1Process of trimming and cutting processed tissue into uniformly thin sections for microscopic study?
Microtomy
2Most common type of embedded tissue used in microtomy?
Paraffin-embedded tissue
3Process of removing excess wax to expose the tissue surface?
Trimming
4Process of cutting tissue into uniformly thin slices using a machine?
Sectioning
5Microtome part that holds the tissue in position?
Block holder or chuck
6Microtome parts responsible for cutting tissue sections?
Knife and knife carrier
7Microtome parts that position the tissue block and adjust section thickness?
Pawl, ratchet feed wheel, and adjustment screw
8Microtome also known as the Cambridge microtome?
Rocking microtome
9Inventor of the rocking microtome?
Paldwell Trefall
10Year the rocking microtome was invented?
1881
11Simplest type of microtome?
Rocking microtome
12Microtome used for serial sections of large paraffin-embedded tissue blocks?
Rocking microtome
13Inventor of the rotary microtome?
George Richards Minot
14Years the rotary microtome was invented?
1885–1886
15Most common microtome used in routine and research laboratories?
Rotary microtome
16Microtome commonly used for sectioning paraffin-embedded tissues?
Rotary microtome
17Developer of the sliding microtome?
George Adams
18Year the sliding microtome was developed?
1789
19Most dangerous type of microtome due to its movable exposed knife?
Sliding microtome
20Microtome used for cutting celloidin-embedded tissues?
Sliding microtome
21Type of sliding microtome used for all forms of embedding media?
Base sledge microtome
22Microtome especially suitable for hard tissues or large tissue blocks?
Base sledge microtome
23Microtome ideal for resin-embedded decalcified bone?
Base sledge microtome
24Type of microtome described as heavy-duty and electrically driven?
Base sledge microtome
25Part that moves in a base sledge microtome?
Block holder
26Part that remains stationary in a base sledge microtome?
Knife
27Type of sliding microtome developed for celloidin-embedded tissue blocks?
Standard sliding microtome
28Part that remains stationary in a standard sliding microtome?
Block holder
29Part that moves forward and backward in a standard sliding microtome?
Knife
30Type of sliding microtome considered more dangerous because of its moving knife?
Standard sliding microtome
31Inventor of the freezing microtome?
John Queckett
32Year the freezing microtome was invented?
1848
33Microtome used to cut undehydrated tissues in a frozen state?
Freezing microtome
34Microtome used for unembedded frozen sections?
Freezing microtome
35Microtome also called the cold microtome?
Cryostat
36Microtome most commonly used for rapid tissue preparation?
Cryostat
37Refrigerated apparatus used in fresh tissue microtomy?
Cryostat
38Device that maintains the temperature inside a cryostat?
Adjustable thermostat
39Temperature range maintained inside a cryostat?
-5°C to -30°C
40Average temperature maintained inside a cryostat?
-20°C
41Usual type of microtome found inside a cryostat?
Rotary microtome
42Microtome used to produce sections for electron microscopy?
Ultra-thin microtome
43Embedding medium used for ultra-thin microtomy?
Plastic resins
44Knives used in ultra-thin microtomy?
Glass and diamond knives
45Thickness of semi-thin sections?
0.5–1 micron
46Thickness of ultra-thin sections?
60–100 nanometers
47Conventional steel knife with one flat side and one concave side?
Plane concave knife
48Length of a plane concave knife?
25 mm
49Knife used in base sledge, rotary, and rocking microtomes?
Plane concave knife
50Side of a plane concave knife used for celloidin-embedded tissue?
Less concave side
51Side of a plane concave knife used for paraffin-embedded tissue?
More concave side
52Conventional steel knife with two concave sides?
Biconcave knife
53Length of a biconcave knife?
120 mm
54Microtome that uses a biconcave knife?
Rotary microtome
55Type of tissue section cut using a biconcave knife?
Paraffin-embedded section
56Conventional steel knife with two straight sides?
Plane wedge knife
57Length of a plane wedge knife?
100 mm
58Knife used in base sledge or sliding microtomes?
Plane wedge knife
59Knife used for frozen sections or very hard and tough paraffin-embedded tissues?
Plane wedge knife
60Angle formed between the actual cutting edges of a microtome knife?
Bevel angle
61Normal bevel angle of a microtome knife?
27°–32°
62Angle that provides optimum tissue penetration and minimizes distortion?
Inclination angle
63Optimum inclination angle of a microtome knife?
15°
64Angle formed between the tissue block surface and the knife's cutting edge?
Clearance angle
65Range of the clearance angle?
0°–15°
66Recommended clearance angle to prevent uneven sections?
5°–10°
67Knife angle adjustment that prevents alternating thin and thick sections?
Proper clearance angle
68Process of removing irregularities and nicks from conventional steel knives?
Honing
69Another term for honing?
Hard sharpening
70Direction of knife movement during honing?
Heel to toe
71Knife edge position during honing?
Edge f