1which is NOT a characteristic of non-fermenting bacilli
- sweet, fruity odor
- not typically found in healthy human flora
- can be acquired in the environment or hospital settings
- prefer dry environments
2what is the clinical significance of Haemophilus ducreyi?
- associated with subacute endocarditis
- infections in CF patients can be life threatening
- acquired when swimming in warm water, may infect open wounds
- STI causing painful chancres
3which media allows for the enhancement of the pigment pyocyanin
- cetrimide (Pseudocel)
- BSCA
- none of the above
- MAC
4which organism fits the following:
- produces alginate slime
- beta hemolytic
- none lactose fermenting
- oxidase positive
- grape-like odor
- Helicobacter pylori
- Pseudomonas aeruginosa
- Bordetella pertussis
- Escherichia coli
5why is pyocyanin significant
- masks P. aeruginosa from the immune system
- prevents upper respiratory cilia from removing B. cepacia
- prevents upper respiratory cilia from removing P. aeruginosa
- creates a distinctive color for identifying B. cepacia
6which organism is associated with colonization in CF patients, chronic osteomyelitis, swimmer's ear, bacterial keratitis, and a wide range of other infectious diseases?
- P. aeruginosa
- B. quintana
- F. tularensis
- B. cepacia
7T/F - P. aeruginosa is now showing intrinsic resistance to many antibiotics
8which is NOT a key difference between P. aeruginosa and P. fluorescens/P. putida?
- P. fluorescens is oxidase positive, P. aeruginosa is oxidase negative
- P. aeruginosa grows at 42C, P. fluorescens/putida do not
- P. aeruginosa can reduce nitrate, P. fluorescens/putida do not
- P. fluorescens/putida produces pyoverdin and fluorescein and not pyocyanin, while P. aeruginosa produces all three
9which Pseudomonas species is known for being nonfluorescent and having a wrinkly, brown appearance on SBA?
- P. fluorescens
- P. putida
- P. aeruginosa
- P. stutzeri
10which organism fits the following?
- motile GNR
- associated with CF patients
- not part of normal flora
- dirt-like odor
- P. aeruginosa
- B. cepacia complex
- Acinetobacter spp.
- Nocardia spp.
11describe the appearance of B. cepacia complex on BCSA
- changes surrounding media from red to yellow
- changes surrounding media from yellow to clear
- yields wide feathery colonies
- yields small, pinpoint colonies
12why is B. cepacia complex so dangerous for CF patients?
- its ability to adhere to mucin, which is overproduced by CF patients
- it grows at the base of lower respiratory milia, making it hard to remove
- it encourages mucin production when infecting a CF patient
- it possesses many virulence factors that the body can't defend against
13which organism fits the following description:
- aerobic GNR
- oxidase positive
- musty/earthy odor
- arginine positive
- caustive agent of meliodosis
- Pseudomonas stutzeri
- Stenotrophomonas maltophilia
- Burkholderia pseudomallei
- Burkholderia cepacia
14where is Elizabethkingia meningoseptica found/acquired?
- environmental/hospital sources
- normal human flora
- environmental sources only
- animal reservoirs (arthropods, rodents)
15which organism fits the following:
- positive indole with erlich's reagent
- produces beta lactamases
- oxidase positive
- non motile
- Escherichia coli
- Alcaligenes faecalis
- Klebsiella pneumoniae
- Elizabethkingia meningoseptica
16which organism fits the following
- green apple odor
- green discoloration
- motile GNR
- assachrolytic
- not part of the human flora, but may colonize CF patients
- Alcaligenes faecalis
- Aeromonas spp.
- Elizabethkingia meningoseptica
- Achromobacter xylosoxidans
17which two sugars can be oxidzed by Achromobacter xylosoxidans?
