BIOL 150 EXAM 5

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1
Eukaryotic Cell Cycle
Every cell orginates from another cell
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cell cycle
the highly ordered sequence of events that takes place in a cell, resulting in division of
the nucleus and the formation of two genetically identical daughter cells
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Humans have how many chromosomes?
46 (23 pairs)
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Autosomes in humans
22 pairs
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sex chromosomes
X and Y chromosomes.
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Polidy
number of sets of chromosomes
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Diploid
2 sets of chromosomes
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Haploid
An organism or cell having only one complete set of chromosomes.
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Homologs
Members of a pair of chromosomes
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Autosomes
Any chromosome that is not a sex chromosome
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Sister chromatids
Replicated forms of a chromosome joined together by the centromere
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homologous chromosomes
Pair of chromosomes that are the same size, same appearance and same genes.
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Stages of the cell cycle
G1, S, G2, M, G0
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G1
First Gap
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S
synthesis of DNA
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G2
second gap
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M
mitosis and cytokinesis
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G0
substitutes for G1 for cells postponing division or never dividing again
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Phases of interphase
G1, S, G2
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G1 Phase
stage of interphase in which cell grows and performs its normal functions, 46 chromosomes
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S Phase
Chromosome is replicated to form Sister chromatids
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G2
the cell synthesizes proteins for cell division
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M Phase
mitosis and cytokinesis
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Mitosis
Separation of sister chromatids
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Cytokinesis
division of the cytoplasm
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Cyclin or Cyclin dependent Kinase are responsible for
advancing cells through phases of cell cycle
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cell cycle checkpoints
critical point in cell cycle that is regulated
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3 checkpoints in eukaryotes
G1, G2, Metaphase
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Checkpoint proteins
sensors that determine if the cell is in proper condition to divide
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GI checkpoint
Determines if conditions are favorable for cell division by sensing DNA damage
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G2 checkpoint
Ensures that all DNA is replicated and monitors proteins needed to advance to M
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M checkpoint
cell monitors spindle formation and attachment to kinetochores
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miotic cell division
produces two daughter cells from an original somatic cell, asexual reproduction
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Mitosis steps
prophase, prometaphase, metaphase, anaphase, telophase, cytokinesis
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miotic spindle
a football-shaped structure of microtubules that guides the separation of the two sets of daughter chromosomes
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Prophase
Joined sister chromatids formed and nuclear membrane disassociates
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Prometaphase
Nuclear envelope breaks down and Miotic spindle is formed
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Metaphase
Pairs of sister chromatids are aligned along a plane halfway b/t poles called the metaphase plate
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Anaphase
the third phase of mitosis, during which the chromosome pairs separate and move toward opposite poles
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Telophase
chromosomes move to opposite ends of the cell and two nuclei are formed.
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cytokinesis in plant cells
cell plate forms
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cytokinesis in animals
Actin helps the cleavage furrow form
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Meiosis
The process by which haploid cells are produced from a cell that was originally a diploid
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How many rounds of division are in meiosis?
two
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Bivalet/tetrad
Homologous pairs of sister chromatids associate with each other through synapsis
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Synaptonemal complex
proteins that hold together homologous chromosomes
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crossing over
Process in which homologous chromosomes exchange portions of their chromatids during meiosis.
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Chiasma
arms of the chromosomes tend to separate but remain adhered at a crossover site
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Meiosis 1 stages
prophase 1, metaphase 1, anaphase 1, telophase 1 and cytokinesis
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Prophase 1
Crossing over occurs
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Prometaphase 1
spindles attach to kinetochores on chromosomes
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Metaphase 1
Paired homologous chromosomes line up across the center of the cell
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# of different random alignments in metaphase 1
2^n n= # of chromosome per set
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Anaphase 1
Homologous chromosomes separate
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Telophase 1
A nuclear membrane forms around each cluster of chromosomes on each pole
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End of meiosis 1
two haploid cells, with no pairs of homologous change
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Meiosis II is similar to mitosis in that
sister chromatids separate. However it is done during anaphase II
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sexual reproduction
requires fertilization event in which two haploid gametes unite to create a diploid cell called a zygote
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asexual reproduction
A reproductive process that involves only one parent and produces offspring that are identical to the parent.
