CHAPTER 15

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1
Last step in tissue processing that produces a permanent histological preparation?
Mounting
2
Process performed after tissue sections are attached to slides and stained?
Mounting
3
Substance applied to slides to make tissue sections stick firmly?
Adhesive
4
Most commonly used adhesive for paraffin sections?
Mayer's egg albumin
5
Adhesive known for being easy to prepare, convenient, and inexpensive?
Mayer's egg albumin
6
Three components of Mayer's egg albumin?
Egg white, glycerin, and thymol crystals
7
Component of Mayer's egg albumin that increases viscosity?
Glycerin
8
Component of Mayer's egg albumin that prevents drying?
Glycerin
9
Component of Mayer's egg albumin that acts as a preservative?
Thymol crystals
10
Component of Mayer's egg albumin that prevents mold growth?
Thymol crystals
11
Adhesive stored in 70% alcohol until ready for staining?
Dried albumin
12
Alcohol concentration used to store dried albumin?
70% alcohol
13
Adhesive commonly prepared at a concentration of 1%?
Gelatin
14
Adhesive made by combining gelatin and formaldehyde?
Gelatin-formaldehyde mixture
15
Adhesive widely used in immunohistochemistry?
Poly-L-lysine
16
Adhesive particularly useful in cytology?
APES
17
Full name of APES?
3-Aminopropyltriethoxysilane
18
Adhesive useful for cytospin preparations of proteinaceous or bloody material?
APES
19
Syrupy fluid applied between the tissue section and coverslip after staining?
Mounting medium
20
Substance that holds the tissue section firmly beneath the coverslip?
Mounting medium
21
Substance that prevents movement of the coverslip?
Mounting medium
22
Mounting media components responsible for solidifying the medium?
Gelatin, glycerin jelly, and gum arabic
23
Mounting media component that prevents cracking of the preparation?
Glycerol
24
Mounting media component that prevents drying of the preparation?
Glycerol
25
Mounting media component that increases the refractive index?
Sugar
26
Type of mounting medium used for water-miscible preparations?
Aqueous mounting medium
27
Type of mounting medium suitable only for temporary mounting because of its low refractive index?
Water
28
Main disadvantage of water as a mounting medium?
Low refractive index
29
Aqueous mounting medium with a refractive index of 1.46?
Glycerin
30
Standard mounting medium for fat stains?
Glycerin
31
Aqueous mounting medium that may also serve as a preservative?
Glycerin
32
Refractive index of glycerin?
1.46
33
Aqueous mounting medium commonly used for immunofluorescence sections?
Phosphate-buffered glycerol
34
Refractive index of phosphate-buffered glycerol?
1.47
35
Standard mounting medium when dehydration and xylene clearing cannot be performed?
Glycerin jelly
36
Mounting medium commonly used for fat stains when dehydration and clearing are not possible?
Glycerin jelly
37
Refractive index of glycerin jelly?
1.47
38
Mounting medium recommended as an alternative to glycerin jelly?
Polyvinyl alcohol
39
Mounting medium often used in immunofluorescence microscopy?
Polyvinyl alcohol
40
Aqueous mounting medium that does not solidify during storage?
Farrant's medium
41
Refractive index of Farrant's medium?
1.43
42
Aqueous mounting medium used for methylene blue-stained nerve preparations?
Apathy's medium
43
Aqueous mounting medium used as a general-purpose mountant?
Apathy's medium
44
One of the most useful aqueous mounting media for fluorescent microscopy?
Apathy's medium
45
Refractive index of Apathy's medium?
1.52
46
Aqueous mounting medium recommended for frozen sections mounted directly from water?
Brun's fluid
47
Type of mounting medium used for preparations dehydrated and cleared in xylene or toluene?
Resinous mounting medium
48
Clearing agents used before resinous mounting media?
Xylene or toluene
49
Resinous mounting medium extracted from a Canadian tree?
Canada balsam
50
Scientific name of the Canadian tree that produces Canada balsam?
Abies balsamea
51
Resinous mounting medium recommended for whole mounts?
Canada balsam
52
Resinous mounting medium recommended for thick sections?
Canada balsam
53
Refractive index of Canada balsam?
1.524
54
Resinous mounting medium recommended for small tissue sections?
DPX
55
Resinous mounting medium unsuitable for whole mounts because of shrinkage during drying?
DPX
56
Reason DPX is not recommended for whole mounts?
Shrinkage during drying
57
Refractive index of DPX?
1.532
58
Resinous mounting medium with a refractive index of 1.52?
XAM
59
Refractive index of XAM?
1.52
60
Resinous mounting medium with a refractive index of 1.544?
Clarite
61
Refractive index of Clarite?
1.544
62
Resinous mounting medium manufactured by Fisher Scientific?
Permount
63
Company that manufactures Permount?
Fisher Scientific
64
Resinous mounting medium abbreviated as H.S.R.?
Harleco Synthetic Resin
65
Full name of H.S.R.?
Harleco Synthetic Resin
66
Resinous mounting medium associated with Gurr?
Clearmount
67
Manufacturer associated with Clearmount?
Gurr
68
Process of sealing the margins of a coverslip?
Ringing
69
Process that prevents fluid or semi-fluid mounting media from escaping?
Ringing
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