WBC Count
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1
WBC functions
soldiers of the body
2
two groups of WBCs
granulocytes
agranulocytes
agranulocytes
3
these are granulocytes
neutrophils, eosinophils, basophils
4
these are agranulocytes
monocytes
lymphocytes
lymphocytes
5
number of all types of WBCs
total leukocyte count
6
reference range for adult male and females
4.5 - 11.5 x 10^9/L
7
significance of performing manual WBC count
1. check validity of electronic methods during calibration
2. check valiidity of results from automated machines in px with leukopenia, leukemia, or thrombocytopenia
3. faulty machines
2. check valiidity of results from automated machines in px with leukopenia, leukemia, or thrombocytopenia
3. faulty machines
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condition with low WBC count
leukopenia
9
automated machines have the tendencies to
1. identify platelets as WBCs
2. automated results in platelet clumping is usually increased WBC count and decreased platelets
2. automated results in platelet clumping is usually increased WBC count and decreased platelets
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platelet clumps are mistaken as WBC
increased WBC count
11
platelet clumps cant be counted by automated machine leads to
decreased platelet count
12
what pipette is used in the dilution of sample
thoma pipette
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up to what mark is the blood aspirated in the thoma pipette
0.5 mark
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condition with increased WBC
leukocytosis
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what to do with blood aspiration if px has leukocytosis
1. aspirate up to 0.2 mark
2. use RBC thoma pipette; aspirate up to 0.5 mark and dilute to 101 mark
2. use RBC thoma pipette; aspirate up to 0.5 mark and dilute to 101 mark
16
what to do with blood aspiration if px has leukopenia
1. aspirate up to 1 mark
2. increase number of squares counted to all 9 squares
2. increase number of squares counted to all 9 squares
17
when adjusting volume of blood aspirated, volume of diluent should also be adjusted
- true
- false
18
how do we remove/lyse RBCs to prevent them from concealing WBCs
use slightly acidic/hypotonic solution
19
WBC diluting fluids
1. 2-3% glacial acetic acid
2. turks solution
3. 1% hydrochloric acid (HCl)
2. turks solution
3. 1% hydrochloric acid (HCl)
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composition of 2-3% glacial acetic acid
glacial acetic acid
distilled water
1% aqueous gentian violet
distilled water
1% aqueous gentian violet
21
composition of turks solution
glacial acetic acid
distilled water
methyl violet
distilled water
methyl violet
22
under normal conditions, which squares of the hemocytometer should be counted
4 large corner squares
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total volume of solution in the bulb / volume of blood aspirated
dilution factor formula
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what is the automatic amount of the dilution factor if it is not given/mentioned
20
25
diluting fluid only lyse mature RBCs and leave nucleated RBCs intact
- true
- false
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NRBCs and WBCs are indistinguishabe under the microscope
- true
- false
27
nucleated RBCs can falsely [ ] WBC count
increase
28
if [ ] NRBCs are found during differential count, corrected WBC count should be calculated
5 or more
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uncorrected WBC count x 100 divided by number of NRBCs + 100
corrected WBC count formula
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conditions with leukocytosis
leukemia
leukomoid reaction
hemorrhage
bacterial infection
viral infection
exercise
leukomoid reaction
hemorrhage
bacterial infection
viral infection
exercise
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what WBC increases during bacterial infection
neutrophils
32
what WBC increases during viral infection
lymphocytes and monocytes
33
what WBC increases during parasitic infections
eosinophils
34
during exercise, WBCs move from [ ] pool to the [ ] pool
marginal ; circulating
35
conditions with leukopenia
bone marrow injury
splenomegaly
autoimmune disorders
overwhelming infection and inflammation
splenomegaly
autoimmune disorders
overwhelming infection and inflammation
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