CHEM 101 Test 2 Microscope Chapter

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1
Real image vs Virtual image?
Real image is when rays converge while virtual image is when rays diverge. Virtual image would be mirror reflection. Real image would be cinema screen.
2
What are the microscopes discussed in class?
Stereomicroscope, Biological scope, Polarizing scope, Comparison scope, Fluorescence scope, Electron microscope.
3
What is Ocular lens? Objective lens?
Magnify the image produced by the objective lens. Directly observe the object the microscope user is examining.
4
Describe stereomicroscope...
2 objective lenses, low mag, used for preparing samples
5
Describe biological microscope...
Common microscope, X-Y stage
6
Describe polarizing microscope...
Bertrand lens. Used to determine the difference between glass and any other similar substance.
7
Filters of the polarizing microscope...
Polarizer - first filter beneath specimen, Analyzer - 2nd filter above specimen, Extinction - No light passing through 2nd filter.
8
Describe Comparison scope...
Used for ballistics and hair, 2 stages.
9
Describe the Fluorescence scope...
UV light source, Quartz lenses, Excitation filter to excite sample.
10
Electron microscope?
Electrons are shot down through a tunnel onto sample and create image. High mag and resolution.
11
Define parfocal...
All other objective lens are in focus.
12
How to calculate total mag?
objective*ocular
13
How to calculate max useful mag?
1000*normal aperature
14
What is the max mag of any visible light microscope?
1500x
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