1Process of making tissue components visible under the microscope using dyes or staining solutions?
Staining
2Purified coloring agent or crude dye usually applied in an aqueous solution?
Histologic stain
3Process of demonstrating tissue constituents and their relationships through interaction with dyes?
Histological staining
4Process that produces coloration of active tissue components?
Histological staining
5Staining method that directly colors tissue sections using aqueous or alcoholic dye solutions?
Direct staining
6Staining method that uses another agent to intensify the action of a dye?
Indirect staining
7Substance that acts as a bridge between tissue and dye to make staining possible?
Mordant
8Agent added to a dye to intensify the staining reaction?
Mordant
9Mordant used in Ehrlich's hematoxylin?
Potassium alum
10Mordant used in Weigert's hematoxylin?
Iron
11Agent that accelerates staining without directly participating in the reaction?
Accentuator
12Accentuator used in Loeffler's methylene blue?
Potassium hydroxide
13Accentuator used in carbol thionine?
Phenol
14Accentuator used in carbol fuchsin?
Phenol
15Staining method where tissue elements are stained in a definite sequence?
Progressive staining
16Staining method where solutions are applied for a specific time until sufficient color is obtained?
Progressive staining
17Staining method that does not require decolorization to achieve the desired color?
Progressive staining
18Staining method where tissues are first overstained before excess dye is removed?
Regressive staining
19Staining method where excess stain is removed until the desired color intensity is achieved?
Regressive staining
20Staining method that initially overstains tissue and may hide cellular details?
Regressive staining
21Selective removal of excess stain from tissue during regressive staining?
Differentiation
22Another term for differentiation in staining?
Decolorization
23Process that removes unwanted stain to reveal cellular details?
Differentiation
24Staining method that uses more than one chemical stain?
Differential staining
25Staining method used to distinguish microorganisms or cellular structures using different stains?
Differential staining
26Staining method where tissue develops a color different from the original dye?
Metachromatic staining
27Phenomenon where stained tissue shows a color different from the dye itself?
Metachromasia
28Staining method where tissue develops a color similar to the original dye?
Orthochromatic staining
29Staining method that produces color shades similar to the dye itself?
Orthochromatic staining
30Process that changes red-stained nuclei into blue-black after hematoxylin staining?
Blueing
31Process that converts the red color of alum hematoxylin-stained nuclei to blue-black?
Blueing
32Solution used to convert red hematoxylin-stained nuclei into blue-black?
Weak alkaline solution
33Color of nuclei immediately after staining with alum hematoxylin before blueing?
Red
34Color of nuclei after blueing?
Blue-black
35Common water-based reagent used for blueing?
Tap water
36Lithium-based reagent used for blueing?
Saturated lithium carbonate
37Ammonia concentration used as a blueing reagent?
0.5%
38Solvent used to prepare 0.5% ammonia for blueing?
Distilled water
39Artificial tap water solution used for blueing?
Scott's tap water substitute
40Process of demonstrating tissue elements using colorless metallic salt solutions?
Metallic impregnation
41Staining technique where metallic salts are reduced by tissues to form opaque deposits?
Metallic impregnation
42Usual color of the deposit formed during metallic impregnation?
Black
43Type of solutions used in metallic impregnation?
Colorless metallic salt solutions
44Process responsible for producing metallic deposits on tissue surfaces?
Reduction of metallic salts
45Staining method where stains are not directly taken up by the target structures?
Negative staining
46Staining method that reveals structures by outlining or filling the surrounding areas with stain?
Negative staining
47Staining method that makes structures visible by staining their background?
Negative staining
48Selective staining of living cell constituents?
Vital staining
49Staining method performed by injecting dye into an animal's body?
Intravital staining
50Type of vital staining performed inside a living animal?
Intravital staining
51Staining method used on cells immediately after removal from a living body?
Supravital staining
52Type of vital staining performed on freshly removed living cells?
Supravital staining
53Staining method that requires injection of dye into a living animal?
Intravital staining
54Staining method that does not require injection into the living body but uses freshly removed cells?
Supravital staining
55Additional stain applied in a different color to provide contrast?
Counterstain
56Stain used to provide background contrast to the primary stain?
Counterstain
57Common frozen-section staining method that uses hematoxylin and eosin?
Hematoxylin-Eosin method