1describe the appearance of a spore within a GPR
- non staining oval body inside of a rod
- dark blue bud on the end of an organism
- clear unstained halo around the organism
- unevenly shaped pink body within the rod
2which 2 genuses are associated with the development of rods
- Bacillus spp.
- Clostridium/Clostridiodes spp.
- Lactobacillus spp.
- Corynebacterium spp.
3what is the significance of performing a culture test when working with Bacillus spp
- rule out B. anthracis
- rule out B. cereus
- determine oxygen requirements
- determine which % of organisms have spores
4which dye can be used to enhance spore visualization
- malachite green
- safranin
- methylene blue
- india ink
5which dye is used to visualize capsules in Bacillus spp.
- india ink
- safranin
- methylene blue
- malachite green
6which test result/chracteristic can differentiate Bacillus spp. from Clostridium spp.
- positive catalase
- negative catalase
- gamma hemolytic
- spore presence
7which organism fits the following?
- non motile
- no PEA growth
- Lecithinase positive
- no MSA growth/fermentation
- larger ZOI with penicillin
- Bacillus anthracis
- Bacillus cereus
- Bacillus coagulans
- Bacillus subtilis
8which organism results in the formation of clinically significant, encapsulated chains of 2-4 cells in blood or CSF
- Bacillus anthracis
- Bacillus cereus
- Bacillus subtilis
- Bacillus coagulans
9in which conditions will spores form?
- ambient air
- high CO2 conditions
- >15 psi
- on egg yolk agar (EYA) only
10which organism is non hemolytic, shows no growth on CNA/MAC/PEA, and can resemble a medusa head?
- B. anthracis
- B. cereus
- B. pertussis
- B. henslae
11which disease states can result form B. anthracis infection (choose all that apply)
- cutaneous anthrax
- inhalation anthrax
- GI anthrax
- oropharyngeal anthrax
- orbital anthrax
- genital anthrax
12what is the black, necrotic lesion associated with anthrax called?
13which two factors, when produced together, are fatal in B. anthracis infections
- lethal toxin, edema toxin
- lethal toxin, hemolysin
- edema toxin, emetic toxin
- protein capsule, phospholipases
14T/F - the presence of just one toxin produced by B. anthracis can be fatal
15which species is most associated with sporadic food poisoning outbreaks?
- Bacillus cereus
- Bacillus anthracis
- Corynebacterium jeikeium
- Listeria monocytogenes
16which of the following are microbial defense mechanisms of B. cereus?
- all of the above
- spore formation
- phospholipase aids
- hemolysins
- beta lactamase, protease, collagenase
- none of the above
17which syndromes/menifestations are associated with B. cereus? (choose all that apply)
- diarrheal
- emetic
- necrotic lesions
- purulent red rash
18T/F - Bacillus spp. are generally beta hemolytic, but B. anthracis is not
19the following is associated with which genus?
- diphtheroids
- may form stacking patterns like a fence microscopically
- resembles Chinese lettering
- often seen with PMNs
- Corynebacterium spp.
- Capnocytophaga spp.
- Campylobacter spp.
- Cardiobacterium spp.
20which genus fits the following profile
- catalase positive
- motility negative
- nitrate positive
- urea positive
- esculin negative
- gelatin liquefaction
- Corynebacterium
- Lactobacillus
- Erysipelothrix
- Brucella
21T/F - only toxigenic strains of Corynebacterium diphtheriae cause disease
22how can you microscopocally ID C. diphtheriae
- metachromatic granules on growth from Loeffler's medium stained with methylene blue, beaded areas within the cell
- long string of beads, GNR
- flowery arrangements of GPC
- seagull-shaped GPC
23which of the following is NOT a type of media that can be used to grow C. diphtheriae
- cyanide tellurite
- Tinsdale
- cystine tellurite
- potassium tellurite
24describe the appearance of C. diphtheriae on Tinsdale media
- graylack with brown halos
- plain black
- dark gray
- metachromatic
25why is Loeffler's medium useful for C. diphtheriae
- encourages growth of metachromatic granules
- turns colonies black
- colonies turn pink due to tellurite usage
- contains yeast to boost growth
26what gene within the infective organism is responsible for pharyngeal diphtheriae
- tox gene
- bla gene
- tet gene
- vanB gene
27which organism can cause gray/white pseudomembranes to develop as a result of toxin secretion from epithelial cells
- C. diphtheriae
- S. pyogenes
- S. agalactiae
- C. urealyticum
28which organism can release toxins that cause damage to the kidneys, heart, and nervous system, and eventually lead to paralysis
- C. diptheriae
- C. urealyticum
- C. jeikeium
- C. resistens
29which Corynebacterium strains can cause pharyngeal diphtheria
- all of the above
- C. diphtheriae
- C. ulcerans
- C. pseudotuberculosis
30which Corynebacterium species are associated with unpasteurized dairy or contact with animals
- C. ulcerans
- C. pseudotuberculosis
- C. jeikeium
- C. diphtheriae
31what causes C. jeikeium colonies to appear pin-point upon isolation
- lipid requirements
- carb requirements
- amino acid requirements
- presence of factor X
32how can you rule out C. resistens
- oxidation of glucose, positive pyrazinamidase, no anerobic growth
- optochin and bacitracin resistance
- novobiocin resistance, oxidation of lactose, black colonies on tellurite
- beta hemolysis on SBA, no anaerobic growth
33which is the most common diphtheroid isolate
- C. jeikeium
- C. diphtheriae
- C. resistens
- C. urealyticum
34which groups are at the highest risk for contracting C. jeikeium
- all of the above
- immunocompromised people
- malignancies
- those undergoing invasive procedures
35which crystals are associated with a C. urealyticum infection
- struvite
- cysteine
- ammonia
- cholesterol
36how can you increase the chances of isolating C. urealyticum from a urine sample
- hold for >24hrs
- culture immediately
- use a clean catch specimen
- use a sample gathered via suprepubic aspiration
37which organism exhibits tumbling motility and yields an umbrella shape in solid agar at room temp
- Listeria monocytogenes
- Corynebacterium resistens
- Arcanobacerium spp.
