1Definition of cytochemistry in the study of cells refers to
Study of chemical constituents of cells
2Diagnostic utility of cytochemical techniques is particularly useful for
Acute leukemias
3Specimen types routinely used for cytochemical evaluations include
Bone marrow, lymph nodes, peripheral blood
4Smear freshness requirement for enzymatic cytochemical techniques is
Fresh smears
5Storage stability condition for nonenzymatic techniques (like PAS/SBB) is
May remain stable for months if stored at room temp
6Primary cellular site where Myeloperoxidase (MPO) enzyme is located includes
Primary granules of neutrophils and eosinophils, mono (to a certain extent)
7Primary diagnostic application of Myeloperoxidase (MPO) is
Used in differentiating blasts of AML from those of ALL
8Diagnostic significance of a positive (+) peroxidase stain regarding ALL is
Rules out ALL
9Cell types demonstrating POSITIVE Myeloperoxidase (MPO) reactivity include
Neutrophilic granulocytes (except normal blasts), Auer rods, leukemic blasts in FAB M1, M2, and M3, eosinophils
10Cell types demonstrating WEAKLY POSITIVE or NEGATIVE Myeloperoxidase (MPO) reactivity include
Monocytes
11Cell types demonstrating NEGATIVE Myeloperoxidase (MPO) reactivity include
Myeloblasts, basophils, lymphocytic cell series, erythrocytic cell series
12General relationship of Sudan Black B (SBB) reactions compared to MPO is
Reactions parallel those of the MPO's (in most cases)
13Specific cellular components stained by Sudan Black B (SBB) include
Sterols, neutral fats, phospholipids (found in primary and secondary granules of neutrophils and lysosomal granules of monocytes)
14Cell types demonstrating POSITIVE Sudan Black B (SBB) reactivity include
Promyelocyte, myelocyte, metamyelocytes, bands, and segmented neutro. (STRONGLY POSITIVE), leukemic blasts, Auer rods, eosinophils
15Cell types demonstrating WEAKLY POSITIVE or NEGATIVE Sudan Black B (SBB) reactivity include
Myeloblasts, monocytic cells
16Cell types demonstrating NEGATIVE Sudan Black B (SBB) reactivity include
Lymphocytes and its precursors, megakaryocytes and platelets, erythrocytes
17Primary diagnostic purpose of esterase staining in hematology is
Differentiate acute granulocytic leukemias from monocytic leukemias
18Substrates used for nonspecific esterase (NSE) testing are
alpha-naphthyl acetate and alpha-naphthyl butyrate
19Substrate used for specific esterase testing is
Naphthol AS-D Chloroacetate
20Cell types demonstrating POSITIVE alpha-naphthyl acetate esterase (NSE) reaction include
Monocytes (strong positive reaction), positive for other certain cell types
21Cell types demonstrating WEAKLY POSITIVE or NEGATIVE alpha-naphthyl acetate esterase (NSE) reaction include
Granulocytes, lymphoid cells (lymphocytic cell lines)
22Reactivity of monocytes with alpha-naphthyl acetate esterase in the presence of NaF is
NEGATIVE (with NaF inhibition)
23Cell types demonstrating POSITIVE alpha-naphthyl butyrate esterase (NSE) reaction include
Monocytes, positive for other certain cell types
24Cell types demonstrating WEAKLY POSITIVE or NEGATIVE alpha-naphthyl butyrate esterase (NSE) reaction include
Granulocytes, lymphoid cells (lymphocytic cell lines)
25Reactivity of monocytes with alpha-naphthyl butyrate esterase in the presence of NaF is
NEGATIVE (with NaF inhibition)
26Cell types demonstrating POSITIVE Naphthol AS-D Chloroacetate esterase reaction include
Promyelocyte, myelocyte, metamyelocyte, bands, seg. neutro, leukemic myeloblasts, Auer rods
27Cell types demonstrating WEAKLY POSITIVE or NEGATIVE Naphthol AS-D Chloroacetate esterase reaction include
Monocytic cells
28Cell types demonstrating NEGATIVE Naphthol AS-D Chloroacetate esterase reaction include
Myeloblasts (variable), monoblasts, promonocytes and monocytes
29Target cellular substance stained by Periodic Acid-Schiff (PAS) reaction is
Glycogen
30Diagnostic utility of Periodic Acid-Schiff (PAS) reaction in leukemia classification is
Useful in identifying FAB M6 leukemia
31Reactivity of RBCs in erythroleukemia (FAB M6) with Periodic Acid-Schiff (PAS) reaction is
STRONGLY POSITIVE
32Reactivity of normal erythroid precursors with Periodic Acid-Schiff (PAS) reaction is
PAS negative
33Biological classification of Terminal Deoxynucleotidyl Transferase (TdT) is
Enzyme marker for primitive lymphoid cells
34Percentage of ALL patients demonstrating strong TdT activity is
Approximately 90% of patients with ALL
35Clinical utility of Terminal Deoxynucleotidyl Transferase (TdT) in CML evaluation is
Helpful in the recognition of the "lymphoblastic transformation" of CML