1Process of cutting tissues into uniformly thin slices using a microtome?
Sectioning
2Instrument used to cut uniformly thin tissue sections?
Microtome
3Main purpose of sectioning?
To facilitate microscopic examination
4Three general types of tissue sections?
Paraffin, celloidin, and frozen sections
5Type of section prepared from paraffin-embedded tissue blocks?
Paraffin section
6Microtomes used for cutting paraffin sections?
Rocking and rotary microtomes
7Type of section prepared from celloidin-embedded tissues?
Celloidin section
8Microtome commonly used for cutting celloidin sections?
Sliding microtome
9Process of removing excess wax after embedding?
Trimming
10Minimum thickness of wax surrounding the tissue during trimming?
2 mm
11Process of trimming the sides, tips, and bottom of a tissue block?
Coarse trimming
12Tools used for coarse trimming?
Knife or blade
13Shape formed during coarse trimming?
Truncated pyramid or four-sided prism
14Process of exposing the entire tissue surface using a microtome?
Fine trimming
15Equipment used for fine trimming?
Microtome
16Adjuster setting indicated for fine trimming in the PPT?
15 mm
17Mechanism used to advance the tissue block during fine trimming?
Coarse feed mechanism
18Part of the tissue block exposed during fine trimming?
Entire tissue surface
19Finger used to pick up a cut tissue section?
Index finger
20Brush used to pick up cut tissue sections?
Camel hairbrush
21Flat tool used to pick up cut tissue sections?
Spatula
22Forceps used to pick up cut tissue sections?
Flat-bladed forceps
23Usual thickness of paraffin sections?
4–6 µm
24Usual thickness of celloidin sections?
10–15 µm
25Approximate thickness of frozen sections?
10 µm
26Usual thickness of semithin sections?
0.5–1 µm
27Usual thickness of ultrathin sections?
60–100 nm
28Fault where the clearing agent turns milky when tissue is placed in it?
Incomplete dehydration
29Reason water remains in tissue before clearing?
Incomplete dehydration
30Remedy when the clearing agent turns milky?
Repeat dehydration with absolute alcohol, then clear again
31Alcohol used to correct incomplete dehydration before clearing?
Absolute alcohol
32Fault where tissue is soft when the block is trimmed?
Incomplete fixation
33Remedy for soft tissue caused by incomplete fixation?
Repeat fixation
34Fault where frozen tissue crumbles during cutting?
Inadequate freezing
35Reason frozen tissue crumbles and comes off the block holder?
Inadequate freezing
36Remedy when frozen tissue crumbles during cutting?
Refreeze the tissue block
37Fault where airholes appear in tissue during trimming?
Incomplete impregnation
38Remedy when airholes appear during tissue trimming?
Repeat impregnation
39Fault where tissue shrinks away from wax during trimming?
Insufficient dehydration
40Processes affected by insufficient dehydration that cause tissue shrinkage?
Clearing and impregnation
41Remedy when tissue shrinks away from wax during trimming?
Repeat the whole procedure