CHAPTER 16

57 flashcardsby Daniela
Study these flashcardsFree · spaced repetition · iPhone, Android, Mac & web

All cards

1
Process of making tissue components visible under the microscope using dyes or staining solutions?
Staining
2
Purified coloring agent or crude dye usually applied in an aqueous solution?
Histologic stain
3
Process of demonstrating tissue constituents and their relationships through interaction with dyes?
Histological staining
4
Process that produces coloration of active tissue components?
Histological staining
5
Staining method that directly colors tissue sections using aqueous or alcoholic dye solutions?
Direct staining
6
Staining method that uses another agent to intensify the action of a dye?
Indirect staining
7
Substance that acts as a bridge between tissue and dye to make staining possible?
Mordant
8
Agent added to a dye to intensify the staining reaction?
Mordant
9
Mordant used in Ehrlich's hematoxylin?
Potassium alum
10
Mordant used in Weigert's hematoxylin?
Iron
11
Agent that accelerates staining without directly participating in the reaction?
Accentuator
12
Accentuator used in Loeffler's methylene blue?
Potassium hydroxide
13
Accentuator used in carbol thionine?
Phenol
14
Accentuator used in carbol fuchsin?
Phenol
15
Staining method where tissue elements are stained in a definite sequence?
Progressive staining
16
Staining method where solutions are applied for a specific time until sufficient color is obtained?
Progressive staining
17
Staining method that does not require decolorization to achieve the desired color?
Progressive staining
18
Staining method where tissues are first overstained before excess dye is removed?
Regressive staining
19
Staining method where excess stain is removed until the desired color intensity is achieved?
Regressive staining
20
Staining method that initially overstains tissue and may hide cellular details?
Regressive staining
21
Selective removal of excess stain from tissue during regressive staining?
Differentiation
22
Another term for differentiation in staining?
Decolorization
23
Process that removes unwanted stain to reveal cellular details?
Differentiation
24
Staining method that uses more than one chemical stain?
Differential staining
25
Staining method used to distinguish microorganisms or cellular structures using different stains?
Differential staining
26
Staining method where tissue develops a color different from the original dye?
Metachromatic staining
27
Phenomenon where stained tissue shows a color different from the dye itself?
Metachromasia
28
Staining method where tissue develops a color similar to the original dye?
Orthochromatic staining
29
Staining method that produces color shades similar to the dye itself?
Orthochromatic staining
30
Process that changes red-stained nuclei into blue-black after hematoxylin staining?
Blueing
31
Process that converts the red color of alum hematoxylin-stained nuclei to blue-black?
Blueing
32
Solution used to convert red hematoxylin-stained nuclei into blue-black?
Weak alkaline solution
33
Color of nuclei immediately after staining with alum hematoxylin before blueing?
Red
34
Color of nuclei after blueing?
Blue-black
35
Common water-based reagent used for blueing?
Tap water
36
Lithium-based reagent used for blueing?
Saturated lithium carbonate
37
Ammonia concentration used as a blueing reagent?
0.5%
38
Solvent used to prepare 0.5% ammonia for blueing?
Distilled water
39
Artificial tap water solution used for blueing?
Scott's tap water substitute
40
Process of demonstrating tissue elements using colorless metallic salt solutions?
Metallic impregnation
41
Staining technique where metallic salts are reduced by tissues to form opaque deposits?
Metallic impregnation
42
Usual color of the deposit formed during metallic impregnation?
Black
43
Type of solutions used in metallic impregnation?
Colorless metallic salt solutions
44
Process responsible for producing metallic deposits on tissue surfaces?
Reduction of metallic salts
45
Staining method where stains are not directly taken up by the target structures?
Negative staining
46
Staining method that reveals structures by outlining or filling the surrounding areas with stain?
Negative staining
47
Staining method that makes structures visible by staining their background?
Negative staining
48
Selective staining of living cell constituents?
Vital staining
49
Staining method performed by injecting dye into an animal's body?
Intravital staining
50
Type of vital staining performed inside a living animal?
Intravital staining
51
Staining method used on cells immediately after removal from a living body?
Supravital staining
52
Type of vital staining performed on freshly removed living cells?
Supravital staining
53
Staining method that requires injection of dye into a living animal?
Intravital staining
54
Staining method that does not require injection into the living body but uses freshly removed cells?
Supravital staining
55
Additional stain applied in a different color to provide contrast?
Counterstain
56
Stain used to provide background contrast to the primary stain?
Counterstain
57
Common frozen-section staining method that uses hematoxylin and eosin?
Hematoxylin-Eosin method
Study all 57 cards for free