- glucose, sucrose
- sorbitol, innositol
- glucose, xylose
- lactose, xylose
18T/F - Vibrio spp. are not part of human flora and should be considered pathogenic when found
true
19which organism fits the following:
- found in salt/freshwater
- associated with undercooked seafood and poor sanitation
- comma shaped GNR
- shooting star motility in fresh stool
- Vibrio vulnificus
- Vibrio cholerae
- Vibrio parahaemolyticus
- Eswardsiella tardia
20which is a defining characteristic of Vibrio cholerae
- will grow in 6% salt
- blood group O individuals are more susceptible
- all of the above
- yields a 7mm or taller height during string test
21which of the following is correct
- V. cholerae TSI: A/A, V. parahaemolyticus TSI: K/A
- both V. cholerae and V. parahaemolyticus are A/A
- V. cholerae TSI: K/A, V. parahaemolyticus TSI: A/A
- both V. cholerae and V. parahaemolyticus are K/A
22how can you differ V. cholerae and V. parahaemolyticus on TCBS agar?
- V. cholerae is clear, V. parahaemolyticus is red
- V. cholerae is blue green, V. parahaemolyticus is yellow
- V. cholerae is yellow, V. parahaemolyticus is blue green
- there is no difference
23what organism is associated with the presence of "rice water stool"
- V. parahaemolyticus
- V. cholerae
- Y. entercolitica
- E. coli
24which virulence factor(s) are found in V. parahaemolyticus
- hemolysin
- enterotoxin
- cytotoxin
- all of the above
25which allows for the development of septicemia as a result from V. vulnificus?
- high iron levels in serum
- high calcium levels in serum
- high magnesium levels in serum
- high sodium levels in serum
26which organism fits the following
- lactose fermenter on MAC
- indole positive
- halophilic, grows in conditions up to 1% NaCl
- 3-6mm in string test
- sensitive to 10/150 micrograms of O/129
- ferments salicin
- A. hydrophila
- V. vulnificus
- V. parahaemolyticus
- V. cholerae
27_____ fermentation will distinguish V. vulnificus from other Vibrio spp.
- salicin
- sodium deoxycholate
- iron
- hemolysin
28what compound is considered vibriostatic
- ciprofloxacin
- O/129
- none of the above
- O157:H7
29what organism is positive for oxidase, motility, lysine decarboxylase, indole, DNase, and gelatinase
- Aeromonas
- none of the above
- all of the above
- Vibrio spp.
30which is a valid test for differentiating Vibrio spp. from Aeromonas
- ornithine decarboxylase
- indole
- motility
- oxidase
31swimming in warm water with an open wound can result in ______ exposure, potentially causing skin infections and septicemia
- Burkholderia cepacia
- Vibrio cholerae
- Pseudomonas aeruginosa
- Aeromonas hydrophila
32what organism is thought to smell like almonds, cause wound infections, and produce a pigment called violacein?
- Pseudomonas fluorescens
- Alcaligenes spp.
- Vibrio parahaemolyticus
- Chromobacterium violaceum
33what does a positive ALA (aminolevulinic acid)/porphyrin test indicate?
- ability to produce factor X and V
- ability to produce factor X
- proves sensitivity to ALA
- ability to produce factor V
34what is the importance of HBA (horse blood agar)
- allows for visualization of hemolysis caused by Haemophilus spp.
- provides factor V to grow H. parainfluenzae
- none of the above
- demonstrates satellitism when streaked with S. aureus and any Haemophilus spp.
35T/F - the HACEK group contains these organisms:
- Aggregatibacter aphrophilus (formerly Haemophilus aphrophilus)
- Aggregatibacter actinomycetocomitans
- Cardiobacterium hominis
- Edwardsiella tarda
- Kingella kingae
36what is the clinical significance of the HACEK group
- they're associated with chronic bacterial endocarditis
- they cause about 3% of cases of subacute bacterial endocarditis
- the group is resistant to most weidely used antibiotics
- they're some of the most infectious bacterial agents in America
37which organism fits the following:
- smells like liquid glue
- requires factor X for isolation but neither X or V for growth
- catalase/indolerease negative
- glucose and lactose positive
- Aggregatibacter actinomycetocomitans
- Kingella kingae
- Aggregatibacter aphrophilus
- Cardiobacterium hominis
38which organism is most often associated with localized juvenile periodontitis
- Aggregatibacter aphrophilus
- Haemophilus ducreyi
- Cardiobacterium hominis
- Aggregatibacter actinomycetocomitans
39which member of the HACEK group fits the following:
- pleomorphic, flowery arrangement on gram stains
- alpha hemolytic
- oxidase, nitrate, and indole positive
- catalase negative
- glucose, maltose, sucrose positive
- Aggregatibacter aphrophilus
- Cardiobacterium hominis
- Aggregatibacter actinomycetocomitans
- Kingella kingae
40which member of the HACEK group fits the following:
- bleach odor
- no carbohydrate fermentation
- oxidase/nitrate positive
- associated with human bites/fist fights - leading to infections in IV drug users who lubricate their needles with saliva
- Eikenella corrodens
- Aggregatibacter aphrophilus
- Aggregatibacter actinomycetocomitans
- Kingella kingae
41which genus contains 17 species that are normal flora in the GI/nasopharyngeal tracts of dogs, cats, and other wild animals?