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Disadvantages of sexual reproduction
It costs energy
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Advantages of sexual reproduction
genetic variation
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Diploid dominant
Mostly animal species and are specialized cells
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haploid-dominant
Many fungi and some protists
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Inheritance
The acquisition of traits by their transmission from parents to offspring
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blending inheritance
1700s hypothesis that suggested genetic material of two parents blended together in offspring
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particulate inheritance
19th century hypothesis that determined hereditary traits are transmitted in discrete units or particles from one generation to the next
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Gregor Mendel
Priest that experimented with pea plant characters and traits
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Advantages properties of the garden pea
Genetic variation, self fertilization, hybridization
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Characters
General features of an organism
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Trait
Identifiable characteristic of an organism
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self-fertilization
fertilization in which both female and male gamete come from the same plant
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true-breeding lines
Exhibition of the same trait over several generations of self fertilization
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Procedure for Cross fertilization
1. Pollen from one plant is placed on the stigma of flower from a different plant
2. Prying open an immature flower and removing stamens
3. Take pollen from another flower's stamens and place on the stigma
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single-factor cross
Crossing with respect to a single trait
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P generation
true-breeding parents
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F1 generation
offspring of the P generation, mono hybrids if parents differ in one trait
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F2 generation
offspring of the F1 generation, recessive trait appears.
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Mendel results of single factor cross
P gen: crossed tall and dwarf plants
F1 gen: all were tall
F2 gen: dwarf trait appeared. 3/4 plants were tall, 1/4 plants were dwarf
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dominant trait
Displayed in hybrids
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Recessive trait
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Particulate factors of inheritance
Genes
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Alleles
Different forms of a gene
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Segregation of alleles
Alleles separate and move into different haploid gamete sex cells
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Mendel's law of segregation
Allen's of the same gene separate during game formation
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Only___ allele is received per gene
1
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Genotype
The genetic composition of an individual
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Homozygous
Having two identical alleles for a particular gene
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Heterozygous
An organism that has two different alleles for a trait
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Phenotype
physical characteristics of an organism
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Two factor Cross
a cross in which an experimenter follows the outcome of two different traits
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Patterns of Two-factor Cross
Two genes are liked or two gene are independent
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linked genes
Variants found together in parents that are always inherited as a unit. Produces YR or yr gametes
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independent genes
Variants are randomly distributed, produces YR, Yr, yR, or yr gametes
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Two factor cross outcome
F1: dihybrid offspring
F2: hybrids with independent assortment
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Mendel's Law of independent assortment
Alleles of different genes sort independently of each other during the process that gives rise to gametes
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When do genes not assort independently?
Allele of two genes located near each other on the same chromosome do not assort independently
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Chromosomes contain
DNA
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Genes are found in
chromosomes
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Chromosomes are passed
From parent to offspring and cell to cell
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During the formation of haploid cells, chromosomes separate ____ from each other
Independently
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locus
the physical location of a gene on a chromosome
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Probability
The chance that an event will have a particular outcome
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Product Rule
The probability that two or more independent events will occur is equal to the product of their individual probabilities
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XY system of sex determination (Mammals)
Males XY and females XY
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XO system of sex determination (Insects)
Females XX and Males X or XO
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ZW system (Birds and Fish)
Males ZZ and females ZW
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X-linked trait
trait controlled by a gene located on the X chromosome
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Males are _____ for X-linked genes
hemizygous
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Simple Mendelian Inheritance
one trait is completely dominant over the other
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Pleiotropy
The ability of a single gene to have multiple effects.
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Incomplete dominance
heterozygous phenotype that is a blend of the two homozygous phenotypes
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Codominance
A condition in which both alleles for a gene are fully expressed
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In a phenotype the environment
Provides nutrients and energy to carry out the plan
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Norm of reaction
phenotype range that individuals with a particular genotype exhibit under differing environmental conditions
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Discrete traits
clearly defined phenotypic variants
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Quantitative traits
traits that show continuous variation (most traits, i.e.: height, intelligence, athleticism)
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Polygenic
multiple genes contribute to phenotype
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