for?
Messenger RNA
50What process creates a template strand of mRNA from DNA?
Transcription
51Where does the template strand of mRNA move to after leaving the nucleus?
The cytoplasm
52What process involves bases on mRNA being read in threes at the ribosomes to code for amino acids?
Translation
53What brings corresponding amino acids to the ribosomes during protein synthesis?
Carrier molecules
54What shape does a complete protein form when its amino acid chain folds?
A unique 3D structure
55What are biological catalysts that increase the rate of reaction without being used up?
Enzymes
56What uniquely shaped region on an enzyme is where the substrate binds?
Active site
57What hypothesis describes how an enzyme and substrate specifically fit together like a lock and key?
Lock and Key Hypothesis
58What is formed when a substrate binds to an enzyme's complementary active site?
An enzyme-substrate complex
59What is the approximate optimum temperature for human enzymes?
37 degrees celsius
60What happens to an enzyme when it becomes too hot and its bonds break?
It is denatured
61What is the optimum pH for most enzymes?
7
62What supplies ATP to cells through an exothermic reaction occurring in every living cell?
Respiration
63Which type of respiration uses oxygen and yields the most energy?
Aerobic respiration
64Where do most reactions of aerobic respiration occur?
Mitochondria
65What is the word equation for aerobic respiration?
Glucose + oxygen -> carbon dioxide + water
66When is anaerobic respiration used as a last resort in animals?
When there is not enough oxygen during a sprint
67What is the product of anaerobic respiration in animals?
Lactic acid
68What is anaerobic respiration called in plant and yeast cells?
Fermentation
69What are the products of fermentation in plant and yeast cells?
Ethanol + carbon dioxide
70What industrial uses does fermentation have?
Making bread and alcoholic drinks
71What type of polymers are carbohydrates like starch made from?
Simple sugars or monomers
72What type of enzymes break down carbohydrates?
Carbohydrase enzymes
73Name an example of a carbohydrase enzyme that breaks down starch.
Amylase
74Starch is broken down into which simple sugar by amylase?
Maltose
75What enzyme synthesises glycogen from glucose?
Glycogen synthase enzyme
76What is glycogen used for in the body?
As an energy store
77What polymers are proteins made of?
Amino acids
78What enzymes break down proteins in the stomach and small intestine?
Protease enzymes
79What enzymes break down lipids?
Lipase enzymes
80What are the breakdown products of lipids?
Glycerol and 3 fatty acids
81Where is bile made?
Liver
82What is the function of bile in digestion?
Emulsifies fat into small droplets to make it easier for lipase enzymes to work
83What process makes glucose from sunlight in the leaves of a plant?
Photosynthesis
84Is photosynthesis an exothermic or endothermic reaction?
Endothermic
85Where is energy transferred from during photosynthesis?
The environment to the chloroplasts by light
86What is the word equation for photosynthesis?
Carbon dioxide + water -> glucose + oxygen
87What apparatus is often used with pondweed in water to measure oxygen production during photosynthesis?
A capillary tube attached to a syringe
88What happens to the rate of photosynthesis as temperature increases up to a certain point?
It increases
89What solution turns blue-black in the presence of starch during leaf testing?
Iodine solution
90What law states that light intensity is inversely proportional to the square of the distance from the light source?
Inverse square law
91What is the formula for light intensity according to the inverse square law?
1/distance2
92What is an environmental condition that, in low levels, restricts any increase in the rate of photosynthesis called?
A limiting factor
93What units is light intensity measured in on photosynthesis graphs?
Lux