1Form of formaldehyde consisting of a 37% aqueous solution and gas?
Formalin
2Chemical used to sterilize HEPA filters in biological safety cabinets?
Formaldehyde
3Chemical whose disadvantages include irritability and carcinogenicity?
Formaldehyde
4Chemical disinfectant that is pseudomonacidal, tuberculocidal, fungicidal, and virucidal?
Glutaraldehyde
5Concentration of glutaraldehyde that is bactericidal in 10 minutes and sporicidal in 3–10 hours?
2%
6Halogens frequently used as disinfectants?
Iodine and chlorine
7Heavy metals used as disinfectants, including mercuric chloride and silver nitrate?
Heavy metals
8Substance historically used as an eye drop in newborns to prevent infection with N. gonorrhoeae?
1% silver nitrate
9Process by which halogens destroy microorganisms?
Oxidation
10Hypochlorite solution that should be freshly prepared daily and used with 10–30 minutes of contact time?
NaOCl
11Substance containing chlorine used to disinfect drinking water?
Halozone
12Iodine preparation containing 2% iodine in 70% alcohol and used as an antiseptic?
Tincture of iodine
13Preparation composed of iodine and a polymer that acts as an antiseptic or disinfectant?
Iodophor
14Recommended contact time for iodine on skin before blood collection?
30–60 seconds
15Halogen that is non-sporicidal and non-tuberculocidal?
Iodine
16Chlorine form commonly used for disinfection?
Hypochlorite
17CDC-recommended dilution of 5.25% NaOCl for tabletops and floors with blood spills?
1:10 dilution
18Chlorine contact time for organic materials?
3 minutes
19Chlorine contact time for mycobacteria?
10–30 minutes
20Major disadvantage of chlorine when large amounts of proteins are present?
Ineffective in the presence of large amounts of proteins
21Agents that destroy microorganisms by inactivating and precipitating cell proteins?
Heavy metals
22Examples of heavy metals used in microbial control?
Copper, arsenic, mercury, silver, and zinc
23Quaternary ammonium compounds characterized by low toxicity and used to disinfect laboratory surfaces?
QUATS
24Disinfectants commonly used on laboratory bench tops and other surfaces?
Quaternary Ammonium Compounds (QUATS)
25Disinfectants that are non-tuberculocidal and non-sporicidal and cause leakage of cell contents?
QUATS
26Example of a quaternary ammonium compound?
Zephiran
27Phenolic compounds that denature proteins and disrupt cell membranes?
Phenolics
28Expression of the bactericidal power of a substance compared with pure phenol?
Phenol coefficient
29Phenolic derivatives with reduced toxicity?
Phenolics
30Examples of phenolics?
Ortho-phenylphenol and ortho-benzyl-para-chlorophenol
31Examples of other phenolic disinfectants?
Chlorhexidine gluconate, chloroxylenol, and triclosan
32First widely used antiseptic and disinfectant that is tuberculocidal?
Phenol
33Phenol destroys the plasma membrane and denatures cell proteins?
Phenol
34Phenol contact time for mycobacteria using a 5% solution?
10–30 minutes
35Examples of phenol derivatives mentioned in the text?
Cresols, xylenols, amphyls, and hexachlorophenes
36Highest dilution of a disinfectant that kills bacteria after a 10-minute exposure?
Phenol coefficient
37Test organisms used for the phenol coefficient test?
Staphylococcus aureus and Salmonella typhi
38Gas sterilant used for plastic Petri dishes, sutures, catheters, and heat-sensitive equipment?
Ethylene oxide (EtO)
39Gas sterilant with explosive properties that should be mixed with nitrogen or carbon dioxide before use?
Ethylene oxide (EtO)
40Concentration of ethylene oxide used according to the text?
450–700 mg/L
41Temperature used for ethylene oxide sterilization according to the text?
55–60°C
42Biological indicator mentioned for ethylene oxide sterilization?
Bacillus species
43Chemical sterilant active against all vegetative microorganisms and fungal spores?
Peracetic acid
44Low-temperature exposure mentioned as important in food microbiology?
2–8°C for 72 hours
45Process that destroys bacteria through disruption of metabolism by removing water from microbes?
Desiccation
46Process that destroys bacteria through changes in proteins and decreased chemical reactions?
Lyophilization
47Bacterium that remains viable for about 1 hour in a dry environment?
Neisseria gonorrhoeae
48Mycobacterium that can remain viable for several months in a dry environment?
Mycobacterium tuberculosis
49Bacteria that can remain viable for up to ten years in a dry environment?
Bacillus and Clostridium
50Plan that identifies hazardous tasks and promotes safety?
Exposure control plan
51Safety measure involving instruction and orientation of employees?
Employee education and orientation
52Safety measure involving proper disposal of hazardous waste?
Appropriate disposal of hazardous waste
53Safety measure requiring blood and body fluids from every patient to be treated as potentially infectious?
Standard precautions
54Safety measure involving devices and procedures that reduce workplace hazards?
Engineering controls and safe work practices
55Protective equipment used to prevent exposure to infectious materials?
Personal protective equipment
56Plan followed after an occupational exposure?
Postexposure plan
57Precautions requiring blood and body fluids from every patient to be treated as potentially infectious?
Standard/Universal precautions
58Activity prohibited in the laboratory under standard precautions?
Eating, drinking, smoking, or applying cosmetics
59Practice prohibited because it can cause laboratory contamination or exposure?
Mouth pipetting
60People who should have access to the laboratory?
Trained personnel only
61Protective barriers used to prevent skin and mucous membrane exposure?
Gloves, goggles, and gowns
62Important hygiene practice under standard precautions?
Thorough handwashing
63Safety practice involving sharp objects?
Avoid injuries with sharp objects