Medicine Y3 S1: Microbiology
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Name of organism ?
Mycobacterium tuberculosis
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Which stain is used ?
Ziehl-Neelsen (ZN) stain
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Organism ?
Cryptococcus neoformans
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Stain ?
India ink stain
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Budding yeast cells surrounded by a large gelatinous capsule
Cryptococcus neoformans
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Name of test ?
Latex Aggulitnation test
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Which one is +ve and which one is -ve ?
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Name of test ?
Latex Aggulitnation test
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Which one is +ve and which one is -ve ?
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Latex Aggulitnation test is done directly/Indirectly on CSF ?
Directly
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What test detects Capsular Ag in CSF
Latex Aggulitnation test
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What are the capsulated organisms that can be found in CSF in a case of septic meningitis ?
1 Meningococci (N.meningitidis)
2 H.influenzae
3 Pneumococci (Streptococcus pneumoniae)
4 Cryptococcus neoformans
2 H.influenzae
3 Pneumococci (Streptococcus pneumoniae)
4 Cryptococcus neoformans
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What reaction can be used to detect capsulated bacteria in CSF by appearance microscopically ?
Quellung reaction (swollen capsules)
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Quellung reaction
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How does Quellung reaction work ?
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What are the organisms that can be detected by Quellung reaction from a CSF sample ?
1 Meningococci (N.meningitidis)
2 H.influenzae
3 Pneumococci (Str.pneumoniae)
(i.e., they are capsulated)
2 H.influenzae
3 Pneumococci (Str.pneumoniae)
(i.e., they are capsulated)
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Nutrient agar
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Nutrient agar
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S.aureus on NA
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Pseudomonas aeruginosa + its green exopigment on NA
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Pseudomonas aeruginosa + its green exopigment on NA
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Pseudomonas aeruginosa + its green exopigment on NA
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Pseudomonas aeruginosa + its green exopigment on NA
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β-Haemolysis on blood agar
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α-Haemolysis
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α-Haemolysis on blood agar + Optochin sensitive
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Organism ?
Streptococcus pneumoniae (Pneumococci)
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γ-Haemolysis (Non-haemolysis) on blood agar
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Organism/s ?
1 Haemophilus
2 Neisseria
2 Neisseria
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Enriched and SELECTIVE
Modified Thayer Martin (MTM) medium
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Enriched and SELECTIVE for what organism ?
Neisseria (MTM medium)
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Candle jar
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For what type of bacteria ? + Example
Candle jar: for Capnophilic (e.g., Neisseria)
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MacConkey's agar
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+ Example
Lactose fermentation of MCA (e.g., E.coli)
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NLF on MCA
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What is this medium and used for which organism ?
Lowenstein Jensen (LJ) medium for Mycobacterium tuberculosis
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What Medium and for what kind of bacteria + Example
Robertson's cooked meat broth
For Anaerobic bacteria (e.g., Clostridium)
For Anaerobic bacteria (e.g., Clostridium)
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What Medium and for what kind of bacteria + Example
Deep agar
For Anaerobes (e.g., Clostridium)
For Anaerobes (e.g., Clostridium)
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Fungal
Candida on Saboraud's Destrose agar (SDA)
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Test and organism
Neisseria meningitidis is Oxidase-+ve
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Test and organism
Candida albicans in Germ tube test at 37C
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Antibiotic sensitivity test: Disc diffusion method
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