- Pasteurella
- Haemophilius
- Capnocytophaga
- Echerichia
42a child presents to the ER with an infected cat bite that is releasing purulent matieral and has become rapidly inflamed over the past day. a swab was taken and yielded the following:
- catalase/oxidase positive
- glucose fermenting
- weak acid production in TSI
which organism is the most likely culprit?
- Pasteurella multocida
- Capnocytophaga ochracea
- Vibrio parahaemolyticus
- Capnocytophaga canimorsus
43how can you differentiate the Capnocytophaga species that infect humans vs cats/dogs?
- human species are oxidase positive/catalase negative, cat/dog species are the opposite
- human species are oxidase negative/catalase positive, cat/dog species are the opposite
- human species are oxidase/catalase positive, cat/dog species are oxidase/catalase negative
- human species are oxidase/catalase negative, cat/dog species are oxidase/catalase positive
44what makes Capnocytophaga a dangerous pathogen?
- human species are extremely contagious
- produces immunosuppressive factors
- species are resistant to many forms of treatment
- few organisms are required to cause an infection
45what do H. influenzae and H. aegyptius have in common?
- will satellite around NAD producing colonies like S. aureus
- ALA negative
- all of the above
- require X and V factor
46which organism is responsible for Brazilian purpuric fever
- A. aphrophilus
- H. parainfluenzae
- H. aegyptius
- H. influenzae
47which Haemophilus species fits the following:
- ALA positive
- requires factor V for growth
- gamma hemolysis on HBA
- H. ducreyi
- H. influenzae
- H. aegyptius
- H . parainfluenzae
48which Haemophilus spp. appears as a "school of fish" or fingerprint arrangements microscopically
- H. aphrophilius
- H. ducreyi
- H. aegyptius
- H. parainfluenzae
49which Haemophilus species is ALA positive and X negative
- H. parainfluenzae
- H. influenzae
- H. aegyptius
- H. ducreyi
50how can you differentiate between H. influenzae and H. aegyptius
- H. influenzae is beta hemolytic on HBA, H. aegyptius is gamma hemolytic
- H. influenzae is xylose positive, H. aegyptius is xylose negative
- H. influenzae is XV positive, H. aegyptius is XV negative
- H. influenzae is xylose negative, H. aegyptius is xylose positive
51which is a common trait in infections casued by Bartonella spp.
- infections typically manifest in the lungs
- they are transmitted between poeple via infected blood products
- they induce excessive apoptosis
- they infect RBCs/vascular endothelial cells
52a 23 year old female presents with a wound caused by a cat scratch. there is a papule at the wound site, she is experiencing fever, swollen lymph nodes, anorexia, malaise, and arthralgia. what is the likely cause
- Capnocytophaga cynodegmi
- Bartonella henselae
- Bartonella bacilliformis
- Pasteurella multocida
53a student returns from studying abroad in Peru and presents with acute fever and anemia. he is sent away with antibiotics but returns months later with red-purple hemangiomatous lesions along the extremities. what is likely the casuative agent
- Bartonella quintana
- Bartonella henselae
- Brucella suis
- Bartonella bacilliformis
54an unhoused man presents with myalgia, splenomegaly, shin pain, and a relapsing fever. he states that he recently dealt with a case of lice and shows bites on his arms and torso. what is the causative agent
- Bartonella bacilliformis
- Bartonella henselae
- Brucella suis
- Bartonella quintana
55aside from viridians streptococci and the HACEK group, which organisms may be responsible for endocarditis?
- all of the above
- Pasteurella multocida
- Bartonella spp.
