exam 4

54 flashcardsby Montana
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1
describe the appearance of a spore within a GPR
  • non staining oval body inside of a rod
  • dark blue bud on the end of an organism
  • clear unstained halo around the organism
  • unevenly shaped pink body within the rod
2
which 2 genuses are associated with the development of rods
  • Bacillus spp.
  • Clostridium/Clostridiodes spp.
  • Lactobacillus spp.
  • Corynebacterium spp.
3
what is the significance of performing a culture test when working with Bacillus spp
  • rule out B. anthracis
  • rule out B. cereus
  • determine oxygen requirements
  • determine which % of organisms have spores
4
which dye can be used to enhance spore visualization
  • malachite green
  • safranin
  • methylene blue
  • india ink
5
which dye is used to visualize capsules in Bacillus spp.
  • india ink
  • safranin
  • methylene blue
  • malachite green
6
which test result/chracteristic can differentiate Bacillus spp. from Clostridium spp.
  • positive catalase
  • negative catalase
  • gamma hemolytic
  • spore presence
7
which organism fits the following?
- non motile
- no PEA growth
- Lecithinase positive
- no MSA growth/fermentation
- larger ZOI with penicillin
  • Bacillus anthracis
  • Bacillus cereus
  • Bacillus coagulans
  • Bacillus subtilis
8
which organism results in the formation of clinically significant, encapsulated chains of 2-4 cells in blood or CSF
  • Bacillus anthracis
  • Bacillus cereus
  • Bacillus subtilis
  • Bacillus coagulans
9
in which conditions will spores form?
  • ambient air
  • high CO2 conditions
  • >15 psi
  • on egg yolk agar (EYA) only
10
which organism is non hemolytic, shows no growth on CNA/MAC/PEA, and can resemble a medusa head?
  • B. anthracis
  • B. cereus
  • B. pertussis
  • B. henslae
11
which disease states can result form B. anthracis infection (choose all that apply)
  • cutaneous anthrax
  • inhalation anthrax
  • GI anthrax
  • oropharyngeal anthrax
  • orbital anthrax
  • genital anthrax
12
what is the black, necrotic lesion associated with anthrax called?
  • eschar
  • buboe
  • macule
  • escule
13
which two factors, when produced together, are fatal in B. anthracis infections
  • lethal toxin, edema toxin
  • lethal toxin, hemolysin
  • edema toxin, emetic toxin
  • protein capsule, phospholipases
14
T/F - the presence of just one toxin produced by B. anthracis can be fatal
  • false
  • true
15
which species is most associated with sporadic food poisoning outbreaks?
  • Bacillus cereus
  • Bacillus anthracis
  • Corynebacterium jeikeium
  • Listeria monocytogenes
16
which of the following are microbial defense mechanisms of B. cereus?
  • all of the above
  • spore formation
  • phospholipase aids
  • hemolysins
  • beta lactamase, protease, collagenase
  • none of the above
17
which syndromes/menifestations are associated with B. cereus? (choose all that apply)
  • diarrheal
  • emetic
  • necrotic lesions
  • purulent red rash
18
T/F - Bacillus spp. are generally beta hemolytic, but B. anthracis is not
  • true
  • false
19
the following is associated with which genus?
- diphtheroids
- may form stacking patterns like a fence microscopically
- resembles Chinese lettering
- often seen with PMNs
  • Corynebacterium spp.
  • Capnocytophaga spp.
  • Campylobacter spp.
  • Cardiobacterium spp.
20
which genus fits the following profile
- catalase positive
- motility negative
- nitrate positive
- urea positive
- esculin negative
- gelatin liquefaction
  • Corynebacterium
  • Lactobacillus
  • Erysipelothrix
  • Brucella
21
T/F - only toxigenic strains of Corynebacterium diphtheriae cause disease
  • true
  • false
22
how can you microscopocally ID C. diphtheriae
  • metachromatic granules on growth from Loeffler's medium stained with methylene blue, beaded areas within the cell
  • long string of beads, GNR
  • flowery arrangements of GPC
  • seagull-shaped GPC
23
which of the following is NOT a type of media that can be used to grow C. diphtheriae
  • cyanide tellurite
  • Tinsdale
  • cystine tellurite
  • potassium tellurite
24
describe the appearance of C. diphtheriae on Tinsdale media
  • graylack with brown halos
  • plain black
  • dark gray
  • metachromatic
25
why is Loeffler's medium useful for C. diphtheriae
  • encourages growth of metachromatic granules
  • turns colonies black
  • colonies turn pink due to tellurite usage
  • contains yeast to boost growth
26
what gene within the infective organism is responsible for pharyngeal diphtheriae
  • tox gene
  • bla gene
  • tet gene
  • vanB gene
27
which organism can cause gray/white pseudomembranes to develop as a result of toxin secretion from epithelial cells
  • C. diphtheriae
  • S. pyogenes
  • S. agalactiae
  • C. urealyticum
28
which organism can release toxins that cause damage to the kidneys, heart, and nervous system, and eventually lead to paralysis
  • C. diptheriae
  • C. urealyticum
  • C. jeikeium
  • C. resistens
29
which Corynebacterium strains can cause pharyngeal diphtheria
  • all of the above
  • C. diphtheriae
  • C. ulcerans
  • C. pseudotuberculosis
30
which Corynebacterium species are associated with unpasteurized dairy or contact with animals
  • C. ulcerans
  • C. pseudotuberculosis
  • C. jeikeium
  • C. diphtheriae
31
what causes C. jeikeium colonies to appear pin-point upon isolation
  • lipid requirements
  • carb requirements
  • amino acid requirements
  • presence of factor X
32
how can you rule out C. resistens
  • oxidation of glucose, positive pyrazinamidase, no anerobic growth
  • optochin and bacitracin resistance
  • novobiocin resistance, oxidation of lactose, black colonies on tellurite
  • beta hemolysis on SBA, no anaerobic growth
33
which is the most common diphtheroid isolate
  • C. jeikeium
  • C. diphtheriae
  • C. resistens
  • C. urealyticum
34
which groups are at the highest risk for contracting C. jeikeium
  • all of the above
  • immunocompromised people
  • malignancies
  • those undergoing invasive procedures
35
which crystals are associated with a C. urealyticum infection
  • struvite
  • cysteine
  • ammonia
  • cholesterol
36
how can you increase the chances of isolating C. urealyticum from a urine sample
  • hold for >24hrs
  • culture immediately
  • use a clean catch specimen
  • use a sample gathered via suprepubic aspiration
37
which organism exhibits tumbling motility and yields an umbrella shape in solid agar at room temp
  • Listeria monocytogenes
  • Corynebacterium resistens
  • Arcanobacerium spp.
