Part 2

33 flashcardsby Daniela
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1
What fixative is good for preservation of glycogen and micro-incineration and is used to fix sputum?
Gendre's Fixative
2
What fluid is recommended for fixing mucopolysaccharides and nuclear proteins?
Newcomer's Fluid
3
What is the most common metallic fixative?
Mercuric Chloride
4
What is the routine fixative of choice for preservation of cell detail in tissue photography?
Mercuric Chloride
5
What fixative is recommended for renal tissue, fibrin, connective tissue, and muscle?
Mercuric Chloride
6
What solution permits brilliant staining of nuclear and connective tissue fibers?
Zenker's Solution
7
What solution is recommended for congested specimens such as lung, heart, and blood vessels and gives good results with PTAH and trichrome staining?
Zenker's Solution
8
What fixative is excellent for bone marrow, extramedullary hematopoiesis, and intercalated discs of cardiac muscle?
Zenker-Formol (Helly's) Solution
9
What fixative is an excellent microanatomic fixative for the pituitary gland, bone marrow, and blood-containing organs such as spleen and liver?
Zenker-Formol (Helly's) Solution
10
What fixative enhances nuclear detail for identifying normal and abnormal cell types in bone marrow?
Lillie's B-5 Fixative
11
What solution is recommended mainly for tumor biopsies, especially of the skin?
Heidenhain's Susa Solution
12
What fixative is traditionally used in electron microscopy as both a fixative and a heavy metal stain?
Osmium Tetroxide
13
What fixative is a good fixative and excellent stain for lipids in membranous structures and vesicles?
Osmium Tetroxide
14
What fixative fixes myelin and peripheral nerves well and preserves cytoplasmic structures such as Golgi bodies and mitochondria?
Osmium Tetroxide
15
What fixative is excellent for nuclear structures such as chromosomes?
Flemming's Solution
16
What fixative is recommended for cytoplasmic structures, particularly mitochondria, when acetic acid is omitted?
Flemming's Solution without Acetic Acid
17
What type of fixative uses chromic acid in a 1–2% aqueous solution and usually forms part of a compound fixative?
Chromate Fixatives
18
What type of fixative precipitates all proteins and adequately preserves carbohydrates?
Chromate Fixatives
19
What chemical preserves mitochondria when used at pH 4.5–5.2?
Potassium Dichromate
20
What fluid is recommended for demonstrating chromatin, mitochondria, mitotic figures, Golgi bodies, RBCs, and colloid-containing tissues?
Regaud's (Muller's) Fluid
21
What fluid is recommended for studying early degenerative processes and tissue necrosis?
Orth's Fluid
22
What fluid demonstrates Rickettsiae and other bacteria?
Orth's Fluid
23
What type of fixative is recommended for acid mucopolysaccharides and fixes connective tissue mucin?
Lead Fixatives
24
What type of fixative is excellent for glycogen demonstration, suitable for aniline stains, and highly explosive when dry?
Picric Fixatives
25
What solution is recommended for fixation of embryos and pituitary biopsies?
Bouin's Solution
26
What solution is sometimes recommended for gastro-intestinal tract biopsies, animal embryos, and endocrine gland tissue?
Bouin's Solution
27
What solution is recommended for gastro-intestinal tract specimens and endocrine tissues?
Hollande's Solution
28
What solution is highly recommended for preservation of glycogen and other carbohydrates?
Gendre's Solution
29
What acid is an essential constituent of most compound nuclear fixatives and solidifies at 17°C?
Glacial Acetic Acid
30
What acid is used for precipitation of proteins and nucleic acids and as a decalcifier and fixative in microscopy?
Trichloroacetic Acid (TCA)
31
What chemical is used to fix specimens at 0–4°C and is recommended for studying water-diffusible enzymes?
Acetone
32
What solution provides a stable medium for transporting fresh unfixed tissues for subsequent frozen section and immunofluorescence studies?
Michel's Solution
33
What solution is not suitable for transporting cells for flow cytometry or tissues used for fluorescent in-situ hybridization (FISH)?
Michel's Solution
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