Respiration experiments
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1
What can you investigate using yeast?
factors affecting respiration
2
What type of organism is yeast?
single-celled
3
What does yeast respire both aerobically and anaerobically?
sugars
4
What indicator shows aerobic respiration rate?
redox indicator dye
5
What color does the dye change to?
colorless
6
What is the first step in aerobic respiration experiments?
put yeast in a test tube
7
What temperature should you set the water bath to?
40°C, 70°C, or 25°C
8
What substance is added to start aerobic respiration?
glucose
9
How do you measure the rate of respiration?
time for dye to turn colorless
10
What does the mean rate of respiration equal?
1 ÷ mean time for color change
11
What gas does yeast produce during anaerobic respiration?
carbon dioxide
12
What apparatus is used for anaerobic respiration?
syringe or gas syringe
13
What is used to exclude oxygen in anaerobic experiments?
liquid paraffin
14
What is measured during anaerobic respiration experiments?
volume of CO₂ released
15
What is a respirometer used to measure?
rate of oxygen consumption
16
What solution absorbs CO₂ in respirometers?
potassium hydroxide
17
What happens to the manometer fluid if oxygen is used?
moves toward the test tube
18
What is the equation for the rate of respiration in respirometers?
volume of O₂ ÷ time
19
What variables should be controlled in respiration experiments?
temperature, pH, substrate concentration
20
What should be changed one at a time?
independent variable
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