  • Bacillus anthracis
38
at what temp does Listeria monocytogenes yield an umbrella shape in solid agar
  • room temp
  • 4C
  • 37C
  • body temp
39
which organism may resemble group B strep and react with group B/G antisera
  • Listeria monocytogenes
  • Cornybacterium urealyticus
  • Cornybacterium diphtheriae
  • Erysipelothrix rhusiopathiae
40
describe the key biochem reactions of L. monocytogenes
  • catalase pos, esculin pos, moltility pos, CAMP pos
  • oxidase negative, catalase pos, esculin negative, PYR pos
  • catalase neg, esculin neg, moltility neg, CAMP pos
  • catalase pos, esculin neg, moltility neg, CAMP pos
41
what is the most common method of contracting Listeria monocytogenes
  • contaminated food consumption
  • unpasteurized dairy
  • laboratory acquired
  • prolonged hospital stay
42
Listeria monocytogenes is:
  • intracellular
  • extracellular
43
which organism yields flu-like symptoms in pregnant women, cross the placenta once she becomes septic, and result in premature birth, stillbirth, or spontaneous abortion
  • Listeria monocytogenes
  • Arcanobacterium spp.
  • Corynebacterium spp.
  • Bacillus anthracis
44
how can you differentiate Arcanobacterium pyogenes/haemolyticum from Arcanobacterium bernardiae on a plate
  • A. pyogenes/haemolyticum are beta hemolytic, A. bernardiae is gamma hemolytic
  • A. pyogenes/haemolyticum are gamma hemolytic, A. bernardiae is beta hemolytic
  • A. pyogenes/haemolyticum are alpha hemolytic, A. bernardiae is gamma hemolytic
  • A. pyogenes/haemolyticum are beta hemolytic, A. bernardiae is alpha hemolytic
45
how can you differentiate A. haemolyticum from A. pyogenesernardiae
  • A. haemolyticum is CAMP inhibition positive/xylose and gelatin negative, A. pyogenesernardiae is the opposite
  • A. haemolyticum is CAMP inhibition negative/xylose and gelatin negative, A. pyogenesernardiae is the opposite
  • A. haemolyticum is CAMP inhibition positive/xylose and gelatin positive, A. pyogenesernardiae is the opposite
  • A. haemolyticum is CAMP inhibition negative/xylose and gelatin positive, A. pyogenesernardiae is the opposite
46
what is the clinical significance of Arcanobacterium haemolyticum
  • pharyngitis in young people, rare systemic infections
  • found in animals, infections occurring in rural settings
  • isolates from immunocompromised individuals
  • acquired from unpasteurized dairy
47
what is the clinical significance of Arcanobacterium pyogenes
  • found in animals, infections occurring in rural settings
  • pharyngitis in young people, rare systemic infections
  • isolates from immunocompromised individuals
  • acquired from unpasteurized dairy
48
what is the clinical significance of Arcanobacterium bernardiae
  • isolates from immunocompromised individuals
  • acquired from unpasteurized dairy
  • pharyngitis in young people, rare systemic infections
  • found in animals, infections occurring in rural settings
49
what is the main reservoir of Erysipelothrix rhusiopathiae
  • domestic swine
  • wild swine
  • birds
  • arthropods
50
which organism is H2S positive along the stab line only
  • Erysipelothrix rhusiopathiae
  • Listeria monocytogenes
  • Corynebacterium diphtheriae
  • Arcanobacterium bernardiae
51
which organism is associated with the formation of an erysipeloid (diamond shaped rash on the hands or fingers 2-7 days after exposure)
  • Erysipelothrix rhusiopathiae
  • Listeria monocytogenes
  • Corynebacterium diphtheriae
  • Arcanobacterium bernardiae
52
best growth media for Gardneralla vaginalis
  • human blood bilayer tween (HBT)
  • sheep blood agar (SBA)
  • cetrimide
  • BCSA
53
which organism fits the following
- starch hydrolysis pos
- hippurate hydrolysis pos
- beta glucosidase neg
- alpha glucosidase pos
  • Gardnerella vaginalis
  • C. urealyticum
  • L. monocytogenes
  • A. haemolyticum
54
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