1what is the purpose of vascular tissue?
transport substances, long-distance communication
3phloem conducts
organic compounds (sugar)
4what direction does xylem move, and how?
root to leaf, evaporation on leaf causes tension that pulls water up
5xylem tissue is made of
lignin and cellulose
6where does the phloem transport to and from, and how is movement mediated?
source to sinks, mediated by hydrostatic pressure from xylem
7sieve elements
conducting cells that form a tube
8companion cells
next to sieve elements, provides metabolic support
9procambial cells
vascular stem cells
10how do leaf primordia form vasculature?
auxin sinks
11what is canalization and what leads to it?
formation of narrow channel at center of leaf; auxin/MP/PIN1 positive feedback loop
12what phenotypes are used to track circadian rhythm?
leaf movement and gene expression of luciferase, a reporter gene
13what are CAB proteins?
chlorophyll a binding proteins, fine tunes light absorption in PS 1 and 2 to improve photosynthesis efficiency
14when does CAB 1 peak?
just after dawn / early morning
15what effect does toc1 mutant have?
shortens the circadian period
16in WT, where does TOC1 peak and fall?
peaks in the late day, and falls to its lowest levels at dawn
17how can you get around genetic redundancy?
activation tagging
18what is the relationship between TOC1, CCA1, and LHY?
TOC1 promotes CCA1 and LHY, but CCA1 and LHY inhibit TOC1
19what happens if CCA1 or LHY are overexpressed?
no circadian rhythm
20CCA1 directly binds to
CAB promoter
21what trains the circadian clock?
light, perceived with PHYA
22what part of the plant is the "master" regulator of the circadian clock?
shoot apex
23vegetative to floral transition is (reversible or irreversible)
irreversible
24what are the endogenous flowering cues?
age pathway, epigenetic regulators, GA pathway
25what are the environmental flowering cues?
length of day, vernalization, average day/night temperatures
26why are there many pathways to flowering?
plasticity to ensure reproductive success
27Pfr is the _______ form
active
28Pf is the ______ form
inactive
29what is CO?
photoresponse gene regulated by circadian clock
30CO induces _______, which activates ________
FT, floral transition
31in LD conditions, CO accumulates to a ______ level during the afternoon, which leads to _______ flowering
high, accelerated
32in SD conditions, CO accumulates to a ______ level during the afternoon, which leads to ________ flowering
low, delayed
33_______ prevents CO protein accumulation in the morning by __________
PHYB, degrading
34PHYB _______ flowering in SD
suppresses
35PHYA ________ flowering in LD and ________ CO protein in the afternoon by ________
promotes, promotes, preventing degradation
36SPA is expressed in the ______ and prevents ____________ by _________
dark, CO accumulation, degradation
37spa mutant flower _______in SD because ________
early, CO is present in the dark
38how was existence of florigen shown?
grafting experiments
39what is florigen?
a signal that moves through phloem from leaves to VM and converts it to IFM
40what is FD and where is it expressed?
TF gene, only in SAM
41What does the FT-FD complex do in the center of the SAM?
activates SOC1, which promotes IFM identity
42What does the FT-FD complex do on the flanks of SAM?
activates AP1 and LFY, which promotes FM
43SOC1 induces ________ which represses _____ in the IFM
TFL1, LFY and AP1
44tfl1 mutants form a
terminal flower
45in order of the whorls, the four floral organs are
sepals, petals, stamens, carpels
46Class A genes:
AP1 and AP2; sepal and petal identity
47Class B genes:
AP3 and PI: petal and stamen identity
48Class C genes:
AG; stamen and carpel identity
49SEP proteins are _____ that physicall interact with ________
cofactors, AP3-PI, AP1 and AG proteins
50AG induces _______
genes required for flower organ formation
51AG represses ________
genes required for leaf formation
52reducing AG activity causes
formation of leaf-like structures on floral organs
53petunias have ____ class B genes
3
54sorrels have no petals in the second whorl because
class B is only expressed in the third whorl
55in male flowers there are no ______
carpels
56in female flowers there are no
stamens
57absence of _______ from the 3rd or 4th whorl can distinguish _______
class C, sexes
58tulips have ______ becase class ___ is expanded
tepals, B
59how are lacadonias different, and why?
stamen in 4th whorl, carpel in 3rd; class B expressed in 3rd and 4th whorls
60in water lilies, gene expression patterns are
fuzzy / blurred
61in gymnosperms, class C is expressed exclusively in
reproductive organs
62in gymnosperms, class B is expressed exclusively in
male reproductive organs
63in gymnosperms there is no activity for class _____ and ________
A, E
64do ferns have ABCE genes?
no
65what are the 2 types of floral variation?
variation with similar flower architecture, variation with different flower architecture
664 types of flower shapes:
radially symmetric, bilaterally symmetric, disymmetric, asymmetric
67what flower shape is the ancestral state?
asymmetric
68how are asymmetric flowers pollinated?
wind
69bees have a preference for
radial symmetry
70CYC specifies ______ and promotes ______
floral asymmetry, dorsal floral identity
71CYC expression is maintained by
class B and class C proteins
72CYC prevents _______ and restricts _________ in the dorsal domain
outgrowth of dorsal stamen, cell proliferation
73CYC is normally only in ________, but in backpetal flowers, CYC is also expressed in
Dorsal regions, lateral and ventral regions
74DIV specifies _______, but there must be ________
ventral floral identity, two copies
75without CYC, DICH, or DIV, the default state for petals is
lateral
76DIV is expressed _______, but only _______ confers ventral identity
throughout the flower, the bottom petal
77what do rad mutatnt flowers look like?
all petals ventral
78RAD acts as a ________ factor
anti-ventral
79what activates RAD expression?
CYC and DICH
80in the dorsal domain, RAD _______ DIV by _________
inhibits, stealing DRIF proteins
81in ventral domain, RAD ________, so DIV can ________
is absent, interact with DRIF
82sunflowers have two florets:
ray (yellow), and disc (dark)
83ray florets are _________ symmetric
bilaterally
84disc florets are _______ symmetric
radially
85CYC2c is only expressed in __________ in sunflowers
ray florets