Pyruvate Oxidation and Citric Acid Cycle

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1
Pyruvate Oxidation
Conversion of pyruvate to acetyl-CoA, producing CO₂ and NADH in mitochondrial matrix.
2
Acetyl-CoA
2-carbon molecule entering the Citric Acid Cycle.
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Citric Acid Cycle (CAC)
Series of reactions oxidizing acetyl-CoA to CO₂ and producing NADH, FADH₂, and ATP.
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Location of CAC
Mitochondrial matrix.
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Oxaloacetate (OAA)
4-carbon molecule that combines with acetyl-CoA to start the cycle.
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Citrate
6-carbon compound formed from OAA + acetyl-CoA.
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Decarboxylation
Removal of CO₂ during pyruvate and cycle reactions.
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Per Cycle Products
3 NADH, 1 FADH₂, 1 ATP (2 CO₂).
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Per Glucose (2 turns)
6 NADH, 2 FADH₂, 2 ATP, 4 CO₂.
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Main Function
Generate electron carriers for oxidative phosphorylation.
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Substrate-Level Phosphorylation in CAC
Direct formation of ATP from ADP and substrate.
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Regulation
Controlled by availability of acetyl-CoA and feedback from NADH/ATP levels.
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Mitochondrial Matrix
Inner space where CAC enzymes operate.
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