Common Enzymes and Characteristics

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1
Purpose of hydrolases?
catalyze hydrolytic cleavage rx
2
Two common types of hydrolases:
(nucleases and proteases are a type)
3
Purpose of proteases?
break down proteins by hydrolyzing bonds between amino acids
4
Purpose of synthases?
synthesize molecules in anabolic reactions by condensing two smaller molecues together
5
Purpose of Ligases?
join together two molecules in an energy dependent way
6
One ex of a ligase?
-DNA ligase
7
What do isomerases do?
catalyze the rearrangement of bonds within a single molecule
8
What do polymerases do? provide an ex
catalyze polymerization of reactions, ex DNA and RNA
9
Purpose of Kinases?
catalyze additon of phosphate groups to molecules
10
What do protein kinases do?
attach phosphate groups to proteins
11
Purpose of phosphatases?
-catalaze hydrolytic removal of phosphate group from a molecule
12
What are oxido-reductases? three common names
enzymes that catalyze rx here one molecule is oxidized while the other is reduced (oxidases, reductases, hehydrogenases)
13
Purpose of ATPases?
hydrolyze ATP, found on many proteins
14
In what processes would ATPase be found?
-motor proteins (myosin)
-transport proteins (Na-K pump)
15
What do GTPases do?
-hydrolyze GTP
16
What factors influence enzyme activity?
PH, temp, substrate concentration, regulation
17
Role of disulfide isomerases?
e key players in ensuring that enzymes fold properly and maintain their structural integrity (stabilize active sites, help revent misfolding)
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