1A process in which a dehydrating agent is removed and replaced by a substance that dissolves wax?
clearing
2Recommended volume of clearing agent compared with tissue volume?
more than 10 times the tissue volume
3Most commonly used colorless clearing agent in histology laboratories?
xylene (xylol)
4Most rapid clearing agent, clearing tissue within 15–30 minutes?
xylene (xylol)
5What happens to xylene when incompletely dehydrated tissue is immersed in it?
it becomes milky
6Clearing agent with a clearing time of 1–2 hours that may substitute for xylene and benzene?
toluene
7Rapid-acting clearing agent with a clearing time of 15–60 minutes?
benzene
8Clearing agent that is highly flammable and carcinogenic?
benzene
9Clearing agent whose excessive exposure may damage the bone marrow and cause aplastic anemia?
benzene
10Clearing agent recommended for tough tissues such as skin, fibroid, and decalcified tissues?
chloroform
11Clearing agent recommended for nervous tissues, lymph nodes, and embryos because it causes minimum shrinkage and hardening?
chloroform
12Clearing agent suitable for large tissue specimens?
chloroform
13Clearing agent that is toxic to the liver after prolonged inhalation?
chloroform
14Clearing agent used to clear both paraffin and celloidin sections during the embedding process?
cedarwood oil
15Clearing agent recommended for central nervous system tissues and cytological studies, particularly smooth muscles and skin?
cedarwood oil
16Extremely slow clearing agent that takes 2–3 days?
cedarwood oil
17Clearing agent recommended for embryos, insects, and very delicate specimens because it can clear 70% alcohol without excessive tissue shrinkage and hardening?
aniline oil
18Clearing agent whose quality is not guaranteed because of its tendency to become adulterated?
clove oil
19Group of plant oils that were among the earliest transition solvents used in histology?
terpenes
20Examples of terpenes or plant oils used as transition solvents in histology?
turpentine, bergamot, cedarwood, clove, lemon, oreganum, and sandalwood oils
21Xylene substitute from the terpene family derived from citrus peel oil?
limonene