- Capnocytophaga spp.
56which organism can induce bacillary peliosis?
- Bartonella henslae
- Brucella suis
- Pseudomonas stutzeri
- Bordetella pertussis
57what is the best way to distinguish between Bartonella spp?
- none of the above
- string test/O/129 sensitivity
- oxidase/catalase testing
- PCR or serological testing
58what about Brucella makes it difficult to grow?
- it has complex growth requirements
- all of the above
- it's an intracellular organism
- it is a slow growing organism
59T/F - DNA analysis has found that there is only one species of Brucella (B. melitensis) with several biovariants, but the biovariants are still referred to by their original species designation
60from which animals did B. melitensis originate?
- goat, sheep
- swine
- humans
- cattle
61from which animals did B. suis originate?
- goat, sheep
- swine
- cattle
- dogs
62from which animals did B. abortis originate?
- cattle
- goat, sheep
- swine
- dogs
63a lab tech presents with an undulant fever after handling patient specimens that came from a dairy farmer. upon culturing, the lab tech's result showed they were infected by a pathogen positive for all of the following: catalase, oxidase, h2s, urease, and nitrate. which is the most likely?
- B. abortus
- B. melitensis
- B suis
- B. canis
64which organism fits the following:
- "gull wing" Gram stain
- microaerophilic/capnophilic requirements
- grows at a wide variety of temperatures
- enterotoxins produced may yield diarrhea
- Campylobacter fetus
- Campylobacter jejuni
- Escerhichia coli
- Campylobacter coli
65describe the microscopic appearance of Helicobacter pylori
- gull wing/wavy shaped GNR
- pleomorphic with bipolar staining
- flowery looking arrangement, GNR
- small, curved U/C shaped GNR
66which organism can be identified through the urea breath test or rapid urease reaction using a gastic biopsy specimen as a result of the lesions it causes?
- B. suis
- C. jejuni
- L. pneumophila
- H. pylori
67which media is best for isolating Legionella pneumophila?
- cetrimide
- HBS (horse blood)
- none of the above
- BCYE (buffered charcoal-yeast extract agar)
68which organism fits the following:
- growth on BCYE, growth resembles a broken windshield/speckly glass
- causative agent of Pontiac fever
- pale yellowreen fluorescence under long wave fluorescent lights
- Legionella pneumophila
- Francisella tularensis
- Bordetella pertussis
- Brucella suis
69which organism fits the following:
- best grown on media with charcoal
- Bordet-Gengou with 20% blood yields "mercury drop" growth
- Regan-Lowe media used for transport yields white, pearly colonies
- generally IDed with PCR, direct fluorescent antibody, or slide agglutination
- Bartonella quintana
- Brucella suis
- Bordetella pertussis
- Bartonella henselae
70etiologic agent of whooping cough, a disease that primarily infects children under 5
- Bordetella pertussis
- Brucella melitensis
- Bartonella canis
- Bartonella quintana
71which organism fits the following:
- grows best on agar with cysteine (BCYE or others)
- highly infectious
- uses direct fluorescence and serum agglutination for ID
- Bordetella pertussis
- Streptobacillus monoliformis
- Bartonella canis
- Francisella tularensis
72glandular fever, tick fever, deer fly fever and rabbit fever are all the same disease, caused by ______________
- Brucella spp.
- F. tularensis
- B. quintana
- B. henselae
73what causes L forms?
- autophagy
- loss of cell wall
- phagocytosis by macrophages
- disintegration of nucleus
74which organism fits the following:
- microaerophilic
- GNR that forms chains resembling beads (or perhaps an earthworm)
- shows irregular, fried egg looking colonies with L forms on BHI
- yields "fluff balls" or "bread crumbs" in broth
- associated with rat bites in the USA
- Streptobacillus monoliformis
- Aggregatibacter aphrophilus
- Francisella tularensis
- Kingella kingae
75to which concentration(s) of O/129 is Aeromonas hydrophila resistant?
- 10 and 150 micrograms
- 10 micrograms
- 150 micrograms
- it is sensitive to all
76describe the appearance of Aeromonas hydrophila on CIN agar
- fried egg colonies
- wide and feathery colonies
- bullseye colonies
- flower shaped colonies