- Bacillus anthracis
38at what temp does Listeria monocytogenes yield an umbrella shape in solid agar
39which organism may resemble group B strep and react with group B/G antisera
- Listeria monocytogenes
- Cornybacterium urealyticus
- Cornybacterium diphtheriae
- Erysipelothrix rhusiopathiae
40describe the key biochem reactions of L. monocytogenes
- catalase pos, esculin pos, moltility pos, CAMP pos
- oxidase negative, catalase pos, esculin negative, PYR pos
- catalase neg, esculin neg, moltility neg, CAMP pos
- catalase pos, esculin neg, moltility neg, CAMP pos
41what is the most common method of contracting Listeria monocytogenes
- contaminated food consumption
- unpasteurized dairy
- laboratory acquired
- prolonged hospital stay
42Listeria monocytogenes is:
- intracellular
- extracellular
43which organism yields flu-like symptoms in pregnant women, cross the placenta once she becomes septic, and result in premature birth, stillbirth, or spontaneous abortion
- Listeria monocytogenes
- Arcanobacterium spp.
- Corynebacterium spp.
- Bacillus anthracis
44how can you differentiate Arcanobacterium pyogenes/haemolyticum from Arcanobacterium bernardiae on a plate
- A. pyogenes/haemolyticum are beta hemolytic, A. bernardiae is gamma hemolytic
- A. pyogenes/haemolyticum are gamma hemolytic, A. bernardiae is beta hemolytic
- A. pyogenes/haemolyticum are alpha hemolytic, A. bernardiae is gamma hemolytic
- A. pyogenes/haemolyticum are beta hemolytic, A. bernardiae is alpha hemolytic
45how can you differentiate A. haemolyticum from A. pyogenesernardiae
- A. haemolyticum is CAMP inhibition positive/xylose and gelatin negative, A. pyogenesernardiae is the opposite
- A. haemolyticum is CAMP inhibition negative/xylose and gelatin negative, A. pyogenesernardiae is the opposite
- A. haemolyticum is CAMP inhibition positive/xylose and gelatin positive, A. pyogenesernardiae is the opposite
- A. haemolyticum is CAMP inhibition negative/xylose and gelatin positive, A. pyogenesernardiae is the opposite
46what is the clinical significance of Arcanobacterium haemolyticum
- pharyngitis in young people, rare systemic infections
- found in animals, infections occurring in rural settings
- isolates from immunocompromised individuals
- acquired from unpasteurized dairy
47what is the clinical significance of Arcanobacterium pyogenes
- found in animals, infections occurring in rural settings
- pharyngitis in young people, rare systemic infections
- isolates from immunocompromised individuals
- acquired from unpasteurized dairy
48what is the clinical significance of Arcanobacterium bernardiae
- isolates from immunocompromised individuals
- acquired from unpasteurized dairy
- pharyngitis in young people, rare systemic infections
- found in animals, infections occurring in rural settings
49what is the main reservoir of Erysipelothrix rhusiopathiae
- domestic swine
- wild swine
- birds
- arthropods
50which organism is H2S positive along the stab line only
- Erysipelothrix rhusiopathiae
- Listeria monocytogenes
- Corynebacterium diphtheriae
- Arcanobacterium bernardiae
51which organism is associated with the formation of an erysipeloid (diamond shaped rash on the hands or fingers 2-7 days after exposure)
- Erysipelothrix rhusiopathiae
- Listeria monocytogenes
- Corynebacterium diphtheriae
- Arcanobacterium bernardiae
52best growth media for Gardneralla vaginalis
- human blood bilayer tween (HBT)
- sheep blood agar (SBA)
- cetrimide
- BCSA
53which organism fits the following
- starch hydrolysis pos
- hippurate hydrolysis pos
- beta glucosidase neg
- alpha glucosidase pos
- Gardnerella vaginalis
- C. urealyticum
- L. monocytogenes
- A. haemolyticum